LOCUS       sequence-241246-       12767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains 
            dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 
            bp upstream of HSP90 start codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12767)
  AUTHORS   Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS
  TITLE     A CRISPR Interference Platform for Efficient Genetic Repression in 
            Candida albicans.
  JOURNAL   mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 
            10.1128/mSphere.00002-19.
  PUBMED    30760609
REFERENCE   2  (bases 1 to 12767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2019-02-13"; volume: "4(1). pii: 4/1/e00002-19. doi"; pages: 
            " 10.1128/mSphere.00002-19"
COMMENT     Sequence Label: pRS263
FEATURES             Location/Qualifiers
     source          1..12767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..66
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..79
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        100..118
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             join(1152..1178,1179..1181,1182..3737,3738..3740,
                     3741..5255)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5268..5288
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(6582..6779)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(7380..7723)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(8686..8705)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8687..8705)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8712..8729)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8712..8728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8721..8743)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8957..8976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     misc_RNA        complement(9013..9087)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     10536..10557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10720..10824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10825..10893,10894..11685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11043..11062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11856..12444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12345..12364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12598..12615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12732..12753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga
      121 acaaaagctg gtaccagagc tattaagatc accagcctcg tttataataa acttagtcta
      181 atatttcttt tggaccaaaa tagaaactgt tctcaggtgg caaaaaacag cttaaagtct
      241 gtgttaaaaa aagatctcgg ctatgccaat caaaaaggag tgacattatt atgtaaaaga
      301 agaaatgttg taattccgcg aaacaacaat tttaatgctt actgtgccgc caccaatatc
      361 ctatggccaa ggggccttta aacatgccac ggaaatatac agtcaaagta aacctactat
      421 gtctttagag ccttcaggat caattgtttt taaatgaatg actgcaaaac tagaaaagta
      481 gcctcttaca taagcaagtc atttgtacac cgagagttta gtaaccagtg aagcaagtat
      541 atctggcaaa cctgaaactt atctgtgaat tataaagctc ataagtaaaa agtgtagaaa
      601 agaagataag gcagaccaaa tacaaatttg caacagtaca acgaatattt cagcgtcaaa
      661 actagagaat aataaagaaa acgatctttt caaaaagaaa aaacctttta gttttccttt
      721 gttgttgttg tgggtgtgtg ctatttatat tatatagttt actcataata ccataaaata
      781 ttcggtttga ttaggttatt ttaataagct aatttgtttc taatcgtgta atttatgctg
      841 tgtatattaa gtagtgtgtg cactgcccaa aaatgtttgt tgtttatagt cggttaaaga
      901 gaaaaaagaa aaaaagatcc atacacacac gttaattagt tgttcaacgt aatacactca
      961 tattttgttc ttatttgctt tcggtcgctg ttctcaccaa gatttattgc caacgaaaca
