LOCUS       sequence-241464-        7324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HPV16 E6 TCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Draper LM, Kwong ML, Gros A, Stevanovic S, Tran E, Kerkar S, Raffeld
            M, Rosenberg SA, Hinrichs CS
  TITLE     Targeting of HPV-16+ Epithelial Cancer Cells by TCR Gene Engineered 
            T Cells Directed against E6.
  JOURNAL   Clin Cancer Res. 2015 Oct 1;21(19):4431-9. doi: 
            10.1158/1078-0432.CCR-14-3341.
  PUBMED    26429982
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/1078-0432";
            journalName: "Clin Cancer Res"; date: "2015-10-1- 1"; volume: "21"; 
            issue: "19"; pages: "4431-9"
COMMENT     Sequence Label: HPV16 E6 TCR
FEATURES             Location/Qualifiers
     source          1..7324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     788..810
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     826..842
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1180..1196
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2073..2129
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             3140..3654
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3826..3842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3850..3856,3857..3874,3875..3880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3895..3916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4033..4050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4204..4792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4284..4303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4963..5754,5755..5823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5586..5605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5824..5928)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5996..6014
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6052..6074)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6174..6193
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6387..6409
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6537..6556
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6756..7272
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 cgccgggagg taagctggcc agcggtcgtt tcgtgtctgt ctctgtcttt gtgcgtgttt
       61 gtgccggcat ctaatgtttg cgcctgcgtc tgtactagtt agctaactag ctctgtatct
      121 ggcggacccg tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc
      181 ccagggactt cgggggccgt ttttgtggcc cgacctgagt cctaaaatcc cgatcgttta
      241 ggactctttg gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac
      301 ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc
      361 gcgcgtcttg tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat
      421 ttgtctgaaa atatgggccc gggctagcct gttaccactc ccttaagttt gaccttaggt
      481 cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt
      541 tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc
      601 acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat
      661 ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct
      721 ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
      781 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
      841 actccttctc taggcgcccc catatggcca tatgagatct tatatggggc acccccgccc
      901 cttgtaaact tccctgaccc tgacatgaca agagttacta acagcccctc tctccaagct
      961 cacttacagg ctctctactt agtccagcac gaagtctgga gacctctggc ggcagcctac
     1021 caagaacaac tggaccgacc ggtggtacct cacccttacc gagtcggcga cacagtgtgg
     1081 gtccgccgac accagactaa gaacctagaa cctcgctgga aaggacctta cacagtcctg
     1141 ctgaccaccc ccaccgccct caaagtagac ggcatcgcag cttggataca cgccgcccac
     1201 gtgaaggctg ccgaccccgg gggtggacca tcctctagac cgccatggcc cttgaacatc
     1261 tgttgataat actgtggatg caacttacct gggtatctgg tcagcaattg aaccagtctc
     1321 cacagtccat gtttatccaa gaaggggagg acgtgtccat gaattgtaca agctccagta
     1381 tcttcaacac gtggctgtgg tacaaacagg atcctgggga aggaccagta ctgttgatag
     1441 ccctctataa ggcgggcgaa ctgacatcca atggaagact cacagcccaa ttcggaatta
     1501 cccggaagga ttctttcctc aacatctccg cgagtattcc atctgatgta ggaatatact
     1561 tctgtgcagg gcatccgtct agcaacagtg gatatgccct caattttgga aagggcacct
     1621 ccctgctggt taccccagac attcagaacc ccgaaccagc cgtatatcag ttgaaggacc
     1681 caagatctca ggatagtaca ctctgtttgt ttacggactt tgactcacaa atcaacgtcc
     1741 cgaagactat ggaaagtggt acgttcatca cagataagac ggttctggac atgaaggcta
     1801 tggactcaaa gagcaacggg gcaattgctt ggtccaacca gacaagcttt acctgtcagg
     1861 acatttttaa ggagactaat gctacttatc cctccagcga cgttccgtgt gatgcgactc
     1921 ttaccgagaa gtcttttgag accgatatga atctcaactt ccagaatctg tcagtgatgg
     1981 gtctgcggat cctgcttctg aaggttgcag gattcaatct tcttatgact ctccggctct
     2041 ggtcttcaag agccaaaaga agtggttctg gcgcgacgaa ttttagtttg cttaagcaag
     2101 ccggagatgt ggaggaaaat cctggaccga tgggcacaag tttgctgtgc tgggttgtgt
     2161 tgggctttct gggtacagat catactgggg cgggagtctc tcaaagcccc cgatacaaag
     2221 tcactaaaag agggcaggat gtcgcgcttc gctgtgatcc cattagcggg catgtctccc
     2281 tttattggta ccggcaggct ttgggacaag gaccggagtt cctcacttac ttcaactacg
     2341 aagcgcagca ggacaagtcc ggtctgccta atgatagatt cagcgccgag agaccggagg
     2401 gcagtatctc tactcttacg atacaaagaa cggagcagcg agactctgct atgtatagat
     2461 gtgcaagttc tagccagacg ggtgctcgca cggacactca atatttcggt cctggtacaa
     2521 gattgaccgt cttggaggat ctccggaacg tcaccccacc aaaggtcagt ttgtttgagc
     2581 catcaaaggc ggagatcgcc aacaaacaga aagctacgct cgtgtgtttg gctcggggct
     2641 tcttcccaga ccacgtagaa ctttcctggt gggtcaatgg aaaggaggtt cattccggag
     2701 tgtccactga tccccaagcg tacaaggaat ccaactatag ctactgtctc tcatctcggc
     2761 tccgggtgag tgcgacattc tggcataatc ctcggaacca ctttcgatgc caagtgcagt
     2821 ttcatgggtt gagcgaggaa gacaagtggc ccgagggcag tcctaaacca gtcactcaaa
     2881 acataagcgc cgaggcatgg ggtagagccg attgtgggat tactagcgct tcataccaac
     2941 aaggggtatt gagcgctaca attctttacg aaattctcct cggcaaggcg acgctctacg
     3001 ccgtactggt gtctactctc gtggttatgg caatggtgaa acggaaaaac agctaatgag
     3061 aattctgcag tcgacggtac cgcgggcccg ggatccgata aaataaaaga ttttatttag
     3121 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     3181 aacgccattt tgcaaggcat ggaaaataca taactgagaa tagagaagtt cagatcaagg
     3241 ttaggaacag agagacagca gaatatgggc caaacaggat atctgtggta agcagttcct
     3301 gccccggctc agggccaaga acagatggtc cccagatgcg gtcccgccct cagcagtttc
     3361 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     3421 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     3481 aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc
     3541 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
     3601 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatgggtaa
     3661 cagtttcttg aagttggaga acaacattct gagggtagga gtcgaatatt aagtaatcct
     3721 gactcaatta gccactgttt tgaatccaca tactccaata ctcctgaaat ccatcgatgg
     3781 agttcattat ggacagcgca gaaagagctg gggagaattg tgaaattgtt atccgctcac
     3841 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3901 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3961 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4621 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5041 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5281 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5581 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5821 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5941 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     6001 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     6061 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     6121 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     6181 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6241 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     6301 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     6361 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg
     6421 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
     6481 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
     6541 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     6601 cgtccggcgt agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct
     6661 ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa
     6721 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     6781 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag
     6841 agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc
     6901 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     6961 ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     7021 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     7081 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
     7141 gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg
     7201 aatcgtggac tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg
     7261 gggtctttca tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc
     7321 cccc
//
