LOCUS       sequence-241191-        9230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-LpCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..9230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          666..1682
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1606..1628
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1690..1709
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1743..1761
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1773..1793
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5724..5744
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5751..5840
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5862..5915
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5916..5918,5919..5921,5922..6635)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5916..5918,5919..5921,5922..6632)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5961..5982)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6222..6241)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6569..6590
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6678..6799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6715..6734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6769..6788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6842..6858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6842..6858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6855..6877)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6866..6882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6890..6896,6897..6914,6915..6920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6935..6956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7073..7090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7244..7832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7324..7343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8003..8794,8795..8863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8626..8645
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8864..8968)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9047..9066)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
      421 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
      481 gcagcgatgg gggcgggggg ggggggggns gcgcgccrgg sggggsgggg sggggsgggg
      541 ggsggggsgg ggsgrggsgg rgrggkgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
      721 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
      781 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
      841 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
      901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
      961 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     1021 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     1081 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     1141 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     1201 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     1261 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     1321 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     1381 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     1441 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1501 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     1561 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     1621 gtgtgaccgg cggctctagc gcctctgcta accatgttca tgccttcttc tttttcctac
     1681 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gctagtgaat
     1741 tctaatacga ctcactatag gccgccacca tgcccaagaa gaagaggaag gttcccggga
     1801 tgatcatgaa caacgtgacc ggcgacttca gcgagttcgt ggccatcagc aaggtgcaga
     1861 agaccctgcg caacgagctg cgccccaccc ccctgaccat gaagcacatc aagcagaagg
     1921 gcatcatcac cgaggacgag tacaagaccc agcagagcct ggagctgaag cgcatcgccg
     1981 acggctacta ccgcgactac atcacccaca agctgaacga caccaacaac ctggacttcc
     2041 gcaacctgtt cgaggccatc gaggagaagt acaagaagaa cgacaaggac aaccgcgaca
     2101 agctggacct ggtggagaag agcaagcgcg gcgagatcgc caagctgctg agcgccgacg
     2161 acaacttcaa gagcatgttc gaggccaagc tgatcaccca gctgctgccc gtgtacgtgg
     2221 agcagaacta catcggcgag gacaaggaga aggccctgga gaccatcgcc ctgttcaagg
     2281 gcttcaccac ctacttcacc gactacttca acatccgcaa gaacatgttc aaggagaacg
     2341 gcggcgccag cagcatctgc taccgcatcg tgaacgtgaa cgccagcatc ttctacgaca
     2401 acctgaagac cttcatgtgc atcaaggaga aggccgagac cgagatcgcc ctgatcgagg
     2461 aggagctgac cgagctgctg gacagctggc gcctggagca catcttcagc gaggactact
     2521 acaacgagct gctggcccag aagggcatcg actactacaa ccagatctgc ggcgacgtga
     2581 acaagcacat gaacctgtac tgccagcaga acaagctgaa ggccaacgtg ttcaagatga
     2641 ccaagctgca gaagcagatc atgggcatca gcgagaaggc cttcgagatc ccccccatgt
     2701 accagaacga cgaggaggtg tacgccgcct tcaacggctt catcagccgc ctggaggagg
     2761 tgaagctgat cgaccgcctg ggcaacgtgc tgcagaacag caacatctac gacaccgcca
     2821 agatctacat caacgcccgc tgctacacca acgtgagcag ctacgtgtac ggcggctggg
     2881 gcgtgatcga gagcgccatc gagcgctact ggtacaacac catcgccggc aagggccaga
     2941 gcaaggccaa gaagatcgag aaggccaaga aggacaacaa gttcatgagc gtgaaggagc
     3001 tggacagcat cgtgagcgac tacgagcccg actacttcaa cgccagcaac atggacgacg
