LOCUS       sequence-241184-       11809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-001428gp160-754*
FEATURES             Location/Qualifiers
     source          1..11809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1342..3131
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3691..3713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3856..3990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3893..3912)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3947..3966
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6299..6323)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6320..6523)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6349..6369)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6524..6903
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7557..7605
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7630..7649
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7647..8684)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(8727..8872)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(9303..9891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9383..9402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10062..10853,10854..10922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10685..10704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10923..11027)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11095..11113
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaacaggagc aggagcagga gcgggagggg caggagcagg aggggcagga gcaggaggag
       61 gggcaggagc aggaggggca ggagcaggag gtggaggccg gggtcgagga ggcagtggag
      121 gccggggtcg aggaggtagt ggaggccggg gtcgaggagg tagtggaggc cgccggggta
      181 gaggacgtga aagagccagg gggggaagtc gtgaaagagc cagggggaga ggtcgtggac
      241 gtggagaaaa gaggcccagg agtcccagta gtcagtcatc atcatccggg tctccaccgc
      301 gcaggccccc tccaggtaga aggccatttt tccaccctgt aggggaagcc gattattttg
      361 aataccacca agaaggtggc ccagatggtg agcctgacgt gcccccggga gcgatagagc
      421 agggccccgc agatgaccca ggagaaggcc caagcactgg accccggggt cagggtgatg
      481 gaggcaggcg caaaaaagga gggtggtttg gaaagcatcg tggtcaagga ggttccaacc
      541 cgaaatttga gaacattgca gaaggtttaa gagctctcct ggctaggagt cacgtagaaa
      601 ggactaccga cgaaggaact tgggtcgccg gtgtgttcgt atatggaggt agtaagacct
      661 ccctttacaa cctaaggcga ggaactgccc ttgctattcc acaatgtcgt cttacaccat
      721 tgagtcgtct cccctttgga atggcccctg gacccggccc acaacctggc ccgctaaggg
      781 agtccattgt ctgttatttc atggtctttt tacaaactca tatatttgct gaggttttga
      841 aggatgcgat taaggacctt gttatgacaa agcccgctcc tacctgcaat atcagggtga
      901 ctgtgtgcag ctttgacgat ggagtagatt tgcctccctg gtttccacct atggtggaag
      961 gggctgccgc ggagggtgat gacggagatg acggagatga aggaggtgat ggagatgagg
     1021 gtgaggaagg gcaggagtga tgtaacttgt taggagacgc cctcaatcgt attaaaagcc
     1081 gtgtattccc ccgcactaaa gaataaatcc ccagtagaca tcatgcgtgc tgttggtgta
     1141 tttctggcca tctgtcttgt caccattttc gtcctcccaa catggggcaa ttgggcatac
     1201 ccatgttgtc acgtcactca gctccgcgct caacaccttc tcgcgttgga aaacattagc
     1261 gacatttacc tggtgagcaa tcagacatgc gacggcttta gcctggcctc cttaaattca
     1321 cctaagaatg ggagcaacca gcaggaaaag gacaagcagc gaaaattcac gcccccttgg
     1381 gaggtggcgg catatgcaaa ggatagcact cccactctac tactgggtat catatgctga
     1441 ctgtatatgc atgaggatag catatgctac ccggatacag attaggatag catatactac
     1501 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
     1561 ccagatataa attaggatag catatactac ccagatatag attaggatag catatgctac
     1621 ccagatatag attaggatag cctatgctac ccagatatag attaggatag catatgctac
     1681 ccagatatag attaggatag catatgctat ccagatattt gggtagtata tgctacccag
     1741 atataaatta ggatagcata tactacccta atctctatta ggatagcata tgctacccgg
     1801 atacagatta ggatagcata tactacccag atatagatta ggatagcata tgctacccag
     1861 atatagatta ggatagccta tgctacccag atataaatta ggatagcata tactacccag
