LOCUS       sequence-24117-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme S44M WT*
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(712..734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    723..739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(747..753,754..769,770..775))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctangaaaaa gcccgctcat taggcgggct cagatctgct catgtttgac agcttatcat
       61 cgataagctt cggcaataaa ttcttgatat gttttcatat gtttccttta aatattttaa
      121 ttagtaattg tctattcaag taattgtgaa tatactatca caattccaag agaaagtaaa
      181 cagctttata gatttttata cgcgtcccaa gtgccagttc taaacgttgt aatgactcgt
      241 tttgcgcgat taggtgtttg attataccat ctacttttag ctaagttaac tgctgcttca
      301 tcccagcgtt tttgttgaag catacgtaaa gagttagtaa atcctgccac accggtttct
      361 cccatttgga aaaccatatt aatcaatgca gcgcgacgaa ccgcatcaag agaatcataa
      421 accggtttta atttagcatt tctcagaatt ccgcgaacag cagcatcaac atcctgatta
      481 aagagttttt cagcctcatc ttttgtaatt acaccattag tattacgccc aatagcttta
      541 tctaattcca ttttagcagc attaagtgat ggactttttg taagcaaatg accgatgcca
      601 atagtgtaat agccttctgt gtctttatag attttaagtc taagaccttc atctatacgt
      661 aacatttcaa atatattcat aatacctcct aagcatcgat ggatcctgtt tcctgtgtga
      721 aattgttatc cgctcacaat tccacacatt atacgagccg atgattaatt gtcaacaggg
      781 ggatggggag taagctcatc gatggatcga nccggnttnc cnggggggaa atggttancc
      841 gctcncaatt ccncacatta tacgagccgg aagcataaag tggnaaagcc cgggggtgcc
      901 taangagtgn agctanctta cattaattgc gtngcgctcn ctgcccgctt tccagtcggg
      961 aaacctgncg ggccaggann ccatcgaang gnggcaaanc ctttcgcggg nanggcatga
     1021 nancncccgg gaaaaaagnc canttcnggg ggggggnaag gngaaa
//
