LOCUS       sequence-241140-        6375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 183_pAAV-ProD14-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        734..765
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      819..828
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             825..1751
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1755..1757,1758..1760,1761..2474)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1755..1757,1758..1760,1761..2471)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1800..1821)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2061..2080)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2502..3090
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2555..2575)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2973..2984)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3122..3598
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3321..3340)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3638..3778
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3638..3767
                     /label=AAV2 ITR
     rep_origin      3853..4308
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3940..3959)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4150..4171
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4325..4344)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4444..4466
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4504..4522)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4590..4694
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4695..4763,4764..5555)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4913..4932)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5726..6314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6215..6234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag
      181 aagaggaagt caaacaggac tagtcatcga taggctagcc agcttaatta atcagattct
      241 tgattgtcta ggctacgagg aggagggggc tagggatgaa gtcacttgag agatctgttt
      301 taaatccttg tcaaagtctg tttcgctgtg tttttatact ctaggccagg tgaaatttta
      361 ggaatgtcag gatatttgag caaacagtct agatctgggg caggctgacg aggaacatgt
      421 ggccaccacg gaacacgaaa cttgtttctg tgagttagtg cagaggctcc cagcaggttg
      481 ggcagtctgg ccctggcctc ctgccaatta gaattcgata ttcaagtgca aggggaaatg
      541 ctaggggtca aaccatgcga cagagatggg gttcagggag ataggggaga ggaagctaaa
      601 aaaaaattaa gtatacatgg gaatgcctta aagaatttca gagtgggcag caaagtaact
      661 gccgggtttc ctctgcttac agggcactag tattcaaaac caggggatcc tcacatggtc
      721 ctgctggagt tagtagaggg tatataatgg aagctcgact tccagctatc acatccactg
      781 tgttgttgtg aactggaatc cactataggc catgatccgc caccatggat tatggaggcg
      841 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa cccagtagtc gtcaatggct
      901 ctgtacttgt gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa
      961 acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
     1021 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg
     1081 tgtgcgctat cgagatggtc aaggtgattc tcgagttctt cttcgagttt aagaacccgt
     1141 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc gagtggcttc
     1201 tcacctgccc ggtcatttgc attcacctgt caaacctgac gggcttgtcc aacgactaca
     1261 gcaggcgcac catgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt
     1321 ccgccatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
     1381 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccacacc gtgccgaagg
     1441 gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
     1501 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg tacggctcca
     1561 ccgtcggcca caccatcatt gacctgatgt cgaagaactg ctggggtctg ctcggccact
     1621 acctgcgcgt gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca
     1681 aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
     1741 ctggcgcggt acccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1801 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1861 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1921 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1981 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2041 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2101 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2161 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     2221 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     2281 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2341 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     2401 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2461 agctgtacaa gtgataagaa ttcgatatca agcttatcga taatcaacct ctggattaca
     2521 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     2581 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     2641 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     2701 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     2761 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     2821 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     2881 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga
     2941 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3001 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3061 gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatcta
     3121 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc
     3181 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     3241 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     3301 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     3361 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     3421 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     3481 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     3541 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     3601 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     3661 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     3721 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     3781 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     3841 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3901 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3961 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4021 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     4081 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4141 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     4201 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4261 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     4321 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4381 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4441 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     4501 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4561 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4621 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4681 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4741 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4801 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4861 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4921 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4981 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5041 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5101 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5161 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5221 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5281 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5341 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5401 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5461 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5521 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5581 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5641 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6361 cttttgctca catgt
//
