LOCUS       sequence-241243-       12770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and
            NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, 
            PacI sites, sgRNA tail).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS
  TITLE     A CRISPR Interference Platform for Efficient Genetic Repression in 
            Candida albicans.
  JOURNAL   mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 
            10.1128/mSphere.00002-19.
  PUBMED    30760609
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2019-02-13"; volume: "4(1). pii: 4/1/e00002-19. doi"; pages: 
            " 10.1128/mSphere.00002-19"
COMMENT     Sequence Label: pRS159
FEATURES             Location/Qualifiers
     source          1..12770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     25..41
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        62..80
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             join(1114..1140,1141..1143,1144..3699,3700..3702,
                     3703..5217)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5230..5250
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(6544..6741)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(7342..7685)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(8648..8667)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8649..8667)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8674..8691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8674..8690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8683..8705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8919..8938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     misc_RNA        complement(8975..9049)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     10501..10522
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10685..10789
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10790..10858,10859..11650)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11008..11027)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11821..12409
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12310..12329
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12563..12580
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12697..12718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12733..12738,12739..12756,12757..12763)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg
       61 aaattaaccc tcactaaagg gaacaaaagc tggtaccaga gctattaaga tcaccagcct
      121 cgtttataat aaacttagtc taatatttct tttggaccaa aatagaaact gttctcaggt
      181 ggcaaaaaac agcttaaagt ctgtgttaaa aaaagatctc ggctatgcca atcaaaaagg
      241 agtgacatta ttatgtaaaa gaagaaatgt tgtaattccg cgaaacaaca attttaatgc
      301 ttactgtgcc gccaccaata tcctatggcc aaggggcctt taaacatgcc acggaaatat
      361 acagtcaaag taaacctact atgtctttag agccttcagg atcaattgtt tttaaatgaa
      421 tgactgcaaa actagaaaag tagcctctta cataagcaag tcatttgtac accgagagtt
      481 tagtaaccag tgaagcaagt atatctggca aacctgaaac ttatctgtga attataaagc
      541 tcataagtaa aaagtgtaga aaagaagata aggcagacca aatacaaatt tgcaacagta
      601 caacgaatat ttcagcgtca aaactagaga ataataaaga aaacgatctt ttcaaaaaga
      661 aaaaaccttt tagttttcct ttgttgttgt tgtgggtgtg tgctatttat attatatagt
      721 ttactcataa taccataaaa tattcggttt gattaggtta ttttaataag ctaatttgtt
      781 tctaatcgtg taatttatgc tgtgtatatt aagtagtgtg tgcactgccc aaaaatgttt
      841 gttgtttata gtcggttaaa gagaaaaaag aaaaaaagat ccatacacac acgttaatta
      901 gttgttcaac gtaatacact catattttgt tcttatttgc tttcggtcgc tgttctcacc
      961 aagatttatt gccaacgaaa caattttttt ttatatattt tcagattttt ctttttttcc
