LOCUS       sequence-241419-       11833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PDGFRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PDGFRB
FEATURES             Location/Qualifiers
     source          1..11833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4984..5130
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     protein_bind    complement(6770..6794)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6847..6888
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6909..7476
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6926..7478
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7070..7087)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7297..7316
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7490..7492,7493..7495,7496..8209)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7490..7492,7493..7495,7496..8206)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7535..7556)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7796..7815)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8143..8164
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8223..8811
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8276..8296)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8694..8705)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8886..9119
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9238..9256)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9324..9428
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9429..9497,9498..10289)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9647..9666)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10460..11048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10949..10968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11202..11219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11459..11480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11495..11500,11501..11518,11519..11525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11533..11549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11538..11560
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11557..11573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11557..11573
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11623..11642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cggcttccgg gtgcgatgcc agctctggcc
     3481 ctcaaaggcg agctgctgtt gctgtctctc ctgttacttc tggaaccaca gatctctcag
     3541 ggcctggtcg tcacaccccc ggggccagag cttgtcctca atgtctccag caccttcgtt
     3601 ctgacctgct cgggttcagc tccggtggtg tgggaacgga tgtcccagga gcccccacag
     3661 gaaatggcca aggcccagga tggcaccttc tccagcgtgc tcacactgac caacctcact
     3721 gggctagaca cgggagaata cttttgcacc cacaatgact cccgtggact ggagaccgat
     3781 gagcggaaac ggctctacat ctttgtgcca gatcccaccg tgggcttcct ccctaatgat
     3841 gccgaggaac tattcatctt tctcacggaa ataactgaga tcaccattcc atgccgagta
     3901 acagacccac agctggtggt gacactgcac gagaagaaag gggacgttgc actgcctgtc
     3961 ccctatgatc accaacgtgg cttttttggt atctttgagg acagaagcta catctgcaaa
     4021 accaccattg gggacaggga ggtggattct gatgcctact atgtctacag actccaggtg
     4081 tcatccatca acgtctctgt gaacgcagtg cagactgtgg tccgccaggg tgagaacatc
     4141 accctcatgt gcattgtgat cgggaatgag gtggtcaact tcgagtggac atacccccgc
     4201 aaagaaagtg ggcggctggt ggagccggtg actgacttcc tcttggatat gccttaccac
     4261 atccgctcca tcctgcacat ccccagtgcc gagttagaag actcggggac ctacacctgc
     4321 aatgtgacgg agagtgtgaa tgaccatcag gatgaaaagg ccatcaacat caccgtggtt
     4381 gagagcggct acgtgcggct cctgggagag gtgggcacac tacaatttgc tgagctgcat
     4441 cggagccgga cactgcaggt agtgttcgag gcctacccac cgcccactgt cctgtggttc
     4501 aaagacaacc gcaccctggg cgactccagc gctggcgaaa tcgccctgtc cacgcgcaac
     4561 gtgtcggaga cccggtatgt gtcagagctg acactggttc gcgtgaaggt ggcagaggct
     4621 ggccactaca ccatgcgggc cttccatgag gatgctgagg tccagctctc cttccagcta
     4681 cagatcaatg tccctgtccg agtgctggag ctaagtgaga gccaccctga cagtggggaa
     4741 cagacagtcc gctgtcgtgg ccggggcatg ccccagccga acatcatctg gtctgcctgc
     4801 agagacctca aaaggtgtcc acgtgagctg ccgcccacgc tgctggggaa cagttccgaa
     4861 gaggagagcc agctggagac taacgtgacg tactgggagg aggagcagga gtttgaggtg
     4921 gtgagcacac tgcgtctgca gcacgtggat cggccactgt cggtgcgctg cacgctgcgc
     4981 aacgctgtgg gccaggacac gcaggaggtc atcgtggtgc cacactcctt gccctttaag
     5041 gtggtggtga tctcagccat cctggccctg gtggtgctca ccatcatctc ccttatcatc
     5101 ctcatcatgc tttggcagaa gaagccacgt tacgagatcc gatggaaggt gattgagtct
     5161 gtgagctctg acggccatga gtacatctac gtggacccca tgcagctgcc ctatgactcc
     5221 acgtgggagc tgccgcggga ccagcttgtg ctgggacgca ccctcggctc tggggccttt
     5281 gggcaggtgg tggaggccac ggctcatggc ctgagccatt ctcaggccac gatgaaagtg
     5341 gccgtcaaga tgcttaaatc cacagcccgc agcagtgaga agcaagccct tatgtcggag
     5401 ctgaagatca tgagtcacct tgggccccac ctgaacgtgg tcaacctgtt gggggcctgc
     5461 accaaaggag gacccatcta tatcatcact gagtactgcc gctacggaga cctggtggac
     5521 tacctgcacc gcaacaaaca caccttcctg cagcaccact ccgacaagcg ccgcccgccc
     5581 agcgcggagc tctacagcaa tgctctgccc gttgggctcc ccctgcccag ccatgtgtcc
     5641 ttgaccgggg agagcgacgg tggctacatg gacatgagca aggacgagtc ggtggactat
     5701 gtgcccatgc tggacatgaa aggagacgtc aaatatgcag acatcgagtc ctccaactac
     5761 atggcccctt acgataacta cgttccctct gcccctgaga ggacctgccg agcaactttg
     5821 atcaacgagt ctccagtgct aagctacatg gacctcgtgg gcttcagcta ccaggtggcc
     5881 aatggcatgg agtttctggc ctccaagaac tgcgtccaca gagacctggc ggctaggaac
     5941 gtgctcatct gtgaaggcaa gctggtcaag atctgtgact ttggcctggc tcgagacatc
     6001 atgcgggact cgaattacat ctccaaaggc agcacctttt tgcctttaaa gtggatggct
     6061 ccggagagca tcttcaacag cctctacacc accctgagcg acgtgtggtc cttcgggatc
     6121 ctgctctggg agatcttcac cttgggtggc accccttacc cagagctgcc catgaacgag
     6181 cagttctaca atgccatcaa acggggttac cgcatggccc agcctgccca tgcctccgac
