LOCUS       sequence-241200-        8224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal fusions of C. albicans optimized mScarlet fluorescent 
            protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Frazer C, Hernday AD, Bennett RJ
  TITLE     Monitoring Phenotypic Switching in Candida albicans and the Use of 
            Next-Gen Fluorescence Reporters.
  JOURNAL   Curr Protoc Microbiol. 2019 Feb 12:e76. doi: 10.1002/cpmc.76.
  PUBMED    30747494
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Microbiol. 2019 Feb 12:e76. doi: 10.1002/cpmc.76."
COMMENT     Sequence Label: pRB897
FEATURES             Location/Qualifiers
     source          1..8224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             372..1643
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     protein_bind    complement(3900..3933)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(3935..3951)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3935..3951)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3988..4008)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3988..4006)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4027..4043)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4027..4043)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4040..4062)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4051..4067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4075..4081,4082..4099,4100..4105))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4120..4141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4258..4275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4429..5017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4509..4528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5237..5339
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5340..5999
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5406..5425)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6488..6592)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6619..7075
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6706..6725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6916..6937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7201..7223
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7215..7232
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7216..7232
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7242..7261
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7242..7260
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(8004..8037)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tgtctagcgt gctctgagtt gtagccactg aaaccacggt taataacatc caattttcgg
       61 atatacacat tctgtaatgc tggatgaaag ccatactggg tacaactaaa ctgggtgatg
      121 gagtcaccga acaacacaaa tttaccgtat tccatgattg ctatggttga gaattttttt
      181 tttttcttgt cccacgccat ttttcaaatt atgcagttga gaatgttagt ttttgtgtac
      241 accccgttcg ctgaatattt cggaataatt caaagattgg ggagtggggg aggcgataga
      301 cgaagacacg gtataaaaat gggcaaaatt ttccccaact ttttgcagtg gtttaactaa
      361 taatcgtcga catgccacaa tttgatatat tatgtaaaac accacctaag gtgcttgttc
      421 gtcagtttgt ggaaaggttt gaaagacctt caggtgagaa aatagcatta tgtgctgctg
      481 aactaaccta tttatgttgg atgattacac ataacggaac agcaatcaag agagccacat
      541 tcatgagcta taatactatc ataagcaatt cgttgagttt cgatattgtc aataaatcac
      601 tccagtttaa atacaagacg caaaaagcaa caattttgga agcctcatta aagaaattga
      661 ttcctgcttg ggaatttaca attattcctt actatggaca aaaacatcaa tctgatatca
      721 ctgatattgt aagtagtttg caattacagt tcgaatcatc ggaagaagca gataagggaa
      781 atagccacag taaaaaaatg cttaaagcac ttctaagtga gggtgaaagc atctgggaga
      841 tcactgagaa aatactaaat tcgtttgagt atacttcgag atttacaaaa acaaaaactt
      901 tataccaatt cctcttccta gctactttca tcaattgtgg aagattcagc gatattaaga
      961 acgttgatcc gaaatcattt aaattagtcc aaaataagta tttgggagta ataatccagt
     1021 gtttagtgac agagacaaag acaagcgtta gtaggcacat atacttcttt agcgcaaggg
     1081 gtaggatcga tccacttgta tatttggatg aatttttgag gaattctgaa ccagtcctaa
     1141 aacgagtaaa taggaccggc aattcttcaa gcaataaaca ggaataccaa ttattaaaag
     1201 ataacttagt cagatcgtac aataaagctt tgaagaaaaa tgcgccttat tcaatctttg
     1261 ctataaaaaa tggcccaaaa tctcacattg gaagacattt gatgacctca tttctttcaa
     1321 tgaagggcct aacggagttg actaatgttg tgggaaattg gagcgataag cgtgcttctg
     1381 ccgtggccag gacaacgtat actcatcaga taacagcaat acctgatcac tacttcgcac
     1441 tagtttctcg gtactatgca tatgatccaa tatcaaagga aatgatagca ttgaaggatg
     1501 agactaatcc aattgaggag tggcagcata tagaacagct aaagggtagt gctgaaggaa
     1561 gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac tacctttcat
     1621 cctacataaa tagacgcata taagagtgaa attctggaaa tctggaaatc tggttttgta
     1681 ttcttgttat tcttcttttt gttattacat atataacttg ttactttttt aaaaaaatct
     1741 ttgtatattt tataaatata taaaactaaa tttaagaaaa agagaaaaat gttttatttg
     1801 agagattgaa attttacttg aatttagctt agcttttata aagtattatt atgtaaaaaa
     1861 acaaaacaaa tatacattaa aaagttaaga ctataaaata gccacccaag gcatttctat
     1921 atcttgttgt tgttgttttc atcttctgta tcagaggaac ttattttatt attttcgtca
     1981 cgggtatttt ctcttgtttg atgattcatc ccattcattc catcataaaa tgtcgagcgt
     2041 caaaactaga gaataataaa gaaaacgatc ttttcaaaaa gaaaaaacct tttagttttc
     2101 ctttgttgtt gttgtgggtg tgtgctattt atattatata gtttactcat aataccataa
     2161 aatattcggt ttgattaggt tattttaata agctaatttg tttctaatcg tgtaatttat
     2221 gctgtgtata ttaagtagtg tgtgcactgc ccaaaaatgt ttgttgttta tagtcggtta
     2281 aagagaaaaa agaaaaaaag atccatacac acacgttaat tagttgttca acgtaataca
     2341 ctcatatttt gttcttattt gctttcggtc gctgttctca ccaagattta ttgccaacga
     2401 aacaattttt ttttatatat tttcagattt ttcttttttt cctttccttt ccttttctaa
