LOCUS       sequence-24148-e        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme T59A WT*
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    725..741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(749..755,756..771,772..777))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    847..863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     gap             1092..1111
                     /estimated_length=20
ORIGIN
        1 gctantgaaa aagcccgctc attaggcggg ctcagatctg ctcatgtttg acagcttatc
       61 atcgataagc ttcggcaata aattcttgat atgttttcat atgtttcctt taaatatttt
      121 aattagtaat tgtctattca agtaattgtg aatatactat cacaattcca agagaaagta
      181 aacagcttta tagattttta tacgcgtccc aagtgccagt tctaaacgtt gtaatgactc
      241 gttttgcgcg attaggtgtt tgattatacc atctactttt agctaagtta actgctgctt
      301 catcccagcg tttttgttga agcatacgta aagagttagt aaatcctgcc acaccggttt
      361 ctcccatttg gaaaaccata ttaatcaatg cagcgcgacg aaccgcatca agagaatcat
      421 aaaccggttt taatttagca tttctcagaa ttccgcgaac agcagcatca acatcctgat
      481 taaagagttt ttcagcctca tctttagcaa ttacaccatt agtattacgc ccaatagctt
      541 tatctaattc agatttagca gcattaagtg atggactttt tgtaagcaaa tgaccgatgc
      601 caatagtgta atagccttct gtgtctttat agattttaag tctaagacct tcatctatac
      661 gtaacatttc aaatatattc ataatacctc ctaagcatcg atggatcctg tttcctgtgt
      721 gaaattgtta tccgctcaca attccacaca ttatacgagc cgatgattaa ttgtcaacag
      781 ggggatgggg agtaagctca tcgatgtgaa tccgtaatca tggncatagc tgtttcctgn
      841 gngaaattgt tatccgctca caattcccca cattanacna gccggaagcn naaagngnaa
      901 agccnggggg ngccnaangn gngngccnaa cttncnntaa ntgcgtngcn cncncggccc
      961 ncntttccnn ncggggaaan ccngncnngg ccnggncccc nncnnaaagg ggggcnaanc
     1021 ctttcncggn nnggnnnggn nnnnnccccg gnaanaaann nnnnnncnng ggggggggna
     1081 ngggnaaanc cnnnnnnnnn nnnnnnnnnn ncnnnnaann ngnc
//
