LOCUS       sequence-241465-        7309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HPV16 E7 TCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Jin BY, Campbell TE, Draper LM, Stevanovic S, Weissbrich B, Yu Z, 
            Restifo NP, Rosenberg SA, Trimble CL, Hinrichs CS
  TITLE     Engineered T cells targeting E7 mediate regression of human 
            papillomavirus cancers in a murine model.
  JOURNAL   JCI Insight. 2018 Apr 19;3(8). pii: 99488. doi: 
            10.1172/jci.insight.99488.
  PUBMED    29669936
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2018-04-19"; volume: "3(8). pii: 99488. doi"; 
            pages: " 10.1172/jci.insight.99488"
COMMENT     Sequence Label: HPV16 E7 TCR
FEATURES             Location/Qualifiers
     source          1..7309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             949..1005
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             1881..2395
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    2567..2583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2591..2597,2598..2615,2616..2621))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2636..2657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2774..2791)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2945..3533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3025..3044)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3704..4495,4496..4564))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4327..4346
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4565..4669)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4737..4755
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4793..4815)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4915..4934
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5128..5150
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5278..5297
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             5497..6013
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     6853..6875
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6891..6907
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     7245..7261
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 atggccccgg ggcttttgtg ttgggccttg ctttgtttgc ttggggcagg cttggtggat
       61 gctggagtca cacagtcacc cacacacctc attaaaacca ggggacaaca agtcactctg
      121 cgctgcagtc ctaagtcagg ccatgacaca gtttcctggt atcaacaggc tctggggcag
      181 ggccctcagt tcattttcca atattacgag gaagaggaac gccaacgcgg taatttcccc
      241 gatcggttct ctgggcacca gttcccaaac tactcaagtg agttgaacgt aaatgctctc
      301 ctcctcggag actccgccct ctacttgtgt gccagttctc ttggttggcg gggcggccga
      361 tacaatgaac aattttttgg acctggtact cggctgaccg tgctagagga cctgcgcaac
      421 gtcaccccac caaaggtcag tttgtttgag ccatcaaagg cggagatcgc caacaaacag
      481 aaagctacgc tcgtgtgttt ggctcggggc ttcttcccag accacgtaga actttcctgg
      541 tgggtcaatg gaaaggaggt tcattccgga gtgtgcactg atccccaagc gtacaaggaa
      601 tccaactata gctactgtct ctcatctcgg ctccgggtga gtgcgacatt ctggcataat
      661 cctcggaacc actttcgatg ccaagtgcag tttcatgggt tgagcgagga agacaagtgg
      721 cccgagggca gtcctaaacc agtcactcaa aacataagcg ccgaggcatg gggtagagcc
      781 gattgtggga ttactagcgc ttcataccaa caaggggtat tgagcgctac aattctttac
      841 gaaattctcc tcggcaaggc gacgctctac gccgtactgg tgtctactct cgtggttatg
      901 gcaatggtga aacggaaaaa cagcagagcc aaaagaagtg gttctggcgc gacgaatttt
      961 agtttgctta agcaagccgg agatgtggag gaaaatcctg gaccgatgtg gggtgtcttc
     1021 cttttgtacg tcagcatgaa gatgggaggc actactgggc aaaacataga tcagcctacc
     1081 gaaatgactg ctaccgaggg agccattgtc caaatcaact gcacctatca gactagcggc
     1141 ttcaatggac tcttctggta ccaacagcat gcgggcgaag cacctacctt cttgtcctat
     1201 aatgtcttgg atggtctcga agagaaaggc agattctcca gtttcctcag ccggagcaag
     1261 ggatactcat atcttctcct gaaagagctt cagatgaagg attctgcatc ctatctctgt
     1321 gcttcagtcg atggcaataa ccgactcgcc tttggaaaag ggaatcaagt ggtcgtcata
     1381 ccgaatattc agaaccccga accagccgta tatcagttga aggacccaag atctcaggat
     1441 agtacactct gtttgtttac ggactttgac tcacaaatca acgtcccgaa gactatggaa
     1501 agtggtacgt tcatcacaga taagtgcgtt ctggacatga aggctatgga ctcaaagagc
     1561 aacggggcaa ttgcttggtc caaccagaca agctttacct gtcaggacat ttttaaggag
     1621 actaatgcta cttatccctc cagcgacgtt ccgtgtgatg cgactcttac cgagaagtct
     1681 tttgagaccg atatgaatct caacttccag aatctgctgg tgatcgttct gcggatcctg
     1741 cttctgaagg ttgcaggatt caatcttctt atgactctcc ggctctggtc ttcatgataa
     1801 gaattctgca gtcgacggta ccgcgggccc gggatccgat aaaataaaag attttattta
     1861 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     1921 taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag
     1981 gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc
     2041 tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt
     2101 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     2161 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     2221 caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc
     2281 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     2341 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatgggta
     2401 acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat taagtaatcc
     2461 tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa tccatcgatg
     2521 gagttcatta tggacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca
     2581 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2641 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2701 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2761 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2821 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2881 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2941 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3001 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3061 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3121 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3181 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3241 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3301 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3361 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3421 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3481 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3541 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3601 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3661 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3721 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3781 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3841 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3901 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3961 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4021 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4081 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4141 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4201 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4261 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4321 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4381 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4441 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4501 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4561 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4621 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4681 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4741 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     4801 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     4861 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     4921 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     4981 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     5041 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     5101 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggcgcaag
     5161 gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
     5221 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     5281 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     5341 gcgtccggcg tagaggcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc
     5401 tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa
     5461 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
     5521 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata
     5581 gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat
     5641 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     5701 cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     5761 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     5821 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc
     5881 cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
     5941 gaatcgtgga ctcgctgatc cttgggaggg tctcctcaga ttgattgact gcccacctcg
     6001 ggggtctttc atttggaggt tccaccgaga tttggagacc cctgcccagg gaccaccgac
     6061 ccccccgccg ggaggtaagc tggccagcgg tcgtttcgtg tctgtctctg tctttgtgcg
     6121 tgtttgtgcc ggcatctaat gtttgcgcct gcgtctgtac tagttagcta actagctctg
     6181 tatctggcgg acccgtggtg gaactgacga gttcggaaca cccggccgca accctgggag
     6241 acgtcccagg gacttcgggg gccgtttttg tggcccgacc tgagtcctaa aatcccgatc
     6301 gtttaggact ctttggtgca ccccccttag aggagggata tgtggttctg gtaggagacg
     6361 agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg gaccgaagcc
     6421 gcgccgcgcg tcttgtctgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc
     6481 tgtatttgtc tgaaaatatg ggcccgggct agcctgttac cactccctta agtttgacct
     6541 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     6601 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     6661 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     6721 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     6781 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     6841 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     6901 ccctcactcc ttctctaggc gcccccatat ggccatatga gatcttatat ggggcacccc
     6961 cgccccttgt aaacttccct gaccctgaca tgacaagagt tactaacagc ccctctctcc
     7021 aagctcactt acaggctctc tacttagtcc agcacgaagt ctggagacct ctggcggcag
     7081 cctaccaaga acaactggac cgaccggtgg tacctcaccc ttaccgagtc ggcgacacag
     7141 tgtgggtccg ccgacaccag actaagaacc tagaacctcg ctggaaagga ccttacacag
     7201 tcctgctgac cacccccacc gccctcaaag tagacggcat cgcagcttgg atacacgccg
     7261 cccacgtgaa ggctgccgac cccgggggtg gaccatcctc tagaccgcc
//
