LOCUS       sequence-241286-        3290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla 
            reniformis with an intron for transient and stable expression in 
            plants .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB123 - pL0_rLUC-I (CDS1)
FEATURES             Location/Qualifiers
     source          1..3290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(27..45)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        113..217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             439..1230
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1401..1989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1890..1909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2143..2160
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaagtggctc ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
       61 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      121 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      181 ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag
      241 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      301 ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
      361 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
      421 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
      481 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
      541 ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg
      601 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
      661 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
      721 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
      781 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
      841 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
      901 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
      961 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1021 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1081 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     1141 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa
     1201 ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt
     1261 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1321 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1381 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1441 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1501 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1561 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1621 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1681 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1741 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1801 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1861 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1921 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1981 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2041 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2101 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2161 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2221 tcactctgtg gtctcaaatg acttctaagg tttatgatcc tgaacaaaga aagagaatga
     2281 ttactggacc tcaatggtgg gctagatgta agcaaatgaa tgttcttgat tcttttatta
     2341 attattatga ttctgaaaag catgctgaaa atgctgttat ttttcttcat ggaaatgctg
     2401 cttcttctta tctttggaga catgttgttc cacatattga accagttgct agatgtatta
     2461 ttccagatct tattggtatg ggaaagtctg gtaagtctgg taatggttct tatagacttc
     2521 ttgatcatta taagtatctt actgcttggt ttgaacttct taatcttcca aagaagatta
     2581 tttttgttgg acatgattgg ggtgcttgtt tggcttttca ttattcttat gaacatcaag
     2641 ataagattaa ggctattgtt catgctgaat ctgttgttga tgttattgaa tcttgggatg
     2701 aatggcctga tattgaagaa gatattgctt tgattaagtc tgaagaagga gaaaagatgg
     2761 ttttggaaaa taatttcttt gttgaaacta tgttgccatc taagattatg agaaagcttg
     2821 aaccagaaga atttgctgct tatcttgaac catttaagga aaagggtgaa gttagaaggc
     2881 caactctttc ttggcctaga gaaattcctc ttgttaaggg tggtaagcct gatgttgttc
     2941 aaattgttag aaattataat gcttatctta gagcttctga tgatcttcca aagatgttta
     3001 ttgaatctga tccaggattc ttttctaatg ctattgttga aggtgctaag aagtttccta
     3061 atactgaatt tgttaaggta cttcatgctt caacgtgtaa cttaagagat actgtgtgaa
     3121 attttatatt tccatacatt tgcttgacct ttgctttttg tcaatttttt tccccttaca
     3181 ggttaaggga cttcattttt ctcaagaaga tgctcctgat gaaatgggaa agtatattaa
     3241 gtcttttgtt gaaagagttc ttaagaatga acaatgagct ttgagaccac
//
