LOCUS       sequence-24131-e        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme K48-[AAAA] WT*
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    733..749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(757..763,764..779,780..785))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    828..844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(852..858,859..874,875..880))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctangaaaag ccgctcatta ggcgggctca gatctgctca tgtttgacag cttatcatcg
       61 ataagcttcg gcaataaatt cttgatatgt tttcatatgt ttcctttaaa tattttaatt
      121 agtaattgtc tattcaagta attgtgaata tactatcaca attccaagag aaagtaaaca
      181 gctttataga tttttatacg cgtcccaagt gccagttcta aacgttgtaa tgactcgttt
      241 tgcgcgatta ggtgtttgat tataccatct acttttagct aagttaactg ctgcttcatc
      301 ccagcgtttt tgttgaagca tacgtaaaga gttagtaaat cctgccacac cggtttctcc
      361 catttggaaa accatattaa tcaatgcagc gcgacgaacc gcatcaagag aatcataaac
      421 cggttttaat ttagcatttc tcagaattcc gcgaacagca gcatcaacat cctgattaaa
      481 gagtttttca gcctcatctt ttgtaattac accattagta ttacgcccaa tagctgcagc
      541 tgctgcttta tctaattcag atttagcagc attaagtgat ggactttttg taagcaaatg
      601 accgatgcca atagtgtaat agccttctgt gtctttatag attttaagtc taagaccttc
      661 atctatacgt aacatttcaa atatattcat aatacctcct aagcatcgat ggatcctgtt
      721 tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg atgattaatt
      781 gtcaacaggg ggatggggag taagctgatc ctgtttcctg tgtgaaattg ttatccgctc
      841 acaattccac acattatacg agccgatgat taattgtcaa cagggggatg gggagtaagc
      901 tcatcgatgg atcgaccggg gttnccnggg gggaaaatgg ntaanccgcn cncaantncc
      961 ccnnnttnta cgagccggga agcnnnaagg ggnaagcccg ggggggccca anggngggng
     1021 cnaacntncn tttaantggn gntngnnncn cncngnccnc tttccgnncg ggaaaccngn
     1081 cggggccngg anncccnnnc naangggg
//
