LOCUS       sequence-241441-       11548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MAN2B1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-MAN2B1
FEATURES             Location/Qualifiers
     source          1..11548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6485..6509)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6562..6603
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6624..7191
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6641..7193
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6785..6802)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7012..7031
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7205..7207,7208..7210,7211..7924)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7205..7207,7208..7210,7211..7921)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7250..7271)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7511..7530)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7858..7879
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7938..8526
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7991..8011)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8409..8420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8601..8834
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8953..8971)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9039..9143
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9144..9212,9213..10004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9362..9381)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10175..10763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10664..10683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10917..10934
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11174..11195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11210..11215,11216..11233,11234..11240)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11248..11264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11253..11275
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11272..11288
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11272..11288
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11338..11357
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg ggcgcctacg cgcgggcttc gggggtctgc
     3481 gctcgcggct gcctggactc agcaggcccc tggaccatgt cccgcgccct gcggccaccg
     3541 ctcccgcctc tctgcttttt ccttttgttg ctggcggctg ccggtgctcg ggccggggga
     3601 tacgagacat gccccacagt gcagccgaac atgctgaacg tgcacctgct gcctcacaca
     3661 catgatgacg tgggctggct caaaaccgtg gaccagtact tttatggaat caagaatgac
     3721 atccagcacg ccggtgtgca gtacatcctg gactcggtca tctctgcctt gctggcagat
     3781 cccacccgtc gcttcattta cgtggagatt gccttcttct cccgttggtg gcaccagcag
     3841 acaaatgcca cacaggaagt cgtgcgagac cttgtgcgcc aggggcgcct ggagttcgcc
     3901 aatggtggct gggtgatgaa cgatgaggca gccacccact acggtgccat cgtggaccag
     3961 atgacacttg ggctgcgctt tctggaggac acatttggca atgatgggcg accccgtgtg
     4021 gcctggcaca ttgacccctt cggccactct cgggagcagg cctcgctgtt tgcgcagatg
     4081 ggcttcgacg gcttcttctt tgggcgcctt gattatcaag ataagtgggt acggatgcag
     4141 aagctggaga tggagcaggt gtggcgggcc agcaccagcc tgaagccccc gaccgcggac
     4201 ctcttcactg gtgtgcttcc caatggttac aacccgccaa ggaatctgtg ctgggatgtg
     4261 ctgtgtgtcg atcagccgct ggtggaggac cctcgcagcc ccgagtacaa cgccaaggag
     4321 ctggtcgatt acttcctaaa tgtggccact gcccagggcc ggtattaccg caccaaccac
     4381 actgtgatga ccatgggctc ggacttccaa tatgagaatg ccaacatgtg gttcaagaac
     4441 cttgacaagc tcatccggct ggtaaatgcg cagcaggcaa aaggaagcag tgtccatgtt
     4501 ctctactcca cccccgcttg ttacctctgg gagctgaaca aggccaacct cacctggtca
     4561 gtgaaacatg acgacttctt cccttacgcg gatggccccc accagttctg gaccggttac
     4621 ttttccagtc ggccggccct caaacgctac gagcgcctca gctacaactt cctgcaggtg
     4681 tgcaaccagc tggaggcgct ggtgggcctg gcggccaacg tgggacccta tggctccgga
     4741 gacagtgcac ccctcaatga ggcgatggct gtgctccagc atcacgacgc cgtcagcggc
     4801 acctcccgcc agcacgtggc caacgactac gcgcgccagc ttgcggcagg ctgggggcct
     4861 tgcgaggttc ttctgagcaa cgcgctggcg cggctcagag gcttcaaaga tcacttcacc
     4921 ttttgccaac agctaaacat cagcatctgc ccgctcagcc agacggcggc gcgcttccag
     4981 gtcatcgttt ataatcccct ggggcggaag gtgaattgga tggtacggct gccggtcagc
     5041 gaaggcgttt tcgttgtgaa ggaccccaat ggcaggacag tgcccagcga tgtggtaata
     5101 tttcccagct cagacagcca ggcgcaccct ccggagctgc tgttctcagc ctcactgccc
     5161 gccctgggct tcagcaccta ttcagtagcc caggtgcctc gctggaagcc ccaggcccgc
     5221 gcaccacagc ccatccccag aagatcctgg tcccctgctt taaccatcga aaatgagcac
     5281 atccgggcaa cgtttgatcc tgacacaggg ctgttgatgg agattatgaa catgaatcag
     5341 caactcctgc tgcctgttcg ccagaccttc ttctggtaca acgccagtat aggtgacaac
     5401 gaaagtgacc aggcctcagg tgcctacatc ttcagaccca accaacagaa accgctgcct
     5461 gtgagccgct gggctcagat ccacctggtg aagacaccct tggtgcagga ggtgcaccag
     5521 aacttctcag cttggtgttc ccaggtggtt cgcctgtacc caggacagcg gcacctggag
     5581 ctagagtggt cggtggggcc gatacctgtg ggcgacacct gggggaagga ggtcatcagc
     5641 cgttttgaca caccgctgga gacaaaggga cgcttctaca cagacagcaa tggccgggag
     5701 atcctggaga ggaggcggga ttatcgaccc acctggaaac tgaaccagac ggagcccgtg
     5761 gcaggaaact actatccagt caacacccgg atttacatca cggatggaaa catgcagctg
     5821 actgtgctga ctgaccgctc ccaggggggc agcagcctga gagatggctc gctggagctc
     5881 atggtgcacc gaaggctgct gaaggacgat ggacgcggag tatcggagcc actaatggag
     5941 aacgggtcgg gggcgtgggt gcgagggcgc cacctggtgc tgctggacac agcccaggct
     6001 gcagccgccg gacaccggct cctggcggag caggaggtcc tggcccctca ggtggtgctg
     6061 gccccgggtg gcggcgccgc ctacaatctc ggggctcctc cgcgcacgca gttctcaggg
