LOCUS       sequence-24133-e        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme K48-[LP] WT*
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    729..745
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(753..759,760..775,776..781))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    824..840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(848..854,855..870,871..876))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gctangaaaa gcccgctcat taggcgggct cagatctgct catgtttgac agcttatcat
       61 cgataagctt cggcaataaa ttcttgatat gttttcatat gtttccttta aatattttaa
      121 ttagtaattg tctattcaag taattgtgaa tatactatca caattccaag agaaagtaaa
      181 cagctttata gatttttata cgcgtcccaa gtgccagttc taaacgttgt aatgactcgt
      241 tttgcgcgat taggtgtttg attataccat ctacttttag ctaagttaac tgctgcttca
      301 tcccagcgtt tttgttgaag catacgtaaa gagttagtaa atcctgccac accggtttct
      361 cccatttgga aaaccatatt aatcaatgca gcgcgacgaa ccgcatcaag agaatcataa
      421 accggtttta atttagcatt tctcagaatt ccgcgaacag cagcatcaac atcctgatta
      481 aagagttttt cagcctcatc ttttgtaatt acaccattag tattacgccc aatagcaggc
      541 agtttatcta attcagattt agcagcatta agtgatggac tttttgtaag caaatgaccg
      601 atgccaatag tgtaatagcc ttctgtgtct ttatagattt taagtctaag accttcatct
      661 atacgtaaca tttcaaatat attcataata cctcctaagc atcgatggat cctgtttcct
      721 gtgtgaaatt gttatccgct cacaattcca cacattatac gagccgatga ttaattgtca
      781 acagggggat ggggagtaag ctgatcctgt ttcctgtgtg aaattgttat ccgctcacaa
      841 ttccacacat tatacgagcc gatgattaat tgtcaacagg gggatgggga gtaagctcat
      901 cgatggatcg anccgggttt cccggnggga aaatngnnan ccgcncncaa ttccccncat
      961 tanacgagcc ggaagcanaa ngggnaagcc nggggggccc nanggggggn gcnacntann
     1021 ttaantgngn tgngcncncn gcccncttnc cnncggaaac cggnnggccg gganccnncn
     1081 aaggggcaaa ccnttcnggn nggnnngann ccccgnaa
//
