LOCUS       sequence-24139-e        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N53A C54A N55A G56A V57A I58A E62A
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    720..736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(744..750,751..766,767..772))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    815..831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(839..845,846..861,862..867))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 angaaaagcc cgctcattag gcgggctcag atctgctcat gtttgacagc ttatcatcga
       61 taagcttcgg caataaattc ttgatatgtt ttcatatgtt tcctttaaat attttaatta
      121 gtaattgtct attcaagtaa ttgtgaatat actatcacaa ttccaagaga aagtaaacag
      181 ctttatagat ttttatacgc gtcccaagtg ccagttctaa acgttgtaat gactcgtttt
      241 gcgcgattag gtgtttgatt ataccatcta cttttagcta agttaactgc tgcttcatcc
      301 cagcgttttt gttgaagcat acgtaaagag ttagtaaatc ctgccacacc ggtttctccc
      361 atttggaaaa ccatattaat caatgcacag cgacgaaccg catcaagaga atcataaacc
      421 ggttttaatt tagcatttct cagaattccg cgaacagcag catcaacatc ctgattaaag
      481 agtttttcag ccgcatcttt tgtagctgca gcagcggcag cacgcccaat agctttatct
      541 aattcagatt tagcagcatt aagtgatgga ctttttgtaa gcaaatgacc gatgccaata
      601 gtgtaatagc cttctgtgtc tttatagatt ttaagtctaa gaccttcatc tatacgtaac
      661 atttcaaata tattcataat acctcctaag catcgatgga tcctgtttcc tgtgtgaaat
      721 tgttatccgc tcacaattcc acacattata cgagccgatg attaattgtc aacaggggga
      781 tggggagtaa gctgatcctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      841 ttatacgagc cgatgattaa ttgtcaacag ggggatgggg agtaagctca tcgatggatc
      901 gaccgggttt cccgggggga aantggttat ccngcncnca attccccnca ttntacnanc
      961 cggaaagcnn aaagggnnaa agccnggggg gnccnaanga gngnncnnaa cttacnttaa
     1021 ntgngttgcg cncncggncc gcnttncnnn cgggaaaccn gncngggccn ggnncccnnc
     1081 naanggg
//
