LOCUS       sequence-241416-        2601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus 
            (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence 
            NC_004005.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB61 - pL0_V2 (CDS1)
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..60)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        128..232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             454..1245
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1416..2004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1905..1924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2158..2175
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagctttgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt
       61 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      121 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      181 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc
      241 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
      301 catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag
      361 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
      421 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta
      481 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
      541 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat
      601 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
      661 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
      721 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
      781 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
      841 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
      901 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
      961 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     1021 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     1081 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     1141 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     1201 gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt
     1261 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1321 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1381 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1441 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1501 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1561 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1621 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1681 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1741 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1801 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1861 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1921 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1981 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2041 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2101 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2161 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2221 tggccgattc attaatcact ctgtggtctc aaatgtggga cccacttcta aatgaatttc
     2281 ctgaatctgt tcacggattt cgttgtatgt tagctattaa atatttgcag tccgttgagg
     2341 aaacttacga gcccaataca ttgggccacg atttaattag ggatcttata tctgttgtaa
     2401 gggcccgtga ctatgtcgaa gcgaccaggc gatataatca tttccacgcc agactcgaag
     2461 gttcgccgaa ggctgaactt cgacagccca tacagcagcc gtgctgctgt ccccattgtc
     2521 caaggcacaa acaagcgacg atcatggacg tacaggccca tgtaccgaaa gcccagaata
     2581 tacagaatgt atcgaagccc t
//
