LOCUS       sequence-241418-        2853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus 
            roseus; an activator of the STR1 promoter from C. roseus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB142 - pL0_ORCA3 (CDS1)
FEATURES             Location/Qualifiers
     source          1..2853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             388..1179
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1350..1938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1839..1858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2092..2109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2829..2847)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
       61 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      121 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
      181 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      241 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
      301 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
      361 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
      421 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
      481 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
      541 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
      601 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
      661 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
      721 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
      781 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
      841 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
      901 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
      961 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     1021 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     1081 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat
     1141 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca
     1201 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1261 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1321 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1381 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1441 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1501 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1561 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1621 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1681 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1741 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1801 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1861 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1921 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1981 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2041 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2101 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2161 attcattaat cactctgtgg tctcaaatgt ccgaagaaat catttccgtc tcagatcgat
     2221 ttcttctttc cttaatcgaa gaacatcttc tcagcgataa ttctgatgat tccagctcgg
     2281 aattgacttc tacagaggaa aattgggaag aaatttttgc agattttcta aattggtcgg
     2341 gatccgaaat acagaaacgc ggtagcccga gttccgaaag ctgtcaatcg aattcaatgg
     2401 cggaaagctg tcaggaggat tctgttgtgg gaaccccgcc agaagcggcg gccggaggag
     2461 gttgttcgaa ggattggaac cggtataagg gcgttagacg gcggccgtgg gggaagttcg
     2521 cggcggagat aagggatccg aaaaagaaag gatccaggat ttggttgggt acatacgaga
     2581 cacctgagga tgcagcattg gcttatgatg cagccgcgtt taatatgcgt ggagctaaag
     2641 ctaggcttaa ttttcctcat ttgattggtt cgaatatttc cggacccgtt agagtaaacc
     2701 cgagaaaacg tttccctgcg gagccttcta cgacgtcgtc gtcatcttcg tctgaaaata
     2761 gtggaggaag gaagaagaga cgatattaag ctttgagacc acgaagtggc tcttcagtgg
     2821 acgaaagggc ctcgtgatac gcctattttt ata
//