     1021 attttttttt atatattttc agatttttct ttttttcctt tcctttcctt ttctaatttt
     1081 cactcctggt tttctttctt tcttagaaac attatctcga tattaatatt aaaaaaatat
     1141 aatcattcaa aatggataaa aaatactcca taggtttggc cattggaacc aattcagttg
     1201 gatgggctgt aatcactgat gaatataagg ttccttctaa aaagttcaaa gttttaggaa
     1261 atactgatag acactccatt aaaaagaatt taattggtgc cttgttgttt gacagtggtg
     1321 aaacagctga agcaactcgt ttgaaaagaa cagctagaag aagatatacc agaagaaaga
     1381 acagaatttg ttacttgcaa gaaatctttt ctaatgaaat ggcaaaggtt gatgattctt
     1441 tttttcatag attggaagaa agtttcttag ttgaagaaga taagaaacat gaaagacatc
     1501 caatctttgg taatattgta gatgaagtgg cttaccacga aaaatatcct acaatttatc
     1561 atttgagaaa aaaattagtt gactccactg ataaagctga cttaagactt atatatttgg
     1621 cattggctca catgatcaag ttcagaggtc acttcttaat tgaaggtgat ttgaatccag
     1681 ataattccga tgtcgacaag ttgtttattc aattggttca aacttacaac caacttttcg
     1741 aggaaaatcc aatcaatgcc tcaggtgtgg atgctaaagc tattttatct gctagattgt
     1801 caaaaagtag acgtttggaa aatcttattg cccaattgcc aggtgaaaaa aaaaatggat
     1861 tgtttggaaa cttaattgca ttatctttgg gtttgacccc aaatttcaaa tcaaactttg
     1921 atttggccga ggatgctaaa cttcaattgt ctaaggatac atacgatgac gatttagata
     1981 atcttcttgc ccaaattggt gatcaatatg cagatttatt cttggcagct aagaacttga
     2041 gtgatgcaat tttgttaagt gacattttga gagtcaacac agaaattaca aaagctcctt
     2101 tatccgcctc tatgattaaa agatatgatg aacaccatca agatttaacc ttgttgaaag
     2161 ctcttgtacg tcaacaatta ccagaaaagt ataaagaaat attctttgat caatctaaaa
     2221 atggttacgc tggttatatt gatggtggtg ctagtcaaga agaattttat aagtttatca
     2281 agccaattct tgaaaaaatg gatggtactg aggaattgtt ggttaagttg aatcgagagg
     2341 atttacttcg taagcaaaga acatttgata acggttctat ccctcatcaa atccacttag
     2401 gtgagttaca cgctatcttg agacgtcagg aagattttta cccttttttg aaagacaatc
     2461 gtgaaaaaat tgaaaaaatt ttgactttca gaattcctta ttacgtcggg ccattggcta
     2521 gaggtaattc tagatttgca tggatgactc gtaaatctga agagactatc accccatgga
     2581 attttgagga agttgttgat aagggagcct ccgctcaatc attcatcgag agaatgacca
     2641 atttcgacaa aaacttgcca aatgagaagg tgttacctaa acattctttg ttatatgaat
     2701 atttcactgt ttataatgaa ttaacaaaag tcaaatacgt cactgaaggt atgagaaaac
     2761 cagcattctt aagtggtgaa caaaagaaag ctatcgttga cttgttattc aaaactaatc
     2821 gtaaagtcac tgtcaaacaa cttaaagagg actattttaa gaaaatagag tgtttcgatt
     2881 ccgttgaaat cagtggggtt gaggatagat ttaatgctag tttaggaact tatcacgatt
     2941 tgttgaaaat tatcaaagac aaagattttt tggataatga agaaaacgaa gatatcttgg
     3001 aggacattgt attgactttg actttgtttg aggatagaga aatgatcgaa gaacgtttaa
     3061 aaacttacgc tcacttattc gacgataaag tcatgaaaca attaaaaaga agaagatata
     3121 ccggttgggg aagattatct agaaagttga ttaatggtat tagagataag caaagtggta
     3181 aaaccatctt agatttcctt aaaagtgatg gttttgcaaa cagaaacttt atgcagttga
     3241 ttcatgatga tagtttgacc tttaaggaag atattcaaaa agctcaggtt tctggacagg
     3301 gtgattcatt gcatgaacat attgccaact tagcagggtc tccagctatc aaaaagggaa
     3361 ttttacaaac tgttaaagtt gttgatgagt tggtgaaagt tatgggacgt cataagcctg
     3421 aaaatatagt tatagaaatg gctagagaaa atcaaacaac tcagaaagga caaaagaatt
     3481 ctcgtgaaag aatgaaaaga atagaagaag gaattaaaga gttgggttct caaatcttaa
     3541 aagaacaccc agtcgaaaac acccagttac agaacgagaa gctttacttg tactatttgc
     3601 aaaacgggcg tgacatgtat gtagatcaag agttggatat caatagactt tcagattacg
     3661 atgtcgatca catagttcct cagtcatttt taaaagatga ttccattgac aacaaagttt
     3721 taacaagatc tgataaggct agaggtaaat cagacaatgt gccatcagaa gaagtggtta
     3781 agaaaatgaa gaactattgg agacaattgt taaatgcaaa gttgattact cagagaaaat
