LOCUS       sequence-241198-        7352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression for funtional studies of cell growth promotion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Gaffney CJ, Oka T, Mazack V, Hilman D, Gat U, Muramatsu T, Inazawa 
            J, Golden A, Carey DJ, Farooq A, Tromp G, Sudol M
  TITLE     Identification, basic characterization and evolutionary analysis of 
            differentially spliced mRNA isoforms of human YAP1 gene.
  JOURNAL   Gene. 2012 Nov 10;509(2):215-22. doi: 10.1016/j.gene.2012.08.025. 
            Epub 2012 Aug 24.
  PUBMED    22939869
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2012.08.025"; journalName: "Gene"; date: 
            "2012-11-10- 10"; volume: "509"; issue: "2"; pages: "215-22"
COMMENT     Sequence Label: pBABE (hygro) mEOS2 hYAP 1-2beta
FEATURES             Location/Qualifiers
     source          1..7352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    409..608
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             809..1225
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1155..1177
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1193..1209
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1242..1919
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     primer_bind     complement(3416..3436)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3421..3750
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3601..3736
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3824..4849
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3824..4849
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     5366..5385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             5647..6116
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(6231..7022,7023..7091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6854..6873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7092..7196)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(7227..7352,1..344)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
       61 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
      121 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
      181 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
      241 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
      301 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
      361 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggcaa gctggccagc
      421 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg
      481 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      541 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      601 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      661 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      721 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      781 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
      841 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
      901 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
      961 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1021 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1081 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1141 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1201 agccctcact ccttctctag gcgccggccg gatccgccac catgagtgcg attaagccag
     1261 acatgaagat caaactccgt atggaaggca acgtaaacgg gcaccacttt gtgatcgacg
     1321 gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa gtcaaagagg
     1381 gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac ggcaacaggg
     1441 tattcgccaa atatccagac aacatacaag actattttaa gcagtcgttt cctaaggggt
     1501 attcgtggga acgaagcttg actttcgaag acgggggcat ttgcattgcc agaaacgaca
     1561 taacaatgga aggggacact ttctataata aagttcgatt ttatggtacc aactttcccg
     1621 ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact gagaaaatgt
     1681 atgtgcgtga tggagtgctg acgggtgata ttcatatggc tttgttgctt gaaggaaatg
     1741 cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt gtcaagttac
     1801 caggctacca ctttgtggac cactgcattg agattttaag ccatgacaaa gattacaaca
     1861 aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat gccagacgag
     1921 aattcatgga tcccgggcag cagccgccgc ctcaaccggc cccccagggc caagggcagc
     1981 cgccttcgca gcccccgcag gggcagggcc cgccgtccgg acccgggcaa ccggcacccg
     2041 cggcgaccca ggcggcgccg caggcacccc ccgccgggca tcagatcgtg cacgtccgcg
     2101 gggactcgga gaccgacctg gaggcgctct tcaacgccgt catgaacccc aagacggcca
     2161 acgtgcccca gaccgtgccc atgaggctcc ggaagctgcc cgactccttc ttcaagccgc
     2221 cggagcccaa atcccactcc cgacaggcca gtactgatgc aggcactgca ggagccctga
     2281 ctccacagca tgttcgagct cattcctctc cagcttctct gcagttggga gctgtttctc
     2341 ctgggacact gacccccact ggagtagtct ctggcccagc agctacaccc acagctcagc
     2401 atcttcgaca gtcttctttt gagatacctg atgatgtacc tctgccagca ggttgggaga
     2461 tggcaaagac atcttctggt cagagatact tcttaaatca catcgatcag acaacaacat
     2521 ggcaggaccc caggaaggcc atgctgtccc agatgaacgt cacagccccc accagtccac
     2581 cagtgcagca gaatatgatg aactcggctt caggtcctct tcctgatgga tgggaacaag
     2641 ccatgactca ggatggagaa atttactata taaaccataa gaacaagacc acctcttggc
     2701 tagacccaag gcttgaccct cgttttgcca tgaaccagag aatcagtcag agtgctccag
     2761 tgaaacagcc accacccctg gctccccaga gcccacaggg aggcgtcatg ggtggcagca
     2821 actccaacca gcagcaacag atgcgactgc agcaactgca gatggagaag gagaggctgc
     2881 ggctgaaaca gcaagaactg cttcggcagg tgaggccaca ggagttagcc ctgcgtagcc
     2941 agttaccaac actggagcag gatggtggga ctcaaaatcc agtgtcttct cccgggatgt
     3001 ctcaggaatt gagaacaatg acgaccaata gctcagatcc tttccttaac agtggcacct
     3061 atcactctcg agatgagagt acagacagtg gactaagcat gagcagctac agtgtccctc
     3121 gaaccccaga tgacttcctg aacagtgtgg atgagatgga tacaggtgat actatcaacc
     3181 aaagcaccct gccctcacag cagaaccgtt tcccagacta ccttgaagcc attcctggga
     3241 caaatgtgga ccttggaaca ctggaaggag atggaatgaa catagaagga gaggagctga
     3301 tgccaagtct gcaggaagct ttgagttctg acatccttaa tgacatggag tctgttttgg
     3361 ctgccaccaa gctagataaa gaaagctttc ttacatggtt ataggtcgac cctgtggaat
     3421 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3481 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3541 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3601 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3661 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3721 ggcttttttg gaggcctagg cttttgcaaa aagcttgcca gctggggcgc cctctggtaa
     3781 ggttgggaag ccctgcagat ctgatcccgg ggggcaatga gatatgaaaa agcctgaact
     3841 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     3901 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     3961 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     4021 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat ttagcgagag
     4081 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     4141 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     4201 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     4261 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     4321 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     4381 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     4441 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     4501 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     4561 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     4621 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     4681 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     4741 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     4801 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg
     4861 accgggatct atcgatgcgg cgggtgtggt ggttacgcgt aatctgctgc ttgcaaacaa
     4921 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4981 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5041 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5101 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5161 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5221 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5281 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5341 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5401 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5461 ggaaaaacgc cagcaacgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     5521 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     5581 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     5641 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     5701 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     5761 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     5821 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     5881 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     5941 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     6001 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     6061 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg
     6121 cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag
     6181 tgcggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6241 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6301 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6361 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6421 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6481 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6541 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6601 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6661 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6721 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6781 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6841 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6901 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6961 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7021 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7081 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7141 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7201 tttccccgaa aagtgccacc tgcagcctga atatgggcca aacaggatat ctgtggtaag
     7261 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     7321 gatatctgtg gtaagcagtt cctgccccgg ct
//