     1921 atatagatta ggatagcata tgctacccag atatagatta ggatagccta tgctacccag
     1981 atatagatta ggatagcata tgctatccag atatttgggt agtatatgct acccatggca
     2041 acattagccc accgtgctct cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt
     2101 ggcgctcagg cgcaagtgtg tgtaatttgt cctccagatc gcagcaatcg cgcccctatc
     2161 ttggcccgcc cacctactta tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc
     2221 aagtggtttg accgcagtgg ttagcggggt tacaatcagc caagttatta cacccttatt
     2281 ttacagtcca aaaccgcagg gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt
     2341 tcaaaaaaaa gagtggccac ttgtctttgt ttatgggccc cattggcgtg gagccccgtt
     2401 taattttcgg gggtgttaga gacaaccagt ggagtccgct gctgtcggcg tccactctct
     2461 ttccccttgt tacaaataga gtgtaacaac atggttcacc tgtcttggtc cctgcctggg
     2521 acacatctta ataaccccag tatcatattg cactaggatt atgtgttgcc catagccata
     2581 aattcgtgtg agatggacat ccagtcttta cggcttgtcc ccaccccatg gatttctatt
     2641 gttaaagata ttcagaatgt ttcattccta cactagtatt tattgcccaa ggggtttgtg
     2701 agggttatat tggtgtcata gcacaatgcc accactgaac cccccgtcca aattttattc
     2761 tgggggcgtc acctgaaacc ttgttttcga gcacctcaca tacaccttac tgttcacaac
     2821 tcagcagtta ttctattagc taaacgaagg agaatgaaga agcaggcgaa gattcaggag
     2881 agttcactgc ccgctccttg atcttcagcc actgcccttg tgactaaaat ggttcactac
     2941 cctcgtggaa tcctgacccc atgtaaataa aaccgtgaca gctcatgggg tgggagatat
     3001 cgctgttcct taggaccctt ttactaaccc taattcgata gcatatgctt cccgttgggt
     3061 aacatatgct attgaattag ggttagtctg gatagtatat actactaccc gggaagcata
     3121 tgctacccgt ttagggttaa caagggggcc ttataaacac tattgctaat gccctcttga
     3181 gggtccgctt atcggtagct acacaggccc ctctgattga cgttggtgta gcctcccgta
     3241 gtcttcctgg gcccctggga ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa
     3301 gagttacaca taaaggcaat gttgtgttgc agtccacaga ctgcaaagtc tgctccagga
     3361 tgaaagccac tcagtgttgg caaatgtgca catccattta taaggatgtc aactacagtc
     3421 agagaacccc tttgtgtttg gtcccccccc gtgtcacatg tggaacaggg cccagttggc
     3481 aagttgtacc aaccaactga agggattaca tgcactgccc cgcgaagaag gggcagagat
     3541 gtcgtagtca ggtttagttc gtccggggcg gggatcgatc ctctagagtc gacctcatgg
     3601 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     3661 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3721 cgtcatcacc gaaacgcgcg aggcagccgg atcataatca gccataccac atttgtagag
     3781 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     3841 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3901 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3961 ctcatcaatg tatcttatca tgtctggatc cggccttgcc ggcctcgagt tatcacagga
     4021 aaccgttcac aagcctcacg ctcctatctc tatcctgctc acctccctct tcttcgattc
     4081 tcccgagtct gtcggggcca gtcgggtttg gggtcaatgt ttggaaagac aatggggaat
     4141 acccctgtct tactcggttg acgatagaca ataccgcaaa aatgatgcga agaccaatca
     4201 acccacctac tatcattatg aaaattttta tataccagag ccaatttgag atatcaaacc
     4261 atgaccaaag attcttccag gaatcgagcg cgaggaggtc ttgctcgttc ctctcttgtt
     4321 gattctgaga gtcctccaga aggcgataaa taattccggt atagttgctc acctctctat
     4381 cccactgcat ccatgtcata ttgtcccaga tgtccgtgag acttttgtta gaccaactag
     4441 aattccaagg cacggcagta gtacagatca attttccact gcacccccag ataccgagga
     4501 gttgttggtc cttcaaatat ctttcgatag caagtacccg ggtttgaagc tgtttgatcc
     4561 cccaaaccgt aagctgcaac aagtgttgtt gagcctcgat agcttgcaag agatttgatt
     4621 gctgctgaac gattcctgac agcaattgtc tggcctgcac tgtcaaagtg attgatgcgg
     4681 ctcccatagt agatccagct gcccccaaga aaccgaggaa aacggcgccg aggccgaccg
     4741 ctcgtttttc cctctcaaca acccgtcgct tcgccctcgt cggggcgacg cccaggggtt
     4801 taatctcaac gaccttgtac ttataaagtt cgcttctcca gttgtcccgc atatcccccc
     4861 cgccggggcg gaatatctca gtgttgttat tttttcctcc gtccctcacg agcaagagcc
     4921 cggttatatt agaattacaa gtaatgtttc cagctattgg tggagcatac atcgctctcc
     4981 ctacctcttg ccacatattt ataatttgct ttattctaca gggcagaatt atcgtggaat