     3841 ttgacaactt aactaaagct gaaagaggag gattatccga attagataaa gctggattca
     3901 taaaacgtca gttggttgaa acaagacaaa ttactaaaca tgtagcccaa attttagatt
     3961 cccgaatgaa tactaaatat gatgaaaatg acaaattaat cagagaggtc aaagtgatca
     4021 cattaaagtc aaagttggtg tcagatttcc gaaaggattt tcaattctac aaggtgagag
     4081 agatcaacaa ttatcatcat gctcacgacg catatttgaa tgcagtggta ggaactgcct
     4141 taataaagaa gtatcctaag ttagaatccg aattcgtgta tggtgattat aaagtctacg
     4201 atgtcagaaa gatgatagca aaatcagaac aagaaattgg gaaagccacc gcaaagtatt
     4261 tcttttactc caacatcatg aatttcttta agaccgaaat caccttggct aacggggaaa
     4321 tcagaaaacg tcctttgatt gaaacaaatg gagaaaccgg tgaaattgtg tgggataagg
     4381 ggagagattt tgctactgtt agaaaagttt tatcaatgcc tcaagttaat attgtaaaga
     4441 agactgaagt tcaaactggt ggtttctcta aggaatccat tcttccaaaa cgaaactcag
     4501 acaagttgat cgcccgaaaa aaagattggg atcctaagaa atacggaggt tttgattctc
     4561 ctactgtcgc atattctgtc ttggtcgtgg ctaaagttga aaagggtaaa tccaaaaaat
     4621 tgaaatctgt gaaagaattg ttaggtatta ccatcatgga gcgttcatcc tttgagaaaa
     4681 acccaattga ctttctcgaa gcgaagggct ataaagaagt gaagaaagat ttgattatta
     4741 agttgccaaa gtattctctc tttgaacttg aaaatgggag aaagagaatg ttggcttcag
     4801 ctggagaatt gcaaaaagga aacgagttag ctttgccttc aaagtacgtt aatttcttat
     4861 acttagcatc tcattatgaa aagttgaagg ggtcccctga agacaatgag caaaagcaat
     4921 tattcgtaga acaacataaa cactatttgg atgagataat tgaacaaatc tccgagtttt
     4981 ctaaaagagt tattttggct gatgcaaatt tggataaagt tttatctgct tataataagc
     5041 atcgtgacaa accaattcga gaacaagctg aaaatattat ccacttgttc acattgacta
     5101 acttgggagc tccagccgcg tttaagtact ttgacaccac cattgaccgt aagagatata
     5161 cttcaacaaa agaagtgtta gacgccacat tgattcatca gtccataact ggtttatatg
     5221 aaactagaat cgatttgtca caattggggg gtgatagccg agcggatcct aagaaaaaac
     5281 gaaaagtcag tggtggtggt agtgggtccg ggtcaggagg ttcatccaag ttaggaggtt
     5341 caggagggag tatggaacga gttagaatga tcaacgtaca gcgtttgtta gaggctgcag
     5401 agtttttgga aagacgagaa cgagaatgcg agcatggtta cgcatcttcc ttcccatcta
     5461 tgccatcccc acgagggagt ggaggggggt ctgggtccgg ttctggatct gggagttcaa
     5521 aattaggggg atccggggga tctatggaac gtgtccgtat gattaatgtc caaagattat
     5581 tagaagccgc agaattttta gaaagacgag aaagagaatg tgaacatggt tatgcatcat
     5641 catttccaag tatgccttca ccaagaggtt aacccggggg atccgagtga aattctggaa
     5701 atctggaaat ctggttttgt attcttgtta ttcttctttt tgttattaca tatataactt
     5761 gttacttttt taaaaaaatc tttgtttatt ttataaatat ataaaactaa atttaagaag
     5821 aagagaaaaa tgttttattt gagagattga aattttactt gaatttagct tagcttttat
     5881 aaagtattat tatgtaaaaa aacaaaacaa atatacatta aaaagttaag actataaaat
     5941 agccacccaa ggcatttcta tatcttgttg ttgttgtttt catcttctgt atcagaggaa
     6001 cttattttat tattttcgtc acgggtattt tctcttgttt gatgattcat cccattcatt
     6061 ccatcataaa attctagaag gaccaccttt gattgtaaat agtaataatt accaccctta
     6121 tttaattatt tatttaactt atttatttat ttattataca tatatacaaa tctaataaag
     6181 tgaaaatctc ccccttcaca tttataattg gccagttttt ttcaaataag cattccaacc
     6241 agcatctcta taccttttac cttcaatatc tggatctctt cctttaccaa acaatcctct
     6301 accaacaatg ataatatcag ttccagtgct aacaacttca tcaacagttc tatattgttg
     6361 tcctaatcca tcacctttat catctaatcc aactccaggt gtcataataa gccaatcaaa
     6421 tccttcttct tgtccaccca tatcacgttg ggcaataaat ccaataacaa attccttatc
     6481 ggatttagca atttcaacag ttttttgaga atattctcca tacgaattcg agctcgttta
     6541 aacgtcgaca ctggatggcg gcgttagtat cgaatcgaca gcagtatagc gaccagcatt
     6601 cacatacgat tgacgcatga tattactttc tgcgcactta acttcgcatc tgggcagatg
