LOCUS       sequence-24130-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme K48-[AAA] WT*
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    730..746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(754..760,761..776,777..782))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    825..841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(849..855,856..871,872..877))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctngaaaagc ccgctcatta ggcgggctca gatctgctca tgtttgacag cttatcatcg
       61 ataagcttcg gcaataaatt cttgatatgt tttcatatgt ttcctttaaa tattttaatt
      121 agtaattgtc tattcaagta attgtgaata tactatcaca attccaagag aaagtaaaca
      181 gctttataga tttttatacg cgtcccaagt gccagttcta aacgttgtaa tgactcgttt
      241 tgcgcgatta ggtgtttgat tataccatct acttttagct aagttaactg ctgcttcatc
      301 ccagcgtttt tgttgaagca tacgtaaaga gttagtaaat cctgccacac cggtttctcc
      361 catttggaaa accatattaa tcaatgcagc gcgacgaacc gcatcaagag aatcataaac
      421 cggttttaat ttagcatttc tcagaattcc gcgaacagca gcatcaacat cctgattaaa
      481 gagtttttca gcctcatctt ttgtaattac accattagta ttacgcccaa tagctgcagc
      541 tgctttatct aattcagatt tagcagcatt aagtgatgga ctttttgtaa gcaaatgacc
      601 gatgccaata gtgtaatagc cttctgtgtc tttatagatt ttaagtctaa gaccttcatc
      661 tatacgtaac atttcaaata tattcataat acctcctaag catcgatgga tcctgtttcc
      721 tgtgtgaaat tgttatccgc tcacaattcc acacattata cgagccgatg attaattgtc
      781 aacaggggga tggggagtaa gctgatcctg tttcctgtgt gaaattgtta tccgctcaca
      841 attccacaca ttatacgagc cgatgattaa ttgtcaacag ggggatgggg agtaagctca
      901 tcgatggatc gatccgggtt ncccgggggg naaatggtan ccgcncccaa ttccccncnt
      961 tatacgagcc ggaagcnnaa agngnaaagc ccgggggggn ccnaaggngg gagcnaactt
     1021 acnttaattg cgntgcgcnc ncgnccnctt tcngncggga aaccngncgg gccngga
//