     5041 tgctgttatt cgtgccattg ggcatatatg ttccgttcgg catatatgtg gagttgaaaa
     5101 gcccagacgt attgcagtaa aaaaattctc ctctgcagtt aaaactatga gtcgttatct
     5161 ccaagtcgcc accactgcta gaagtaaatt tgatggtctt gtttggaaag tgctccgcaa
     5221 gcttttcaga cactctcctc agcatttcat gccacttttt ttcggagatg ttgcagtggg
     5281 cctctcgaat gtttccgatt atgtctccag tagcatagaa tgtctggcct ggcccgattc
     5341 tgatactctt ccgggtgttg ttattcggtc tagtacacac gatctccacg ctctgatcca
     5401 ggtggacaat gatggtcttt acattatcgg tgaggttttc gcttctaatg ataatctcct
     5461 cttcggccaa tgaaccatta agcagcagtt gtgtgctaac aacgggctta atcccgtgag
     5521 tgcattgcac tgtactaacg ttgttgcaag aacctgtgcc gttaaatgtc ttattattgc
     5581 atttgagtat tgcatagcct gccggggtgc agtaatgtat tgggataggg tcgaaattta
     5641 cttttgggca cgcctgcgtg atggcagagg tgttacaatt gatgagaatg tacttagatg
     5701 cggaatttga atctcctctg ttctctctct ccaacggaac gaggtccagt cgatagaaca
     5761 gtgcatacgc tttttgtttc ttgtctctga tttcggtcgt tgtgttgaaa ctgcaattct
     5821 tcatttcatc gccgttgacc tgagtgacgt ttacctgagt tgtattcccc tgagtcgcgt
     5881 tgacctgtgt gcattccaag gttacgcaaa gtggcgtcag tttaacacaa ggcttgagac
     5941 tttgtgccca caaagaaatt acgtcctcgt gcatctggtc taccatatca ttcttccaca
     6001 tgttgaagtt ttccgtgacg tttcccaaca ccatctcttg ggggttaggg tccgtaggta
     6061 cgcaagcgtg ggttgcccac acgttatgga cttcggtctc gtaggccttc gcatcagacg
     6121 cgcaaaacaa agttgtgcgc gcctccttcc acacgggaac cccatagtaa acggttaccc
     6181 agagattctc gactgccagc acacttgcta cgagcatgcc caggaggtac aacgtggcca
     6241 acggttgcag gctccccata ggcatggtgg cgctagcagc tggtacccag cttctagaga
     6301 tctgacggtt cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
     6361 ccatttgcgt caacggggcg gggttattac gacattttgg aaagtcccgt tgattttggt
     6421 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     6481 accgctatcc acgcccattg gtgtactgcc aaaaccgcat caccatggta atagcgatga
     6541 ctaatacgta gatgtactgc caagtaggaa agtcccgtaa ggtcatgtac tgggcataat
     6601 gccaggcggg ccatttaccg tcattgacgt caataggggg cggacttggc atatgataca
     6661 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     6721 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     6781 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     6841 atgggctatg aactaatgac cccgtaattg attactatta ataactagtc aataatcaat
     6901 gtcaacatgg cggtcatatt ggacatgagc caatataaat gtacatatta tgatatagat
     6961 acaacgtatg caatggccaa tagccaatat tgatttatgc tatataacca atgactaata
     7021 tggctaattg ccaatattga ttcaatgtat agatcttcca tacctaccag ttctgcgcct
     7081 gcagcaatgg caacaacgtt gcccggatct gcgatgataa gctgtcaaac atgagaattg
     7141 gtcgactagc ttggcacgcc agaaatccgc gcggtggttt ttgggggtcg ggggtgtttg
     7201 gcagccacag acgcccggtg ttcgtgtcgc gccagtacat gcggtccatg cccaggccat
     7261 ccaaaaacca tgggtctgtc tgctcagtcc agtcgtggac ctgaccccac gcaacgccca
     7321 aaataataac ccccacgaac cataaaccat tccccatggg ggaccccgtc cctaacccac
     7381 ggggccagtg gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc
     7441 acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg
     7501 gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac
     7561 aaacgaccca acacccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc
     7621 cttccggtat tgtctccttc cgtgtttcag ttagcctccc ccatctcccg atccggacga
     7681 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
     7741 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
     7801 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
     7861 tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc
     7921 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
     7981 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
     8041 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
     8101 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
     8161 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
     8221 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