     6661 atgtcgaggc gaaaaaaaat ataaatcacg ctaacatttg attaaaatag aacaactaca
     6721 atataaaaaa actatacaaa tgacaagttc ttgaaaacaa gaatcttttt attgtcagta
     6781 ctgactcgag ttattatgga catggcatag acatatacaa agcttgttca ccatcggaag
     6841 cagtaccatc gtataaagca gtatccaaac cacacaaagt gaaacccatt cttctataag
     6901 catgaatagc tggagcatta acattggtaa cttccaacca caaatgacca gcacctcttt
     6961 ctctggcgaa ttcagtagcc aaacccatca aagctctacc aacaccatga cctctatgtt
     7021 ctggagcaac ttcaatatct tcaacagtca atcttctgtt ccaaccagaa taagaaacaa
     7081 caacgaaacc agccaaatca ccatcatcac cataagcaac gaaagttcta gaatctggat
     7141 caccatcttc accagcatcg gattcatcat cggattcatc atctgggaaa accttagtca
     7201 atggtggatc aactggaact tctctcaaag tgaaaccatc accagtagca gtaactctaa
     7261 aaacagtatc ggtagtgaaa gaaccatcca aagcttcaat agcttcagca tcacctggaa
     7321 cagaagttct gtatctataa gcagtatcat ccaaagtagt agacataatt gtaggatccg
     7381 gttgtttatg ttcggatgtg atgtgagaac tgtatcctag caagatttta aaaggaagta
     7441 tatgaaagaa gaacctcagt ggcaaatcct aaccttttat atttctctac aggggcgcgg
     7501 cgtggggaca attcaacgcg tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg
     7561 aggagccgta atttttgctt cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt
     7621 atacatgggg atgtatgggc taaatgtacg ggcgacagtc acatcatgcc cctgagctgc
     7681 gcacgtcaag actgtcaagg agggtattct gggcctccat gtcgctggcc gggtgacccg
     7741 gcggggacga ggcaagcttg atatgctaat gatcccactg atgataattc agctaacatc
     7801 aataaccctc ttggctcttg gttggtggtg gtttctttag ctccctgttt taatccttca
     7861 actactccat tcccagtgac accatgagca ttagtaatat ctgcccaact actaatttta
     7921 taaactccac caatatattg tttcttcacg gtattaccaa tatcagcaaa ttttctatct
     7981 tcaaaaatca taaattgatg tttacgtgaa agttctaata atggttcaat agtggattca
     8041 taggaaaaat cattgattat atcaatatga gtcttgatta agcatacata aggacccaat
     8101 ttatcaatta attcaaggaa ttccttagtg gtatcaacat caattgatgc acataaattg
     8161 gttttcttca gttccattaa tcgaaataat cgttgtgcta ctggtgaggc atgagtttct
     8221 gctctctcac tataggtctt agtgttgact gtcatatctt ttgtttgcta tttctatttc
     8281 tatttctttc ttcaactgta aaaggggcga tatataaacc agtgtgtatg gggttgttgc
     8341 tcaggaattg atcaatgttt aaaagaatgg ttgaaatcat cattgaagaa caaaaaaaaa
     8401 gattttttct ttcattatgt tatctgtata atatgcagga aataagattg caaaaaaata
     8461 gccgtttttt tggatactat caaacaagag gaatgactaa tacagaatta gtgttacgaa
     8521 tcaatggcac tacagcaact ttcaacaaaa taatctacgt tcaaaatata atatatacta
     8581 ttaaataaaa tcaaataata aaataatttt aaagaaagta gtaccagctc ttttttttgt
     8641 ttccgtttat accatccaaa tcaattccta ttagtctcca attcgcccta tagtgagtcg
     8701 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8761 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8821 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8881 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8941 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9001 aaaaaaaagc accaccgact cggtgccact ttttcaagtt gataacggac tagccttatt
     9061 ttaacttgct atttctagct ctaaaacacg gagaccactg gaaaaattcg aaattaaaaa
     9121 tagtttacgc aagtctcgaa cttccaccac aatttaattg taagaacgct gcgaaaacat
     9181 attctccaat cctattgggc cgaagtttca gctatgccaa aggcatcaaa ttttttttta
     9241 taaaattttc cacttttcca taccagtgaa attatcacgt gatggagtga gtatctataa
     9301 tatttgattg tttctttgat gaggcaatac tcgagtatag ttattgacca atgactttta
     9361 ccggctaaac aagtggtatt caagcacaat tcagttttct ggatctctaa taattgcaat
     9421 tgcaattgct tcacatactc tgtttataat tcagtttctt tctcttgatg ttactgatat
     9481 attaagttaa taaataccaa taactttttt tcaaaaatct tctattttcc tttcaattga