     6241 gagatctatg agatcatgca gaagtgctgg gaagagaagt ttgagattcg gccccccttc
     6301 tcccagctgg tgctgcttct cgagagactg ttgggcgaag gttacaaaaa gaagtaccag
     6361 caggtggatg aggagtttct gaggagtgac cacccagcca tccttcggtc ccaggcccgc
     6421 ttgcctgggt tccatggcct ccgatctccc ctggacacca gctccgtcct ctatactgcc
     6481 gtgcagccca atgagggtga caacgactat atcatccccc tgcctgaccc caaacccgag
     6541 gttgctgacg agggcccact ggagggttcc cccagcctag ccagctccac cctgaatgaa
     6601 gtcaacacct cctcaaccat ctcctgtgac agccccctgg agccccagga cgaaccagag
     6661 ccagagcccc agcttgagct ccaggtggag ccggagccag agctggaaca gttgccggat
     6721 tcggggtgcc ctgcgcctcg ggcggaagca gaggatagct tcctgtagaa cccagctttc
     6781 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
     6841 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag
     6901 taatgagatc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6961 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     7021 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     7081 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     7141 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     7201 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     7261 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     7321 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     7381 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     7441 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacacata tggtgagcaa
     7501 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     7561 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     7621 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     7681 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     7741 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     7801 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     7861 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     7921 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     7981 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     8041 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     8101 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     8161 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa tcgatagatc
     8221 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     8281 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     8341 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     8401 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     8461 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     8521 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     8581 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     8641 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     8701 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8761 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     8821 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8881 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8941 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9001 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9061 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9121 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     9181 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     9241 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     9301 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9361 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9421 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9481 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9541 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9601 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9661 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9721 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9781 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9841 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9901 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9961 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10021 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10081 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10141 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10201 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10261 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10321 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10381 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10441 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10501 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10561 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10621 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10681 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10741 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10801 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10861 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10921 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10981 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11041 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11101 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11161 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11221 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11281 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    11341 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    11401 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11461 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11521 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    11581 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11641 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    11701 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    11761 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11821 caaacttagt agt
//