     2461 ttttcactcc tggttttctt tctttcttag aaacattatc tcgatattaa tattaaaaaa
     2521 atataatcat tcaaaatgga cggtggtatg ttttagttta gcttcaattc taattgattg
     2581 attaatcagt tgattggttt caatatgaca aatgggtagg gtgggaaaac ttcattttca
     2641 attcagatca aacttttttg ttgtcgacat aatatttctc gtttgggatg ttactgtcac
     2701 attaataata cacacacatc agcttataat tttgaaagta atttatcaga tatgttgtga
     2761 cgatcagtgg aaatggctaa cttcaatgta tctgttcttc ccctttttca aagttcacgt
     2821 tttttgattg attgattgat ctgtcggcag tggtttcaaa accattcggt gagtaatcct
     2881 atcaatcaat gttacgacaa aaggctcaat attcaaaatt gcaatgtttt atgttttcct
     2941 acgtgtactt gtgcaaggca attgattcaa cattgctttt ggtgtttgac gagtttctag
     3001 tttggacttg tgttgttatc tgggctatac agatttcccg gctcactatg aatttttttt
     3061 ttcgacgctc agtgcacaca actataaaca acacaaacac aaacacagca agaaaaaaaa
     3121 aaaacgaaca ttgaattgaa accaagccaa ctgaaaaatt ccttatttaa atgactgtca
     3181 tactaaccca tttttataga agaagttgct gctttagtta tcgataacgg ttctcatatg
     3241 aaaatttcgg tgatccctga gcaggtggcg gaaacattgg atgctgagaa ccatttcatt
     3301 gttcgtgaag tgttcgatgt gcacctatcc gaccaaggct ttgaactatc taccagaagt
     3361 gtgagcccct accggaagga ttacatctcg gatgatgact ctgatgaaga ctctgcttgc
     3421 tatggcgcat tcatcgacca agagcttgtc gggaagattg aactcaactc aacatggaac
     3481 gatctagcct ctatcgaaca cattgttgtg tcgcacacgc accgaggcaa aggagtcgcg
     3541 cacagtctca tcgaatttgc gaaaaagtgg gcactaagca gacagctcct tggcatacga
     3601 ttagagacac aaacgaacaa tgtacctgcc tgcaatttgt acgcaaaatg tggctttact
     3661 ctcggcggca ttgacctctt cacgtataaa actagacctc aagtctcgaa cgaaacagcg
     3721 atgtactggt actggttctc gggagcacag gatgacgcct aacatatgtg aagtgtgaag
     3781 ggggagattt tcactttatt agatttgtat atatgtataa taaataaata aataagttaa
     3841 ataaataatt agataagggt ggtaattatt actatttaca atcaaaggtg gtcctgcagg
     3901 aagttcctat actttctaga gaataggaac ttcagatcca ctagttctag agcggccgcc
     3961 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     4021 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4081 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4141 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4201 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5101 aaaaaggatc ttcacctaga tccttttcga ccgaataaat acctgtgacg gaagatcact
     5161 tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt
     5221 ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     5281 gatcactacc gggcgtattt tttgagttgt cgagattttc aggagctaag gaagctaaaa
     5341 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     5401 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     5461 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     5521 acattcttgc ccgcctgatg aatgctcatc cggaattacg tatggcaatg aaagacggtg
     5581 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     5641 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     5701 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     5761 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     5821 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     5881 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     5941 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     6001 ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata
     6061 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
     6121 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
     6181 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
     6241 ggtaataatt gacgatatga tccttttttt ctgatcaaaa agtgctcatc attggaaaac
     6301 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6361 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6421 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6481 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6541 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6601 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6661 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6721 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6781 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6841 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6901 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6961 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaaatgag
     7021 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt
     7081 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     7141 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     7201 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     7261 gcgaattggg taccgggccc cccctcgagg gtggtagtgg tatggtttct aaaggtgaag
     7321 cagttattaa agaattcatg agattcaaag ttcatatgga aggtagtatg aatggtcatg
     7381 aatttgaaat tgaaggtgaa ggtgaaggta gaccatatga aggtactcaa actgctaaat
     7441 tgaaagttac taaaggtggt ccattaccat tttcatggga tattttgtca ccacaattca
     7501 tgtatggttc aagagctttt attaaacatc cagctgatat tccagattat tataaacaat
     7561 cttttccaga aggttttaaa tgggaaagag ttatgaattt cgaagatggt ggtgctgtta
     7621 ctgttactca agatacttca ttagaagatg gtactttgat ctataaagtt aaattgagag
     7681 gtactaattt tccaccagat ggtccagtta tgcaaaagaa aactatgggt tgggaagctt
     7741 ctactgaaag attatatcca gaagatggtg ttttgaaagg agatattaaa atggctttga
     7801 gattgaaaga tggtggtaga tatttggctg atttcaaaac tacttataaa gctaagaaac
     7861 cagttcaaat gccaggtgct tataatgttg atagaaaatt ggatattact tcacataatg
     7921 aagattatac tgttgttgaa caatatgaaa gaagtgaagg tagacatagt acaggtggta
     7981 tggatgaatt atattagctc gaggaagttc ctatactttc tagagaatag gaacttcgga
     8041 tccaataatg attggtttga tatttttgtc tagtaccatc tgtaccatta cacttaaatt
     8101 atctttatat ctgtctaact cgactgtctg gatttcattg atgtagtcgt atgcatcgtt
     8161 agttccaaaa aatattgtca tcaatttgat attggtttcc gactctaaaa tttttggaag
     8221 aatt
//