     9541 ttatttaaac acgaatcgtt aataagctgt gattgctttt tctctttctt tttcttttct
     9601 ttgttcaagt ttgaaaaaaa aaaaaaaagg tttctcttgt tcgatgtaga attcagtggg
     9661 ttatagttgt aatgatatat ggaataaaaa gaaagaaaga gaagagaaag agaagaagag
     9721 aagaattaag taaacgtgta ttagaattta aaaattaaaa ttcatgataa tctttaataa
     9781 taattaacta agaatcacaa aattgaaaat ttgaaagaaa aaagatcact ccaaggtcgt
     9841 gtcaagtgac tagaaattct taattaaaat agttataaca aaaaaagaaa tttgtatata
     9901 acaattaagt aaaagaaaaa aaagcaattc gctttgtata ataataccca gaaaaatata
     9961 ttcatatatt cataaatcaa acatgaaatg aagaatgctg aatcacttga taggatttag
    10021 ttccattatg gtactaagaa gtaatattac ttctatgaaa aggttgaatc cgagatcaaa
    10081 attcatcttt tcatatactc tggagaaaaa aaaaaaagta attgtagtaa gaatgacaag
    10141 tatcagttga gaaaaaaagt aacaaagaga aagctcggag gaggctcccc aaaggtttta
    10201 tcaccaatgg tggtaccact aacccaagaa cagaaaaagc attgatattt tgaaaaatac
    10261 aatactcatc ttttaaccca aaccttttct tgtcctttaa aacttttatc tcatgtctgt
    10321 ccaaagtcat attgtcttac tactttctct ccttcaacgt ccttccggct ttccccgtca
    10381 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    10441 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    10501 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    10561 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    10621 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
    10681 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    10741 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    10801 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    10861 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    10921 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    10981 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    11041 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    11101 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    11161 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    11221 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    11281 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    11341 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    11401 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    11461 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    11521 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    11581 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    11641 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    11701 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    11761 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    11821 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    11881 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    11941 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    12001 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    12061 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    12121 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    12181 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    12241 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    12301 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    12361 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    12421 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    12481 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    12541 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    12601 gagtcagtga gcgaggaagc gtaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    12661 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    12721 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggc
//
