LOCUS       sequence-24140-e        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N53A N55A V57A
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    721..737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(745..751,752..767,768..773))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    816..832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(840..846,847..862,863..868))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctangaaaag ccgctcatta ggcgggctca gatctgctca tgtttgacag cttatcatcg
       61 atcngcttcg gcaataaatt cttgatatgt tttcatatgt ttcctttaaa tattttaatt
      121 agtaattgtc tattcaagta attgtgaata tactatcaca attccaagag aaagtaaaca
      181 gctttataga tttttatacg cgtcccaagt gccagttcta aacgttgtaa tgactcgttt
      241 tgcgcgatta ggtgtttgat tataccatct acttttagct aagttaactg ctgcttcatc
      301 ccagcgtttt tgttgaagca tacgtaaaga gttagtaaat cctgccacac cggtttctcc
      361 catttggaaa accatattaa tcaatgcaca gcgacgaacc gcatcaagag aatcataaac
      421 cggttttaat ttagcatttc tcagaattcc gcgaacagca gcatcaacat cctgattaaa
      481 gagtttttca gcctcatctt ttgtaattgc accagcgcaa gcacgcccaa tagctttatc
      541 taattcagat ttagcagcat taagtgatgg actttttgta agcaaatgac cgatgccaat
      601 agtgtaatag ccttctgtgt ctttatagat tttaagtcta agaccttcat ctatacgtaa
      661 catttcaaat atattcataa tacctcctaa gcatcgatgg atcctgtttc ctgtgtgaaa
      721 ttgttatccg ctcacaattc cacacattat acgagccgat gattaattgt caacaggggg
      781 atggggagta agctgatcct gtttcctgtg tgaaattgtt atccgctcac aattccncac
      841 attatacgag ccgatgatta attgtcaaca gggggatggg gagtaagctc atcgatggat
      901 cgacccggnt tnccgggggg aaatggttat ccgcncncna ttccncncat tanacgagcc
      961 ggaagcanaa agnggnaang ccnggggggn ccnaaggagg gancnaactt acnttaantg
     1021 gcgttgnngc ncccnggccc gnctttccng nncgggnaaa ccnggncggg gccg
//
