LOCUS       sequence-241413-        3156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata 
            with an intron for transient and stable expression in plants (moved 
            to a CDS1 position from pICSL50016; Engler et al., 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB65 - pL0_tGFP-I (CDS1)
FEATURES             Location/Qualifiers
     source          1..3156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        46..150
                     /gene="bla"
                     /label=AmpR promoter
     CDS             372..1163
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1334..1922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1823..1842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2076..2093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     intron          2552..2740
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             complement(3048..3065)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     primer_bind     complement(3116..3134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
       61 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      121 atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc cagaaccttg
      181 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
      241 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
      301 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
      361 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
      421 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
      481 cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg gtgaccgtaa
      541 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
      601 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
      661 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
      721 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
      781 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
      841 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
      901 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
      961 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     1021 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     1081 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta aaaggcgaga
     1141 tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata ctttagattg
     1201 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1261 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1321 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1381 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1441 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1501 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1561 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1621 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1681 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1741 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1801 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1861 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1921 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1981 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2041 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2101 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct
     2161 gtggtctcaa atgagaggat ctggatctga gtctgatgag tctggacttc ctgctatgga
     2221 aatcgagtgt agaatcactg gaacccttaa cggtgttgag ttcgagcttg ttggaggtgg
     2281 tgagggaact cctgagcagg gaagaatgac taacaagatg aagtctacca agggtgctct
     2341 taccttctct ccataccttc tttctcacgt tatgggatac ggattctacc acttcggaac
     2401 ttacccatct ggatacgaga accctttcct tcatgctatc aacaacggtg gatacaccaa
     2461 cactaggatc gagaagtacg aggatggtgg tgttcttcac gttagcttct cttacagata
     2521 cgaggctgga agagtgatcg gagatttcaa ggtaagtttc tgcttctacc tttgatatat
     2581 atataataat tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa
     2641 aagaatgtag tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta
     2701 taacttttct aatatatgac caaaatttgt tgatgtgcag gttatgggaa ctggattccc
     2761 tgaggattct gttatcttca ccgacaagat catcaggtct aacgctactg ttgagcatct
     2821 tcatcctatg ggagataacg atctcgatgg atctttcacc agaaccttct cacttagaga
     2881 tggtggttac tactcttctg tggtggattc tcacatgcac ttcaagtctg ctatccaccc
     2941 ttctatcctt caaaacggtg gacctatgtt cgctttcaga agagttgagg aagatcactc
     3001 taacaccgag cttggaatcg ttgagtacca acatgctttc aagacccctg atgctgatgc
     3061 tggtgaggaa tgataagctt tgagaccacg aagtggctct tcagtggacg aaagggcctc
     3121 gtgatacgcc tatttttata ggttaatgtc atgata
//