     1021 tttcctttcc ttttctaatt ttcactcctg gttttctttc tttcttagaa acattatctc
     1081 gatattaata ttaaaaaaat ataatcattc aaaatggata aaaaatactc cataggtttg
     1141 gccattggaa ccaattcagt tggatgggct gtaatcactg atgaatataa ggttccttct
     1201 aaaaagttca aagttttagg aaatactgat agacactcca ttaaaaagaa tttaattggt
     1261 gccttgttgt ttgacagtgg tgaaacagct gaagcaactc gtttgaaaag aacagctaga
     1321 agaagatata ccagaagaaa gaacagaatt tgttacttgc aagaaatctt ttctaatgaa
     1381 atggcaaagg ttgatgattc tttttttcat agattggaag aaagtttctt agttgaagaa
     1441 gataagaaac atgaaagaca tccaatcttt ggtaatattg tagatgaagt ggcttaccac
     1501 gaaaaatatc ctacaattta tcatttgaga aaaaaattag ttgactccac tgataaagct
     1561 gacttaagac ttatatattt ggcattggct cacatgatca agttcagagg tcacttctta
     1621 attgaaggtg atttgaatcc agataattcc gatgtcgaca agttgtttat tcaattggtt
     1681 caaacttaca accaactttt cgaggaaaat ccaatcaatg cctcaggtgt ggatgctaaa
     1741 gctattttat ctgctagatt gtcaaaaagt agacgtttgg aaaatcttat tgcccaattg
     1801 ccaggtgaaa aaaaaaatgg attgtttgga aacttaattg cattatcttt gggtttgacc
     1861 ccaaatttca aatcaaactt tgatttggcc gaggatgcta aacttcaatt gtctaaggat
     1921 acatacgatg acgatttaga taatcttctt gcccaaattg gtgatcaata tgcagattta
     1981 ttcttggcag ctaagaactt gagtgatgca attttgttaa gtgacatttt gagagtcaac
     2041 acagaaatta caaaagctcc tttatccgcc tctatgatta aaagatatga tgaacaccat
     2101 caagatttaa ccttgttgaa agctcttgta cgtcaacaat taccagaaaa gtataaagaa
     2161 atattctttg atcaatctaa aaatggttac gctggttata ttgatggtgg tgctagtcaa
     2221 gaagaatttt ataagtttat caagccaatt cttgaaaaaa tggatggtac tgaggaattg
     2281 ttggttaagt tgaatcgaga ggatttactt cgtaagcaaa gaacatttga taacggttct
     2341 atccctcatc aaatccactt aggtgagtta cacgctatct tgagacgtca ggaagatttt
     2401 tacccttttt tgaaagacaa tcgtgaaaaa attgaaaaaa ttttgacttt cagaattcct
     2461 tattacgtcg ggccattggc tagaggtaat tctagatttg catggatgac tcgtaaatct
     2521 gaagagacta tcaccccatg gaattttgag gaagttgttg ataagggagc ctccgctcaa
     2581 tcattcatcg agagaatgac caatttcgac aaaaacttgc caaatgagaa ggtgttacct
     2641 aaacattctt tgttatatga atatttcact gtttataatg aattaacaaa agtcaaatac
     2701 gtcactgaag gtatgagaaa accagcattc ttaagtggtg aacaaaagaa agctatcgtt
     2761 gacttgttat tcaaaactaa tcgtaaagtc actgtcaaac aacttaaaga ggactatttt
     2821 aagaaaatag agtgtttcga ttccgttgaa atcagtgggg ttgaggatag atttaatgct
     2881 agtttaggaa cttatcacga tttgttgaaa attatcaaag acaaagattt tttggataat
     2941 gaagaaaacg aagatatctt ggaggacatt gtattgactt tgactttgtt tgaggataga
     3001 gaaatgatcg aagaacgttt aaaaacttac gctcacttat tcgacgataa agtcatgaaa
     3061 caattaaaaa gaagaagata taccggttgg ggaagattat ctagaaagtt gattaatggt
     3121 attagagata agcaaagtgg taaaaccatc ttagatttcc ttaaaagtga tggttttgca
     3181 aacagaaact ttatgcagtt gattcatgat gatagtttga cctttaagga agatattcaa
     3241 aaagctcagg tttctggaca gggtgattca ttgcatgaac atattgccaa cttagcaggg
     3301 tctccagcta tcaaaaaggg aattttacaa actgttaaag ttgttgatga gttggtgaaa
     3361 gttatgggac gtcataagcc tgaaaatata gttatagaaa tggctagaga aaatcaaaca
     3421 actcagaaag gacaaaagaa ttctcgtgaa agaatgaaaa gaatagaaga aggaattaaa
     3481 gagttgggtt ctcaaatctt aaaagaacac ccagtcgaaa acacccagtt acagaacgag
     3541 aagctttact tgtactattt gcaaaacggg cgtgacatgt atgtagatca agagttggat
     3601 atcaatagac tttcagatta cgatgtcgat cacatagttc ctcagtcatt tttaaaagat
     3661 gattccattg acaacaaagt tttaacaaga tctgataagg ctagaggtaa atcagacaat
     3721 gtgccatcag aagaagtggt taagaaaatg aagaactatt ggagacaatt gttaaatgca
     3781 aagttgatta ctcagagaaa atttgacaac ttaactaaag ctgaaagagg aggattatcc
