LOCUS       sequence-241439-       11711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GUCY2C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-GUCY2C
FEATURES             Location/Qualifiers
     source          1..11711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6671..6695)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6703..6721
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6746..6765)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6747..6765)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6771..6791)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6845..6886
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6907..7474
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6924..7476
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7068..7085)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7295..7314
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7488..8087
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7488..7507)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7984..8004
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8101..8689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8154..8174)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8572..8583)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8764..8997
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9116..9134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9202..9306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9307..9375,9376..10167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9525..9544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10338..10926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10827..10846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11080..11097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11337..11358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11373..11378,11379..11396,11397..11403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11411..11427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11416..11438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11435..11451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11435..11451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11501..11520
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg aagacgttgc tgttggactt ggctttgtgg
     3481 tcactgctct tccagcccgg gtggctgtcc tttagttccc aggtgagtca gaactgccac
     3541 aatggcagct atgaaatcag cgtcctgatg atgggcaact cagcctttgc agagcccctg
     3601 aaaaacttgg aagatgcggt gaatgagggg ctggaaatag tgagaggacg tctgcaaaat
     3661 gctggcctaa atgtgactgt gaacgctact ttcatgtatt cggatggtct gattcataac
     3721 tcaggcgact gccggagtag cacctgtgaa ggcctcgacc tactcaggaa aatttcaaat
     3781 gcacaacgga tgggctgtgt cctcataggg ccctcatgta catactccac cttccagatg
     3841 taccttgaca cagaattgag ctaccccatg atctcagctg gaagttttgg attgtcatgt
     3901 gactataaag aaaccttaac caggctgatg tctccagcta gaaagttgat gtacttcttg
     3961 gttaactttt ggaaaaccaa cgatctgccc ttcaaaactt attcctggag cacttcgtat
     4021 gtttacaaga atggtacaga aactgaggac tgtttctggt accttaatgc tctggaggct
     4081 agcgtttcct atttctccca cgaactcggc tttaaggtgg tgttaagaca agataaggag
     4141 tttcaggata tcttaatgga ccacaacagg aaaagcaatg tgattattat gtgtggtggt
     4201 ccagagttcc tctacaagct gaagggtgac cgagcagtgg ctgaagacat tgtcattatt
     4261 ctagtggatc ttttcaatga ccagtacttg gaggacaatg tcacagcccc tgactatatg
     4321 aaaaatgtcc ttgttctgac gctgtctcct gggaattccc ttctaaatag ctctttctcc
     4381 aggaatctat caccaacaaa acgagacttt gctcttgcct atttgaatgg aatcctgctc
     4441 tttggacata tgctgaagat atttcttgaa aatggagaaa atattaccac ccccaaattt
     4501 gctcatgctt tcaggaatct cacttttgaa gggtatgacg gtccagtgac cttggatgac
     4561 tggggggatg ttgacagtac catggtgctt ctgtatacct ctgtggacac caagaaatac
     4621 aaggttcttt tgacctatga tacccacgta aataagacct atcctgtgga tatgagcccc
     4681 acattcactt ggaagaactc taaacttcct aatgatatta caggccgggg ccctcagatc
     4741 ctgatgattg cagtcttcac cctcactgga gctgtggtgc tgctcctgct cgtcgctctc
     4801 ctgatgctca gaaaatatag aaaagattat gaacttcgtc agaaaaaatg gtcccacatt
     4861 cctcctgaaa atatctttcc tctggagacc aatgagacca atcatgttag cctcaagatc
     4921 gatgatgaca aaagacgaga tacaatccag agactacgac agtgcaaata cgacaaaaag
     4981 cgagtgattc tcaaagatct caagcacaat gatggtaatt tcactgaaaa acagaagata
     5041 gaattgaaca agttgcttca gattgactat tacaacctga ccaagttcta cggcacagtg
     5101 aaacttgata ccatgatctt cggggtgata gaatactgtg agagaggatc cctccgggaa
     5161 gttttaaatg acacaatttc ctaccctgat ggcacattca tggattggga gtttaagatc
     5221 tctgtcttgt atgacattgc taagggaatg tcatatctgc actccagtaa gacagaagtc
     5281 catggtcgtc tgaaatctac caactgcgta gtggacagta gaatggtggt gaagatcact
     5341 gattttggct gcaattccat tttacctcca aaaaaggacc tgtggacagc tccagagcac
     5401 ctccgccaag ccaacatctc tcagaaagga gatgtgtaca gctatgggat catcgcacag
     5461 gagatcattc tgcggaaaga aaccttctac actttgagct gtcgggaccg gaatgagaag
     5521 attttcagag tggaaaattc caatggaatg aaacccttcc gcccagattt attcttggaa
     5581 acagcagagg aaaaagagct agaagtgtac ctacttgtaa aaaactgttg ggaggaagat
     5641 ccagaaaaga gaccagattt caaaaaaatt gagactacac ttgccaagat atttggactt
     5701 tttcatgacc aaaaaaatga aagctatatg gataccttga tccgacgtct acagctatat
     5761 tctcgaaacc tggaacatct ggtagaggaa aggacacagc tgtacaaggc agagagggac
     5821 agggctgaca gacttaactt tatgttgctt ccaaggctag tggtaaagtc tctgaaggag
     5881 aaaggctttg tggagccgga actatatgag gaagttacaa tctacttcag tgacattgta
     5941 ggtttcacta ctatctgcaa atacagcacc cccatggaag tggtggacat gcttaatgac
     6001 atctataaga gttttgacca cattgttgat catcatgatg tctacaaggt ggaaaccatc
     6061 ggtgatgcgt acatggtggc tagtggtttg cctaagagaa atggcaatcg gcatgcaata
     6121 gacattgcca agatggcctt ggaaatcctc agcttcatgg ggacctttga gctggagcat
