LOCUS       sequence-241219-       12368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ERBB4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12368)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ERBB4
FEATURES             Location/Qualifiers
     source          1..12368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7328..7352)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7360..7378
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7403..7422)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7404..7422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7428..7448)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7502..7543
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7564..8131
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7581..8133
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7725..7742)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7952..7971
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8145..8744
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8145..8164)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8641..8661
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8758..9346
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8811..8831)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9229..9240)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9421..9654
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9773..9791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9859..9963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9964..10032,10033..10824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10182..10201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10995..11583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11484..11503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11737..11754
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11994..12015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12030..12035,12036..12053,12054..12060)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12068..12084
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12073..12095
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12092..12108
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12092..12108
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12158..12177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg aagccggcga caggactttg ggtctgggtg
     3481 agccttctcg tggcggcggg gaccgtccag cccagcgatt ctcagtcagt gtgtgcagga
     3541 acggagaata aactgagctc tctctctgac ctggaacagc agtaccgagc cttgcgcaag
     3601 tactatgaaa actgtgaggt tgtcatgggc aacctggaga taaccagcat tgagcacaac
     3661 cgggacctct ccttcctgcg gtctgttcga gaagtcacag gctacgtgtt agtggctctt
     3721 aatcagtttc gttacctgcc tctggagaat ttacgcatta ttcgtgggac aaaactttat
     3781 gaggatcgat atgccttggc aatattttta aactacagaa aagatggaaa ctttggactt
     3841 caagaacttg gattaaagaa cttgacagaa atcctaaatg gtggagtcta tgtagaccag
     3901 aacaaattcc tttgttatgc agacaccatt cattggcaag atattgttcg gaacccatgg
     3961 ccttccaact tgactcttgt gtcaacaaat ggtagttcag gatgtggacg ttgccataag
     4021 tcctgtactg gccgttgctg gggacccaca gaaaatcatt gccagacttt gacaaggacg
     4081 gtgtgtgcag aacaatgtga cggcagatgc tacggacctt acgtcagtga ctgctgccat
     4141 cgagaatgtg ctggaggctg ctcaggacct aaggacacag actgctttgc ctgcatgaat
     4201 ttcaatgaca gtggagcatg tgttactcag tgtccccaaa cctttgtcta caatccaacc
     4261 acctttcaac tggagcacaa tttcaatgca aagtacacat atggagcatt ctgtgtcaag
     4321 aaatgtccac ataactttgt ggtagattcc agttcttgtg tgcgtgcctg ccctagttcc
     4381 aagatggaag tagaagaaaa tgggattaaa atgtgtaaac cttgcactga catttgccca
     4441 aaagcttgtg atggcattgg cacaggatca ttgatgtcag ctcagactgt ggattccagt
     4501 aacattgaca aattcataaa ctgtaccaag atcaatggga atttgatctt tctagtcact
     4561 ggtattcatg gggaccctta caatgcaatt gaagccatag acccagagaa actgaacgtc
     4621 tttcggacag tcagagagat aacaggtttc ctgaacatac agtcatggcc accaaacatg
     4681 actgacttca gtgttttttc taacctggtg accattggtg gaagagtact ctatagtggc
     4741 ctgtccttgc ttatcctcaa gcaacagggc atcacctctc tacagttcca gtccctgaag
     4801 gaaatcagcg caggaaacat ctatattact gacaacagca acctgtgtta ttatcatacc
     4861 attaactgga caacactctt cagcacaatc aaccagagaa tagtaatccg ggacaacaga
     4921 aaagctgaaa attgtactgc tgaaggaatg gtgtgcaacc atctgtgttc cagtgatggc
     4981 tgttggggac ctgggccaga ccaatgtctg tcgtgtcgcc gcttcagtag aggaaggatc
     5041 tgcatagagt cttgtaacct ctatgatggt gaatttcggg agtttgagaa tggctccatc
     5101 tgtgtggagt gtgaccccca gtgtgagaag atggaagatg gcctcctcac atgccatgga
     5161 ccgggtcctg acaactgtac aaagtgctct cattttaaag atggcccaaa ctgtgtggaa
     5221 aaatgtccag atggcttaca gggggcaaac agtttcattt tcaagtatgc tgatccagat
     5281 cgggagtgcc acccatgcca tccaaactgc acccaagggt gtaacggtcc cactagtcat
     5341 gactgcattt actacccatg gacgggccat tccactttac cacaacatgc tagaactccc
     5401 ctgattgcag ctggagtaat tggtgggctc ttcattctgg tcattgtggg tctgacattt
     5461 gctgtttatg ttagaaggaa gagcatcaaa aagaaaagag ccttgagaag attcttggaa
     5521 acagagttgg tggaaccatt aactcccagt ggcacagcac ccaatcaagc tcaacttcgt
     5581 attttgaaag aaactgagct gaagagggta aaagtccttg gctcaggtgc ttttggaacg
     5641 gtttataaag gtatttgggt acctgaagga gaaactgtga agattcctgt ggctattaag
     5701 attcttaatg agacaactgg tcccaaggca aatgtggagt tcatggatga agctctgatc
     5761 atggcaagta tggatcatcc acacctagtc cggttgctgg gtgtgtgtct gagcccaacc
     5821 atccagctgg ttactcaact tatgccccat ggctgcctgt tggagtatgt ccacgagcac
     5881 aaggataaca ttggatcaca actgctgctt aactggtgtg tccagatagc taagggaatg
     5941 atgtacctgg aagaaagacg actcgttcat cgggatttgg cagcccgtaa tgtcttagtg
     6001 aaatctccaa accatgtgaa aatcacagat tttgggctag ccagactctt ggaaggagat
     6061 gaaaaagagt acaatgctga tggaggaaag atgccaatta aatggatggc tctggagtgt
     6121 atacattaca ggaaattcac ccatcagagt gacgtttgga gctatggagt tactatatgg
     6181 gaactgatga cctttggagg aaaaccctat gatggaattc caacgcgaga aatccctgat
     6241 ttattagaga aaggagaacg tttgcctcag cctcccatct gcactattga cgtttacatg
     6301 gtcatggtca aatgttggat gattgatgct gacagtagac ctaaatttaa ggaactggct
     6361 gctgagtttt caaggatggc tcgagaccct caaagatacc tagttattca gggtgatgat
     6421 cgtatgaagc ttcccagtcc aaatgacagc aagttctttc agaatctctt ggatgaagag
     6481 gatttggaag atatgatgga tgctgaggag tacttggtcc ctcaggcttt caacatccca