     3841 gaattagata aagctggatt cataaaacgt cagttggttg aaacaagaca aattactaaa
     3901 catgtagccc aaattttaga ttcccgaatg aatactaaat atgatgaaaa tgacaaatta
     3961 atcagagagg tcaaagtgat cacattaaag tcaaagttgg tgtcagattt ccgaaaggat
     4021 tttcaattct acaaggtgag agagatcaac aattatcatc atgctcacga cgcatatttg
     4081 aatgcagtgg taggaactgc cttaataaag aagtatccta agttagaatc cgaattcgtg
     4141 tatggtgatt ataaagtcta cgatgtcaga aagatgatag caaaatcaga acaagaaatt
     4201 gggaaagcca ccgcaaagta tttcttttac tccaacatca tgaatttctt taagaccgaa
     4261 atcaccttgg ctaacgggga aatcagaaaa cgtcctttga ttgaaacaaa tggagaaacc
     4321 ggtgaaattg tgtgggataa ggggagagat tttgctactg ttagaaaagt tttatcaatg
     4381 cctcaagtta atattgtaaa gaagactgaa gttcaaactg gtggtttctc taaggaatcc
     4441 attcttccaa aacgaaactc agacaagttg atcgcccgaa aaaaagattg ggatcctaag
     4501 aaatacggag gttttgattc tcctactgtc gcatattctg tcttggtcgt ggctaaagtt
     4561 gaaaagggta aatccaaaaa attgaaatct gtgaaagaat tgttaggtat taccatcatg
     4621 gagcgttcat cctttgagaa aaacccaatt gactttctcg aagcgaaggg ctataaagaa
     4681 gtgaagaaag atttgattat taagttgcca aagtattctc tctttgaact tgaaaatggg
     4741 agaaagagaa tgttggcttc agctggagaa ttgcaaaaag gaaacgagtt agctttgcct
     4801 tcaaagtacg ttaatttctt atacttagca tctcattatg aaaagttgaa ggggtcccct
     4861 gaagacaatg agcaaaagca attattcgta gaacaacata aacactattt ggatgagata
     4921 attgaacaaa tctccgagtt ttctaaaaga gttattttgg ctgatgcaaa tttggataaa
     4981 gttttatctg cttataataa gcatcgtgac aaaccaattc gagaacaagc tgaaaatatt
     5041 atccacttgt tcacattgac taacttggga gctccagccg cgtttaagta ctttgacacc
     5101 accattgacc gtaagagata tacttcaaca aaagaagtgt tagacgccac attgattcat
     5161 cagtccataa ctggtttata tgaaactaga atcgatttgt cacaattggg gggtgatagc
     5221 cgagcggatc ctaagaaaaa acgaaaagtc agtggtggtg gtagtgggtc cgggtcagga
     5281 ggttcatcca agttaggagg ttcaggaggg agtatggaac gagttagaat gatcaacgta
     5341 cagcgtttgt tagaggctgc agagtttttg gaaagacgag aacgagaatg cgagcatggt
     5401 tacgcatctt ccttcccatc tatgccatcc ccacgaggga gtggaggggg gtctgggtcc
     5461 ggttctggat ctgggagttc aaaattaggg ggatccgggg gatctatgga acgtgtccgt
     5521 atgattaatg tccaaagatt attagaagcc gcagaatttt tagaaagacg agaaagagaa
     5581 tgtgaacatg gttatgcatc atcatttcca agtatgcctt caccaagagg ttaacccggg
     5641 ggatccgagt gaaattctgg aaatctggaa atctggtttt gtattcttgt tattcttctt
     5701 tttgttatta catatataac ttgttacttt tttaaaaaaa tctttgttta ttttataaat
     5761 atataaaact aaatttaaga agaagagaaa aatgttttat ttgagagatt gaaattttac
     5821 ttgaatttag cttagctttt ataaagtatt attatgtaaa aaaacaaaac aaatatacat
     5881 taaaaagtta agactataaa atagccaccc aaggcatttc tatatcttgt tgttgttgtt
     5941 ttcatcttct gtatcagagg aacttatttt attattttcg tcacgggtat tttctcttgt
     6001 ttgatgattc atcccattca ttccatcata aaattctaga aggaccacct ttgattgtaa
     6061 atagtaataa ttaccaccct tatttaatta tttatttaac ttatttattt atttattata
     6121 catatataca aatctaataa agtgaaaatc tcccccttca catttataat tggccagttt
     6181 ttttcaaata agcattccaa ccagcatctc tatacctttt accttcaata tctggatctc
     6241 ttcctttacc aaacaatcct ctaccaacaa tgataatatc agttccagtg ctaacaactt
     6301 catcaacagt tctatattgt tgtcctaatc catcaccttt atcatctaat ccaactccag
     6361 gtgtcataat aagccaatca aatccttctt cttgtccacc catatcacgt tgggcaataa
     6421 atccaataac aaattcctta tcggatttag caatttcaac agttttttga gaatattctc
     6481 catacgaatt cgagctcgtt taaacgtcga cactggatgg cggcgttagt atcgaatcga
     6541 cagcagtata gcgaccagca ttcacatacg attgacgcat gatattactt tctgcgcact
     6601 taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa atataaatca cgctaacatt
