LOCUS       sequence-24127-e        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme D47A WT*
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    724..740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(748..754,755..770,771..776))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    819..835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(843..849,850..865,866..871))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    931..947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tangaaaaaa gcccgctcat taggcgggct cagatctgct catgttttga cagcttatca
       61 tcgataagct tcggcaataa attcttgata tgttttcata tgtttccttt aaatatttta
      121 attagtaatt gtctattcaa gtaattgtga atatactatc acaattccaa gagaaagtaa
      181 acagctttat agatttttat acgcgtccca agtgccagtt ctaaacgttg taatgactcg
      241 ttttgcgcga ttaggtgttt gattatacca tctactttta gctaagttaa ctgctgcttc
      301 atcccagcgt ttttgttgaa gcatacgtaa agagttagta aatcctgcca caccggtttc
      361 tcccatttgg aaaaccatat taatcaatgc agcgcgacga accgcatcaa gagaatcata
      421 aaccggtttt aatttagcat ttctcagaat tccgcgaaca gcagcatcaa catcctgatt
      481 aaagagtttt tcagcctcat cttttgtaat tacaccatta gtattacgcc caatagcttt
      541 agctaattca gatttagcag cattaagtga tggacttttt gtaagcaaat gaccgatgcc
      601 aatagtgtaa tagccttctg tgtctttata gattttaagt ctaagacctt catctatacg
      661 taacatttca aatatattca taatacctcc taagcatcga tggatcctgt ttcctgtgtg
      721 aaattgttat ccgctcacaa ttccacacat tatacgagcc gatgattaat tgtcaacagg
      781 gggatgggga gtaagctgat cctgtttcct gtgtgaaatt gttatccgct cacaattcca
      841 cacattatac gagccgatga ttaattgtca acagggggga tggggagtaa gctcatcgat
      901 gggatcgatc ctgntttccn ggngggaaaa ttgttatccg ctcacaattc cccacaatta
      961 taccgagncc ggaagcnaaa aagtgnaaaa gccngggggg gccctaangg ngngggccta
     1021 aacttacntt naattggggg tggngctcac tggcccgctt tccggttcgg gaaaacnggt
     1081 ccgggccagg accccatcga aagggggcna aaccntttcn gggnaggggc atg
//