     6541 cctcccatct atacttccag agcaagaatt gactcgaata ggaaccagtt tgtataccga
     6601 gatggaggtt ttgctgctga acaaggagtg tctgtgccct acagagcccc aactagcaca
     6661 attccagaag ctcctgtggc acagggtgct actgctgaga tttttgatga ctcctgctgt
     6721 aatggcaccc tacgcaagcc agtggcaccc catgtccaag aggacagtag cacccagagg
     6781 tacagtgctg accccaccgt gtttgcccca gaacggagcc cacgaggaga gctggatgag
     6841 gaaggttaca tgactcctat gcgagacaaa cccaaacaag aatacctgaa tccagtggag
     6901 gagaaccctt ttgtttctcg gagaaaaaat ggagaccttc aagcattgga taatcccgaa
     6961 tatcacaatg catccaatgg tccacccaag gccgaggatg agtatgtgaa tgagccactg
     7021 tacctcaaca cctttgccaa caccttggga aaagctgagt acctgaagaa caacatactg
     7081 tcaatgccag agaaggccaa gaaagcgttt gacaaccctg actactggaa ccacagcctg
     7141 ccacctcgga gcacccttca gcacccagac tacctgcagg agtacagcac aaaatatttt
     7201 tataaacaga atgggcggat ccggcctatt gtggcagaga atcctgaata cctctctgag
     7261 ttctccctga agccaggcac tgtgctgccg cctccacctt acagacaccg gaatactgtg
     7321 gtgtagaacc cagctttctt gtacaaagtg gtgatatcca attaaccctc actaaaggat
     7381 ttatgtagtt gagagtgata aaccctatag tgagtcgtat taccacccaa cttttctata
     7441 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     7501 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg
     7561 agatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     7621 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     7681 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     7741 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     7801 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     7861 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     7921 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     7981 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     8041 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     8101 cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatgacc gagtacaagc
     8161 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     8221 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     8281 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8341 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8401 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8461 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8521 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8581 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8641 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8701 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctaaatcgat agatcctaat
     8761 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8821 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8881 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8941 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     9001 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     9061 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     9121 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     9181 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9241 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     9301 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa
     9361 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     9421 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     9481 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9541 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9601 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     9661 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9721 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     9781 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     9841 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9901 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     9961 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    10021 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    10081 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    10141 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    10201 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    10261 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    10321 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    10381 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    10441 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    10501 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    10561 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    10621 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    10681 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    10741 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    10801 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    10861 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10921 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10981 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    11041 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    11101 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    11161 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    11221 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11281 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11341 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11401 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11461 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11521 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11581 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    11641 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    11701 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    11761 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    11821 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    11881 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
    11941 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    12001 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    12061 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
    12121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    12181 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    12241 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
    12301 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
    12361 ttagtagt
//
