LOCUS       sequence-24138-e        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N53A C54A N55A G56A V57A I58A
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    722..738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(746..752,753..768,769..774))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    818..834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(842..848,849..864,865..870))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctnngaaaag cccgctcatt aggcgggctc agatctgctc atgtttgaca gcttatcatc
       61 gataagcttc ggcaataaat tcttgatatg ttttcatatg tttcctttaa atattttaat
      121 tagtaattgt ctattcaagt aattgtgaat atactatcac aattccaaga gaaagtaaac
      181 agctttatag atttttatac gcgtcccaag tgccagttct aaacgttgta atgactcgtt
      241 ttgcgcgatt aggtgtttga ttataccatc tacttttagc taagttaact gctgcttcat
      301 cccagcgttt ttgttgaagc atacgtaaag agttagtaaa tcctgccaca ccggtttctc
      361 ccatttggaa aaccatatta atcaatgcac agcgacgaac cgcatcaaga gaatcataaa
      421 ccggttttaa tttagcattt ctcagaattc cgcgaacagc agcatcaaca tcctgattaa
      481 agagtttttc agcctcatct tttgtagctg cagcagcggc agcacgccca atagctttat
      541 ctaattcaga tttagcagca ttaagtgatg gactttttgt aagcaaatga ccgatgccaa
      601 tagtgtaata gccttctgtg tctttataga ttttaagtct aagaccttca tctatacgta
      661 acatttcaaa tatattcata atacctccta agcatcgatg gatcctgttt cctgtgtgaa
      721 attgttatcc gctcacaatt ccacacatta tacgagccga tgattaattg tcaacagggg
      781 gatgggggag taagctgatc ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      841 acattatacg agccgatgat taattgtcaa cagggggatg gggagtaagc tcatcgatgg
      901 atcgatcngg tttcctgggg gaaaatggtn atccgctcac aattccccnc attatacgag
      961 ccggaancat aaaggggnaa agcctggggg ggcctaangn ggngngctaa cttacattaa
     1021 ttggngntgg ggcnncacgg ccccctttcc anncggggaa accnggtcgg ggcnnggaca
     1081 cccatcnaaa gggggcaaaa cctttcgggg gnang
//
