LOCUS       sequence-241459-        3737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector Backbone for his-24 fusion and let-858 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3737)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS2-his-24
FEATURES             Location/Qualifiers
     source          1..3737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..55
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     47..64
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     48..64
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     74..93
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        74..92
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(997..1017)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(997..1015)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1036..1052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1036..1052)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1049..1071)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1060..1076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1084..1090,1091..1108,1109..1114))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1129..1150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1267..1284)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1438..2026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1518..1537)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2197..2988,2989..3057))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2820..2839
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3058..3162)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3188..3643)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3325..3346)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3537..3556
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
       61 cagtgagcgc gcgtaatacg actcactata gggcgaattg gagctccacc gcggactagt
      121 ggatcccccg ggctgcagcc taggatgtct gattccgctg ttgttgccgc cgctgtcgag
      181 ccaaaggtcc caaaggctaa ggccgccaag gccgccaagc cagccgccaa ggccgccaag
      241 ccaaccaaag ttgccaaggc caaggctcca gtcgctcacc caccatacat caacatgatc
      301 aaggaggcca tcaagcagct caaggaccgc aaaggagctt ccaagcaggc aatcctcaag
      361 ttcatctccc aaaactacaa gctcggagac aatgtcatcc agatcaatgc tcatctccgt
      421 caggctctca agcgtggtgt caccagcaag gcccttgttc aagccgccgg atccggagcc
      481 aacggacgtt tccgtgtgcc agagaaggcc gccgccgcca agaagccagc agcagccaag
      541 aagccagcag cagccaagaa accagcagcc gccaagaaag ccactggaga gaagaaggca
      601 aagaagccag ccgctgccaa gccaaagaag gccgccaccg gagataagaa ggtcaagaag
      661 gccaagagcc caaagaaggt tgccaagcca gctgccaaga aggtcgccaa gtctccagcc
      721 aagaaggccg ctccaaagaa gatcgccaag ccagctgcca agaaggccgc caagccagcc
      781 gccaaggccg catgcaagct ttggaattcg ctagccggcc atacaagtaa tccggatgat
      841 cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat
      901 tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa
      961 tctaaacatg aggggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
     1021 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1081 acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1141 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1201 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1261 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1321 tcacccaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1381 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1441 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1501 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1561 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1621 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1681 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1741 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1801 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1861 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1921 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1981 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2041 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2101 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2161 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2221 acctatctca gcgatctgcc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2281 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2341 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2401 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2461 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2521 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2581 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2641 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2701 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2761 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2821 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2881 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2941 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3001 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3061 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3121 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3181 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     3241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     3301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     3361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     3421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     3481 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     3541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     3601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     3661 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     3721 aaagggggat gtgctgc
//
