LOCUS       sequence-241239-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-652-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-652-3p
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(99..123)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(343..362)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    408..463
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(462..481)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    847..872
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 cgacaaagag acatacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
       61 tagcaaactg gggcacaaac ttaattgaga attctaccac ccagctttct tgtacaaagt
      121 ggtacgtctc aagaccagag gtctctgctt gctttaagca caaccctagt ggcgccattc
      181 acaaccctag tggcgccatt cacaacccta gtggcgccat tcacaaccct agtggcgcca
      241 ttcaaccaga agagacctgt gcttcactcc tcaggtgcag gctgcctatc agaaggtggt
      301 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
      361 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
      421 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
      481 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
      541 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
      601 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
      661 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
      721 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
      781 atggagatcc cgagcggtgc gtttttatgc ttgtagtatt gtataatgtt tttaagatcc
      841 aagcttctga cctcttctct tcctcccaca gggcctcgag acaattgcat cgatggtacc
      901 agtgaaagtc tgcaatgaat tcgagttggc gcgcctgtca ttctaaatct ctctttcagc
//
