LOCUS       sequence-241196-        7388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) mEOS2 hYAP 1-2gamma
FEATURES             Location/Qualifiers
     source          1..7388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..694
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(2227..2247)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2232..2561
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2412..2547
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2635..3660
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2635..3660
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4177..4196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4458..4927
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(5042..5833,5834..5902))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5665..5684
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5903..6007)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6038..6507
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6572..6771
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             6972..7388
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7318..7340
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7356..7372
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ggccggatcc gccaccatga gtgcgattaa gccagacatg aagatcaaac tccgtatgga
       61 aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca agccttttga
      121 gggaaaacag agtatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga
      181 tatcctgacc actgcattcc attacggcaa cagggtattc gccaaatatc cagacaacat
      241 acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt
      301 cgaagacggg ggcatttgca ttgccagaaa cgacataaca atggaagggg acactttcta
      361 taataaagtt cgattttatg gtaccaactt tcccgccaat ggtccagtta tgcagaagaa
      421 gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg
      481 tgatattcat atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac
      541 tacttacaaa gctaaggaga agggtgtcaa gttaccaggc taccactttg tggaccactg
      601 cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt
      661 tgctcattct ggattgcctg acaatgccag acgagaattc atggatcccg ggcagcagcc
      721 gccgcctcaa ccggcccccc agggccaagg gcagccgcct tcgcagcccc cgcaggggca
      781 gggcccgccg tccggacccg ggcaaccggc acccgcggcg acccaggcgg cgccgcaggc
      841 accccccgcc gggcatcaga tcgtgcacgt ccgcggggac tcggagaccg acctggaggc
      901 gctcttcaac gccgtcatga accccaagac ggccaacgtg ccccagaccg tgcccatgag
      961 gctccggaag ctgcccgact ccttcttcaa gccgccggag cccaaatccc actcccgaca
     1021 ggccagtact gatgcaggca ctgcaggagc cctgactcca cagcatgttc gagctcattc
     1081 ctctccagct tctctgcagt tgggagctgt ttctcctggg acactgaccc ccactggagt
     1141 agtctctggc ccagcagcta cacccacagc tcagcatctt cgacagtctt cttttgagat
     1201 acctgatgat gtacctctgc cagcaggttg ggagatggca aagacatctt ctggtcagag
     1261 atacttctta aatcacatcg atcagacaac aacatggcag gaccccagga aggccatgct
     1321 gtcccagatg aacgtcacag cccccaccag tccaccagtg cagcagaata tgatgaactc
     1381 ggcttcaggt cctcttcctg atggatggga acaagccatg actcaggatg gagaaattta
     1441 ctatataaac cataagaaca agaccacctc ttggctagac ccaaggcttg accctcgttt
     1501 tgccatgaac cagagaatca gtcagagtgc tccagtgaaa cagccaccac ccctggctcc
     1561 ccagagccca cagggaggcg tcatgggtgg cagcaactcc aaccagcagc aacagatgcg
     1621 actgcagcaa ctgcagatgg agaaggagag gctgcggctg aaacagcaag aactgcttcg
     1681 gcaggcaatg cggaatatca atcccagcac agcaaattct ccaaaatgtc aggagttagc
     1741 cctgcgtagc cagttaccaa cactggagca ggatggtggg actcaaaatc cagtgtcttc
     1801 tcccgggatg tctcaggaat tgagaacaat gacgaccaat agctcagatc ctttccttaa
     1861 cagtggcacc tatcactctc gagatgagag tacagacagt ggactaagca tgagcagcta
     1921 cagtgtccct cgaaccccag atgacttcct gaacagtgtg gatgagatgg atacaggtga
     1981 tactatcaac caaagcaccc tgccctcaca gcagaaccgt ttcccagact accttgaagc
     2041 cattcctggg acaaatgtgg accttggaac actggaagga gatggaatga acatagaagg
     2101 agaggagctg atgccaagtc tgcaggaagc tttgagttct gacatcctta atgacatgga
     2161 gtctgttttg gctgccacca agctagataa agaaagcttt cttacatggt tataggtcga
     2221 ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2281 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2341 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     2401 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2461 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     2521 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgcc agctggggcg
     2581 ccctctggta aggttgggaa gccctgcaga tctgatcccg gggggcaatg agatatgaaa
     2641 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     2701 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     2761 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     2821 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     2881 tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     2941 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     3001 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     3061 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     3121 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     3181 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     3241 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     3301 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     3361 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     3421 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     3481 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     3541 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     3601 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     3661 agtagatgcc gaccgggatc tatcgatgcg gcgggtgtgg tggttacgcg taatctgctg
     3721 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3781 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3841 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3901 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3961 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4021 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4081 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4141 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4201 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4261 gcggagccta tggaaaaacg ccagcaacgc atcgataaaa taaaagattt tatttagtct
     4321 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     4381 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
     4441 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     4501 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     4561 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
     4621 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     4681 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     4741 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
     4801 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     4861 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     4921 tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta
     4981 aatggccata gtgcggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5041 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5101 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5161 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5221 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5281 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5341 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5401 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5461 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5521 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5581 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5641 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5701 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5761 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5821 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5881 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5941 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6001 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
     6061 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     6121 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     6181 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     6241 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     6301 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     6361 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     6421 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     6481 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
     6541 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
     6601 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
     6661 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
     6721 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
     6781 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
     6841 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
     6901 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     6961 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     7021 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     7081 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     7141 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     7201 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     7261 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     7321 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     7381 taggcgcc
//