     6661 tgattaaaat agaacaacta caatataaaa aaactataca aatgacaagt tcttgaaaac
     6721 aagaatcttt ttattgtcag tactgactcg agttattatg gacatggcat agacatatac
     6781 aaagcttgtt caccatcgga agcagtacca tcgtataaag cagtatccaa accacacaaa
     6841 gtgaaaccca ttcttctata agcatgaata gctggagcat taacattggt aacttccaac
     6901 cacaaatgac cagcacctct ttctctggcg aattcagtag ccaaacccat caaagctcta
     6961 ccaacaccat gacctctatg ttctggagca acttcaatat cttcaacagt caatcttctg
     7021 ttccaaccag aataagaaac aacaacgaaa ccagccaaat caccatcatc accataagca
     7081 acgaaagttc tagaatctgg atcaccatct tcaccagcat cggattcatc atcggattca
     7141 tcatctggga aaaccttagt caatggtgga tcaactggaa cttctctcaa agtgaaacca
     7201 tcaccagtag cagtaactct aaaaacagta tcggtagtga aagaaccatc caaagcttca
     7261 atagcttcag catcacctgg aacagaagtt ctgtatctat aagcagtatc atccaaagta
     7321 gtagacataa ttgtaggatc cggttgttta tgttcggatg tgatgtgaga actgtatcct
     7381 agcaagattt taaaaggaag tatatgaaag aagaacctca gtggcaaatc ctaacctttt
     7441 atatttctct acaggggcgc ggcgtgggga caattcaacg cgtctgtgag gggagcgttt
     7501 ccctgctcgc aggtctgcag cgaggagccg taatttttgc ttcgcgccgt gcggccatca
     7561 aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg gctaaatgta cgggcgacag
     7621 tcacatcatg cccctgagct gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc
     7681 atgtcgctgg ccgggtgacc cggcggggac gaggcaagct tgatatgcta atgatcccac
     7741 tgatgataat tcagctaaca tcaataaccc tcttggctct tggttggtgg tggtttcttt
     7801 agctccctgt tttaatcctt caactactcc attcccagtg acaccatgag cattagtaat
     7861 atctgcccaa ctactaattt tataaactcc accaatatat tgtttcttca cggtattacc
     7921 aatatcagca aattttctat cttcaaaaat cataaattga tgtttacgtg aaagttctaa
     7981 taatggttca atagtggatt cataggaaaa atcattgatt atatcaatat gagtcttgat
     8041 taagcataca taaggaccca atttatcaat taattcaagg aattccttag tggtatcaac
     8101 atcaattgat gcacataaat tggttttctt cagttccatt aatcgaaata atcgttgtgc
     8161 tactggtgag gcatgagttt ctgctctctc actataggtc ttagtgttga ctgtcatatc
     8221 ttttgtttgc tatttctatt tctatttctt tcttcaactg taaaaggggc gatatataaa
     8281 ccagtgtgta tggggttgtt gctcaggaat tgatcaatgt ttaaaagaat ggttgaaatc
     8341 atcattgaag aacaaaaaaa aagatttttt ctttcattat gttatctgta taatatgcag
     8401 gaaataagat tgcaaaaaaa tagccgtttt tttggatact atcaaacaag aggaatgact
     8461 aatacagaat tagtgttacg aatcaatggc actacagcaa ctttcaacaa aataatctac
     8521 gttcaaaata taatatatac tattaaataa aatcaaataa taaaataatt ttaaagaaag
     8581 tagtaccagc tctttttttt gtttccgttt ataccatcca aatcaattcc tattagtctc
     8641 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     8701 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8761 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     8821 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8881 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8941 ctttctcgcc acgttcgccg gcaaaaaaaa gcaccaccga ctcggtgcca ctttttcaag
     9001 ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaact gaagagccat
     9061 taatgctctt cctcgaaatt aaaaatagtt tacgcaagtc tcgaacttcc accacaattt
     9121 aattgtaaga acgctgcgaa aacatattct ccaatcctat tgggccgaag tttcagctat
     9181 gccaaaggca tcaaattttt ttttataaaa ttttccactt ttccatacca gtgaaattat
     9241 cacgtgatgg agtgagtatc tataatattt gattgtttct ttgatgaggc aatactcgag
     9301 tatagttatt gaccaatgac ttttaccggc taaacaagtg gtattcaagc acaattcagt
     9361 tttctggatc tctaataatt gcaattgcaa ttgcttcaca tactctgttt ataattcagt
     9421 ttctttctct tgatgttact gatatattaa gttaataaat accaataact ttttttcaaa
     9481 aatcttctat tttcctttca attgattatt taaacacgaa tcgttaataa gctgtgattg
