LOCUS       sequence-241417-        2787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate (MoClo; CDS1) compatible ZCT1 gene from Catharanthus 
            roseus; a repressor of the STR1 promoter from C. roseus .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB139 - pL0_ZCT1 (CDS1)
FEATURES             Location/Qualifiers
     source          1..2787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             369..1160
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1331..1919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1820..1839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2073..2090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2744..2762)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
       61 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      121 cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag aaccttgacc
      181 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
      241 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
      301 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
      361 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
      421 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
      481 tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc
      541 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
      601 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
      661 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
      721 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
      781 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
      841 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
      901 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
      961 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     1021 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     1081 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca
     1141 ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt
     1201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1441 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1981 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2041 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2101 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg
     2161 gtctcaaatg ggcgtgaaga gattcagaga agaacaatcc gcaaattgtt tgatgcttct
     2221 ttctaaagtc ggattattat cagaaatgga gaaatcagca gtaccggcgt tgaaacccgg
     2281 cgccggcgga cgaatttatg agtgcaaaac ttgtaaaaag caatttctca cttttcaggc
     2341 tttaggcggt caccgagcga gtcacaagaa gcttagatta atggcggcag atctactcca
     2401 tcaatcttta gcggtgacga agccgaaaac tcatgcttgt tctatatgtg gcctcgaatt
     2461 tccattaggt caggctctcg gaggtcatat gagacgccac cgcggcgccg ccttggatgg
     2521 agagaaaccg gtggtggtga gtgataaacc tgttgcaaag gcggtaccat tcctgatgag
     2581 atctaatagc agtaagagga tttttggatt tgaagttgac ggattggatc tgaatttgac
     2641 gcctgaggac aacgatccgg acgagcgaga caagtttcct cccttactgg aatttttcgt
     2701 ctaagctttg agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta
     2761 tttttatagg ttaatgtcat gataata
//