     3061 acaacagcgg ccgcgccttc agcggccacg gcgtgctggg ctacttcaac aagatgagca
     3121 agctgctggc caacatgagc ctgcacacca tcacctacga cagcggcgac agcctgatcg
     3181 agaacaagga gaccgccctg aacatcaaga aggacctgga cgacatcatg agcatctacc
     3241 actggctgca gaccttcatc atcgacgagg tggtggagaa ggacaacgcc ttctacgccg
     3301 agctggagga catctactac gagctggaga acgtggtgac cctgtacgac cgcatccgca
     3361 actacgtgac ccgcaagccc tacagcaccc agaagttcaa gcttaacttc gccagcccca
     3421 ccctggccag cggctggagc cgcagcaagg agttcgacaa caacgccatc atcctgctgc
     3481 gcaacaacaa gtactacatc gccatcttca acgtgaacaa caagcccgac aagcagatca
     3541 tcaagggcag cgaggagcag cgcctgagca ccgactacaa gaagatggtg tacaacctgc
     3601 tgcccggccc caacaagatg ctgccctggg tgttcatcaa gagcaacacc ggcaagcgcg
     3661 actacaaccc cagcagctac atcctggagg gctacgagaa gaaccgccac atcaagagca
     3721 gcggcaactt cgacatcaac tactgccacg acctgatcga ctactacaag gcctgcatca
     3781 acaagcaccc cgagtggaag aactacggct tcaagttcaa ggagaccacc cagtacaacg
     3841 acatcggcca gttctacaag gacgtggaga agcagggcta cagcatcagc tgggcctaca
     3901 tcagcgaggc cgacatcaac cgcctggacg aggagggcaa gatctacctg ttcgagatct
     3961 acaacaagga cctgagcagc cacagcaccg gcaaggacaa cctgcacacc atgtacctga
     4021 agaacatctt cagcgaggac aacctgaaga acatctgcat cgagctgaac ggcaacgccg
     4081 agctgttcta ccgcaagagc agcatgaagc gcaacatcac ccacaagaag gacaccgtgc
     4141 tggtgaacaa gacctacatc aacgaggccg gcgtgcgcgt gagcctgacc gacgaggact
     4201 acatcaaggt gtacaactac tacaacaacg actacgtgat cgacgtggag aaggacaaga
     4261 agctggtgga gatcctggag cgcatcggcc accgcaagaa ccccatcgac atcatcaagg
     4321 acaagcgcta caccgaggac aagtacttcc tgcacttccc catcaccatc aactacggcg
     4381 tggacgacga gaacatcaac gccaagatga tcgagtacat cgccaagcac aacaacatga
     4441 acgtgatcgg catcgaccgc ggcgagcgca acctgatcta catcagcgtg atcaacaaca
     4501 agggcaacat catcgagcag aagagcttca acctggtgaa caactacgac tacaagaaca
     4561 agctgaagaa catggagaag acccgcgaca acgcccgcaa gaactggcag gagatcggca
     4621 agatcaagga cgtgaagaac ggctacctga gcggcgtgat cagcaagatc gcccgcatgg
     4681 tggtggacta caacgccatc atcgtgatgg aggacctgaa ccgcggcttc aagcgcggcc
     4741 gcttcaaggt ggagcgccag gtgtaccaga agttcgagaa catgctgatc agcaagctga
     4801 actacctggt gttcaaggag aagaaggccg acgagaacgg cggcatcctg aagggctacc
     4861 agctgaccta cctgcccaag agcgccctgc agatcggcaa gcagtgcggc tgcatcttct
     4921 acgtgcccgc cgcctacacc agcaagatcg accccgccac cggcttcatc aacatcttcg
     4981 acttcaagaa gtacagcggc agcgccatca acgccaaggt gaaggacaag aaggagttcc
     5041 tgatgagcat gaacagcatc cgctacgtga acgagggcag cgccgagtac gagaagatcg
     5101 gccaccgcca gctgttcgcc ttcagcttcg actacaacaa cttcaagacc tacaacgtga
     5161 gcatccccgt gaacgagtgg accacctaca cctacggcga gcgcatcaag aagctgtaca
     5221 aggacggccg ctggagcggc agcgaggtgc tgaacctgac cgaggacctg atcgagctga
     5281 tggagcagta cggcatcgag tacaaggacg gccacgacat ccgcgaggac atcagccaca
     5341 tggacgagat gcgcaacgcc gacttcatct gcaacctgtt cgagaagttc aagtacaccg
     5401 tgcagctgcg caacagcaag agcgaggccg agggcgacga ctacgaccgc ctggtgagcc
     5461 ccgtgctgaa cagccacaac ggcttcttcg acagcagcga ctacaaggag aacgagaaga
     5521 gcgacgacat catcgacgac aagcagatca tgcccaagga cgccgacgcc aacggcgcct
     5581 actgcatcgc cctgaagggc ctgtacgaga tcaacaagat caaggagaac tggagcgacg
     5641 acaagaagct gaaggagagc gagctgtaca tcggcgtgac cgagtggctg gactacatcc
     5701 agaaccgccg cttcgaggct agcccaaaga agaagaggaa agtctctaga tacccttatg
     5761 atgttccaga ttatgccgga tacccatacg atgtccctga ctatgcaggc tcctaccctt
     5821 atgacgtccc agactacgcc ggatccaggt ccggcggcgg agagggcaga ggaagtcttc
     5881 taacatgcgg tgacgtggag gagaatcccg gcccaatggt gagcaagggc gaggagctgt
     5941 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     6001 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     6061 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     6121 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     6181 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     6241 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     6301 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     6361 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     6421 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     6481 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     6541 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     6601 ggatcactct cggcatggac gagctgtaca agtaactgca gcgcggggat ctcatgctgg
     6661 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6721 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6781 aactcatcaa tgtatcttat taattaacgt cgacctctag ctagagcttg gcgtaatcat
     6841 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6901 ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt gagctaactc acattaattg
     6961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8881 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8941 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9001 acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca atctgctctg
     9061 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
//
