LOCUS       sequence-24142-e        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme C54S C97A
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    726..742
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(750..756,757..772,773..778))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(829..845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(829..845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    853..869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctangaaaaa gcccgctcat taggcgggct cagatctgct catggtttga cagcttatca
       61 tcgcgataag cttcggcaat aaattcttga tatgttttca tatgtttcct ttaaatattt
      121 taattagtaa ttgtctattc aagtaattgt gaatatacta tcacaattcc aagagaaagt
      181 aaacagcttt atagattttt atacgcgtcc caagtgccag ttctaaacgt tgtaatgact
      241 cgttttgcgc gattaggtgt ttgattatac catctacttt tagctaagtt aactgctgct
      301 tcatcccagc gtttttgttg aagcatacgt aaagagttag taaatcctgc cacaccggtt
      361 tctcccattt ggaaaaccat attaatcaat gcagcgcgac gaaccgcatc aagagaatca
      421 taaaccggtt ttaatttagc atttctcaga attccgcgaa cagcagcatc aacatcctga
      481 ttaaagagtt tttcagcctc atcttttgta attacaccat tactattacg cccaatagct
      541 ttatctaatt cagatttagc agcattaagt gatggacttt ttgtaagcaa atgaccgatg
      601 ccaatagtgt aatagccttc tgtgtcttta tagattttaa gtctaagacc ttcatctata
      661 cgtaacattt caaatatatt cataatacct cctaagcatc gatggatcct gtttcctgtg
      721 tgaaattgtt atccgctcac aattccacac attatacgag ccgatgatta attgtcaaca
      781 gggggatggg gagtaagctc atcgcgatgt gnaatccgnt aantcatggt catagctgtt
      841 tcctgtgtga aattgttatc cgctcacaat tccncacatt atacgagccg gnagcataaa
      901 gngtaagcct ggggggncct aatgagtgag ctacttacat naatngcgtt gcgctcactg
      961 nccgctttcc agtcgggaaa cccngtcgng cnaggacccc atcgaanggg gcaaaacctt
     1021 tcccgggntg gcatgnaagc ccccnggaan anagtcaaat cngggggggg nangggaaan
     1081 ccngtaacgt tntacna
//
