LOCUS       sequence-241236-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-579-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-579-3p
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(112..136)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(364..383)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    429..484
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(483..502)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    868..893
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 gaatcaagga ggccgacaaa gagacatacg tcgagcagca cgaggtggct gtggccagat
       61 actgcgacct ccctagcaaa ctggggcaca aacttaattg agaattctac cacccagctt
      121 tcttgtacaa agtggtacgt ctcaagacca gaggtctctg cttactcgcg caatcgcggt
      181 ttataccaaa tgaaaatcgc ggtttatacc aaatgaaaat cgcggtttat accaaatgaa
      241 aatcgcggtt tataccaaat gaacaaccag aagagacctg tgcttcactc ctcaggtgca
      301 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
      361 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
      421 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
      481 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
      541 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
      601 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
      661 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
      721 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
      781 catagctgtc cctcttctct tatggagatc ccgagcggtg cgtttttatg cttgtagtat
      841 tgtataatgt ttttaagatc caagcttctg acctcttctc ttcctcccac agggcctcga
      901 gacaattgca tcgatggtac cagtgaaagt ctgcaatgaa ttcgagttgg cgcgcctgtc
      961 attctaa
//