     6121 ctgcgcaggg acctgccgcc ctcggtgcac ctgctcacgc tggccagctg gggccccgaa
     6181 atggtgctgc tgcgcttgga gcaccagttt gccgtaggag aggattccgg acgtaacctg
     6241 agcgcccccg ttaccttgaa cttgagggac ctgttctcca ccttcaccat cacccgcctg
     6301 caggagacca cgctggtggc caaccagctc cgcgaggcag cctccaggct caagtggaca
     6361 acaaacacag gccccacacc ccaccaaact ccgtaccagc tggacccggc caacatcacg
     6421 ctggaaccca tggaaatccg cactttcctg gcctcagttc aatggaagga ggtggatggt
     6481 tagaacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt
     6541 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     6601 acgcgtaccg gttagtaatg agatccctcc ccccccccta acgttactgg ccgaagccgc
     6661 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     6721 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     6781 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     6841 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     6901 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     6961 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     7021 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     7081 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     7141 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     7201 acatatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     7261 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     7321 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     7381 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     7441 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     7501 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     7561 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     7621 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     7681 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     7741 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     7801 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     7861 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     7921 gtaaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7981 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     8041 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     8101 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     8161 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8221 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8281 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     8341 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8401 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8461 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8521 ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8581 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     8641 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8701 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8761 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8821 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8881 gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt
     8941 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     9001 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     9061 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     9121 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9181 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9241 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9301 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9361 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     9421 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9481 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9541 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9601 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9661 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9721 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9781 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9841 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9901 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9961 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    10021 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    10081 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10141 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10201 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10261 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10321 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10381 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10441 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10501 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10561 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10621 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10681 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10741 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10801 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10861 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10921 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10981 ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc
    11041 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    11101 aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg
    11161 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    11221 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    11281 gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    11341 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    11401 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    11461 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    11521 gttaaatatg tactacaaac ttagtagt
//