     9541 ctttttctct ttctttttct tttctttgtt caagtttgaa aaaaaaaaaa aaaggtttct
     9601 cttgttcgat gtagaattca gtgggttata gttgtaatga tatatggaat aaaaagaaag
     9661 aaagagaaga gaaagagaag aagagaagaa ttaagtaaac gtgtattaga atttaaaaat
     9721 taaaattcat gataatcttt aataataatt aactaagaat cacaaaattg aaaatttgaa
     9781 agaaaaaaga tcactccaag gtcgtgtcaa gtgactagaa attcttaatt aaaatagtta
     9841 taacaaaaaa agaaatttgt atataacaat taagtaaaag aaaaaaaagc aattcgcttt
     9901 gtataataat acccagaaaa atatattcat atattcataa atcaaacatg aaatgaagaa
     9961 tgctgaatca cttgatagga tttagttcca ttatggtact aagaagtaat attacttcta
    10021 tgaaaaggtt gaatccgaga tcaaaattca tcttttcata tactctggag aaaaaaaaaa
    10081 aagtaattgt agtaagaatg acaagtatca gttgagaaaa aaagtaacaa agagaaagct
    10141 cggaggaggc tccccaaagg ttttatcacc aatggtggta ccactaaccc aagaacagaa
    10201 aaagcattga tattttgaaa aatacaatac tcatctttta acccaaacct tttcttgtcc
    10261 tttaaaactt ttatctcatg tctgtccaaa gtcatattgt cttactactt tctctccttc
    10321 aacgtccttc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    10381 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    10441 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    10501 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    10561 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    10621 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
    10681 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    10741 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    10801 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    10861 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    10921 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    10981 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
    11041 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    11101 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    11161 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    11221 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    11281 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    11341 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    11401 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    11461 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    11521 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    11581 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    11641 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    11701 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    11761 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    11821 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    11881 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    11941 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    12001 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    12061 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    12121 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    12181 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    12241 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    12301 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    12361 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    12421 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    12481 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    12541 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcgtaag agcgcccaat
    12601 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    12661 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    12721 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
//
