LOCUS       sequence-241204-        7384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: mEos2-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..7384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3386..4066
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    4188..4309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4225..4244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4279..4298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4316..4771)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4453..4474)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4665..4684
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4798..4902
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4904..5261
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4904..5095
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4927..4947)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5112..5247
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5296..6090
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5296..6090
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5350..5369)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5960..5979
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6278..6297)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6322..6369
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6698..7286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7187..7206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgcacca tcatggacca
      661 ttatgattct cagcaaacca acgattacat gcagccagaa gaggactggg accgggacct
      721 gctcctggac ccggcctggg agaagcagca gagaaagaca ttcacggcat ggtgtaactc
      781 ccacctccgg aaggcgggga cacagatcga gaacatcgaa gaggacttcc gggatggcct
      841 gaagctcatg ctgctgctgg aggtcatctc aggtgaacgc ttggccaagc cagagcgagg
      901 caagatgaga gtgcacaaga tctccaacgt caacaaggcc ctggatttca tagccagcaa
      961 aggcgtcaaa ctggtgtcca tcggagccga agaaatcgtg gatgggaatg tgaagatgac
     1021 cctgggcatg atctggacca tcatcctgcg ctttgccatc caggacatct ccgtggaaga
     1081 gacttcagcc aaggaagggc tgctcctgtg gtgtcagaga aagacagccc cttacaaaaa
     1141 tgtcaacatc cagaacttcc acataagctg gaaggatggc ctcggcttct gtgctttgat
     1201 ccaccgacac cggcccgagc tgattgacta cgggaagctg cggaaggatg atccactcac
     1261 aaatctgaat acggcttttg acgtggcaga gaagtacctg gacatcccca agatgctgga
     1321 tgccgaagac atcgttggaa ctgcccgacc ggatgagaaa gccatcatga cttacgtgtc
     1381 tagcttctac cacgccttct ctggagccca gaaggcggag acagcagcca atcgcatctg
     1441 caaggtgttg gccgtcaacc aggagaacga gcagcttatg gaagactacg agaagctggc
     1501 cagtgatctg ttggagtgga tccgccgcac aatcccgtgg ctggagaacc gggtgcccga
     1561 gaacaccatg catgccatgc aacagaagct ggaggacttc cgggactacc ggcgcctgca
     1621 caagccgccc aaggtgcagg agaagtgcca gctggagatc aacttcaaca cgctgcagac
     1681 caagctgcgg ctcagcaacc ggcctgcctt catgccctct gagggcagga tggtctcgga
     1741 catcaacaat gcctggggct gcctggagca ggtggagaag ggctatgagg agtggttgct
     1801 gaatgagatc cggaggctgg agcgactgga ccacctggca gagaagttcc ggcagaaggc
     1861 ctccatccac gaggcctgga ctgacggcaa agaggccatg ctgcgacaga aggactatga
     1921 gaccgccacc ctctcggaga tcaaggcctt gctcaagaag catgaggcct tcgagagtga
     1981 cctggctgcc caccaggacc gtgtggagca gattgccgcc atcgcacagg agctcaatga
     2041 gctggactat tatgactcac ccagtgtcaa cgcccgttgc caaaagatct gtgaccagtg
     2101 ggacaatctg ggggccctaa ctcagaagcg aagggaagct ctggagcgga ccgagaaact
     2161 gctggagacc attgaccagc tgtacttgga gtatgccaag cgggctgcac ccttcaacaa
     2221 ctggatggag ggggccatgg aggacctgca ggacaccttc attgtgcaca ccattgagga
     2281 gatccaggga ctgaccacag cccatgagca gttcaaggcc accctccctg atgccgacaa
     2341 ggagcgcctg gccatcctgg gcatccacaa tgaggtgtcc aagattgtcc agacctacca
     2401 cgtcaatatg gcgggcacca acccctacac aaccatcacg cctcaggaga tcaatggcaa
     2461 atgggaccac gtgcggcagc tggtgcctcg gagggaccaa gctctgacgg aggagcatgc
     2521 ccgacagcag cacaatgaga ggctacgcaa gcagtttgga gcccaggcca atgtcatcgg
     2581 gccctggatc cagaccaaga tggaggagat cgggaggatc tccattgaga tgcatgggac
     2641 cctggaggac cagctcagcc acctgcggca gtatgagaag agcatcgtca actacaagcc
     2701 aaagattgat cagctggagg gcgaccacca gctcatccag gaggcgctca tcttcgacaa
     2761 caagcacacc aactacacca tggagcacat ccgtgtgggc tgggagcagc tgctcaccac
     2821 catcgccagg accatcaatg aggtagagaa ccagatcctg acccgggatg ccaagggcat
     2881 cagccaggag cagatgaatg agttccgggc ctccttcaac cactttgacc gggatcactc
     2941 cggcacactg ggtcccgagg agttcaaagc ctgcctcatc agcttgggtt atgatattgg
     3001 caacgacccc cagggagaag cagaatttgc ccgcatcatg agcattgtgg accccaaccg
     3061 cctgggggta gtgacattcc aggccttcat tgacttcatg tcccgcgaga cagccgacac
     3121 agatacagca gaccaagtca tggcttcctt caagatcctg gctggggaca agaactacat
     3181 taccatggac gagctgcgcc gcgagctgcc acccgaccag gctgagtact gcatcgcgcg
     3241 gatggccccc tacaccggcc ccgactccgt gccaggtgct ctggactaca tgtccttctc
     3301 cacggcgctg tacggcgaga gtgacctcaa gcttcgaatt ctgcagtcga cggtaccgcg
     3361 ggcccgggat ccaccggtcg ccaccatgag tgcgattaag ccagacatga agatcaaact
     3421 ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa
     3481 gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt
     3541 tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg ccaaatatcc
     3601 agacaacata caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag
     3661 cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga
     3721 cactttctat aataaagttc gattttatgg taccaacttt cccgccaatg gtccagttat
     3781 gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt
     3841 gctgacgggt gatattcata tggctttgtt gcttgaagga aatgcccatt accgatgtga
     3901 cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt
     3961 ggaccactgc attgagattt taagccatga caaagattac aacaaggtta agctgtatga
     4021 gcatgctgtt gctcattctg gattgcctga caatgccaga cgataagcgg ccgcgactct
     4081 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     4141 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     4201 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4261 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     4321 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     4381 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     4441 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     4501 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     4561 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4621 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4681 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4741 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     4801 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4861 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     4921 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4981 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5041 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5101 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5161 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5221 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     5281 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5341 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5401 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5461 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5521 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5581 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5641 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5701 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5761 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5821 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5881 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5941 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6001 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6061 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     6121 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     6181 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6241 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     6301 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     6361 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     6421 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     6481 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     6541 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6601 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6661 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6721 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6781 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6841 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6901 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6961 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7021 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7081 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7141 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7201 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7261 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7321 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7381 accg
//