     6181 cttcctggcc tcccaatatg gattcgcatt ggagttcact ctggtccctg tgctgctgga
     6241 gttgtgggaa tcaagatgcc tcgttattgt ctatttggag atacggtcaa cacagcctct
     6301 aggatggaat ccactggcct ccctttgaga attcacgtga gtggctccac catagccatc
     6361 ctgaagagaa ctgagtgcca gttcctttat gaagtgagag gagaaacata cttaaaggga
     6421 agaggaaatg agactaccta ctggctgact gggatgaagg accagaaatt caacctgcca
     6481 acccctccta ctgtggagaa tcaacagcgt ttgcaagcag aattttcaga catgattgcc
     6541 aactctttac agaaaagaca ggcagcaggg ataagaagcc aaaaacccag acgggtagcc
     6601 agctataaaa aaggcactct ggaatacttg cagctgaata ccacagacaa ggagagcacc
     6661 tatttttaga acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag
     6721 gtttgtgtgt tattgtaatt gagatcccta tagtgagtcg tattaccacc caacttttct
     6781 atacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     6841 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta
     6901 atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     6961 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     7021 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     7081 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     7141 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     7201 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     7261 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     7321 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     7381 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     7441 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca
     7501 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     7561 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     7621 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     7681 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     7741 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     7801 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     7861 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     7921 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7981 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     8041 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct
     8101 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8161 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8221 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8281 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8341 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8401 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8461 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8521 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8581 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8641 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt
     8701 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8761 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     8821 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8881 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8941 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     9001 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9061 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc
     9121 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     9181 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     9241 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     9301 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9361 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9421 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9481 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9541 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9601 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9661 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9721 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9781 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9841 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9901 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     9961 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10021 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10081 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10141 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10201 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10261 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10321 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10381 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10441 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10501 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10561 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10621 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10681 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10741 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10801 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10861 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10921 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    10981 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    11041 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    11101 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    11161 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    11221 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    11281 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    11341 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    11401 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
    11461 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    11521 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    11581 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    11641 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    11701 aacttagtag t
//
