LOCUS       sequence-241420-        2839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate (MoClo; PRO + 5U) compatible STR1 promoter from 
            Catharanthus roseus .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB62 - pL0_STR (pro + 5U)
FEATURES             Location/Qualifiers
     source          1..2839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        67..171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             393..1184
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1355..1943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1844..1863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2097..2114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2820..2838)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
       61 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca
      181 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
      241 atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc
      301 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
      361 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
      421 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
      481 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt
      541 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
      601 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
      661 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
      721 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
      781 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
      841 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
      901 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
      961 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     1021 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     1081 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     1141 tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt
     1201 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1261 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1321 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1381 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1441 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1501 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1561 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1621 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1681 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1741 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1801 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1861 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1921 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1981 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2041 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2101 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2161 ggccgattca ttaatcactc tgtggtctca ggaggatctt tattgtttgt attttcataa
     2221 ttaattaatc cctacaactg atttcaaaaa aaaatctatt tgaaaaaaat aattctgcta
     2281 ctaaaaattt taaaatcttc ctataatatc attttacgag tttaatttat ttattatcat
     2341 attaaaacaa taatattatt cattacagaa attggacaaa caaaaagatc tagcaaaagt
     2401 attatgattt taatatatag tcgaaaatac gaattagaca agcgaaacat tttgtaatta
     2461 aatcgctatt tatcatataa ttattttaca ataattagta ttaggaatta taattattaa
     2521 ttgatgtata aaccgataaa aatatattta tgatatatac aaaataaagg aataaaagaa
     2581 tgcataagta tattggataa gcctataaat attactccac gtggtacatc actcttagac
     2641 cgccttcttt gaaagtgatt tcccttggac cttgtttggt gagtttgtgg gcttattata
     2701 ttccttcact gtccctctaa gtatcaaacc ataccccata gctgcccctc tctgcttcac
     2761 tcccatcatt tacagtcaac aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg
     2821 cctcgtgata cgcctattt
//