     8281 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg
     8341 accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
     8401 gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc
     8461 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
     8521 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
     8581 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
     8641 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctca ttgccccccg
     8701 ggatctgcgg cacgctgttg acgctgttaa gcgggtcgct gcagggtcgc tcggtgttcg
     8761 aggccacacg cgtcacctta atatgcgaag tggacctcgg accgcgccgc cccgactgca
     8821 tctgcgtgtt cgaattcgcc aatgacaaga cgctgggcgg ggtttgtgtc atcatagaac
     8881 taaagacatg caaatatatt tcttccgggg acaccgccag caaacgcgag caacgggcca
     8941 cggggatgaa gcagggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa
     9001 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
     9061 gtccgccatc tccagcagcc gcacgcggcg catctcgggg ccgacgcgct gggctacgtc
     9121 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     9181 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     9241 cagggacagc ttcaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9301 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9361 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9421 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9481 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9541 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9601 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9661 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9721 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9781 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9841 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9901 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9961 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10021 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10081 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10141 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10201 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10261 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10321 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
    10381 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10441 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10501 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10561 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10621 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
    10681 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10741 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10801 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10861 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10921 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10981 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11041 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    11101 cgtatcacga ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa
    11161 gctgatcctc acaggccgca cccagctttt cttccgttgc cccagtagca tctctgtctg
    11221 gtgaccttga agaggaagag gaggggtccc gagaatcccc atccctaccg tccagcaaaa
    11281 agggggacga ggaatttgag gcctggcttg aggctcagga cgcaaatctt gaggatgttc
    11341 agcgggagtt ttccgggctg cgagtaattg gtgatgagga cgaggatggt tcggaggatg
    11401 gggaattttc agacctggat ctgtctgaca gcgaccatga aggggatgag ggtggggggg
    11461 ctgttggagg gggcaggagt ctgcactccc tgtattcact gagcgtcgtc taataaagat
    11521 gtctattgat ctcttttagt gtgaatcatg tctgacgagg ggccaggtac aggacctgga
    11581 aatggcctag gagagaaggg agacacatct ggaccagaag gctccggcgg cagtggacct
    11641 caaagaagag ggggtgataa ccatggacga ggacggggaa gaggacgagg acgaggaggc
    11701 ggaagaccag gagccccggg cggctcagga tcagggccaa gacatagaga tggtgtccgg
    11761 agaccccaaa aacgtccaag ttgcattggc tgcaaaggga cccacggtg
//
