LOCUS       sequence-24151-e        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme T59S WT*
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(712..734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    723..739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(747..753,754..769,770..775))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        complement(join(873..879,880..897,898..903))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 ctangaaaaa gcccgctcat taggcgggct cagatctgct catgtttgac agcttatcat
       61 cgataagctt cggcaataaa ttcttgatat gttttcatat gtttccttta aatattttaa
      121 ttagtaattg tctattcaag taattgtgaa tatactatca caattccaag agaaagtaaa
      181 cagctttata gatttttata cgcgtcccaa gtgccagttc taaacgttgt aatgactcgt
      241 tttgcgcgat taggtgtttg attataccat ctacttttag ctaagttaac tgctgcttca
      301 tcccagcgtt tttgttgaag catacgtaaa gagttagtaa atcctgccac accggtttct
      361 cccatttgga aaaccatatt aatcaatgca gcgcgacgaa ccgcatcaag agaatcataa
      421 accggtttta atttagcatt tctcagaatt ccgcgaacag cagcatcaac atcctgatta
      481 aagagttttt cagcctcatc tttagaaatt acaccattag tattacgccc aatagcttta
      541 tctaattcag atttagcagc attaagtgat ggactttttg taagcaaatg accgatgcca
      601 atagtgtaat agccttctgt gtctttatag attttaagtc taagaccttc atctatacgt
      661 aacatttcaa atatattcat aatacctcct aagcatcgat ggatcctgtt tcctgtgtga
      721 aattgttatc cgctcacaat tccacacatt atacgagccg atgattaatt gtcaacaggg
      781 ggatggggag taagctcatc gatggtgaan ccggnaatca tgggncatag ctgnttcccg
      841 gggggaaatt gntatccgct cacaattccn cacattanac gagccggaag cataaagtgt
      901 aaagccnggg gtgcctaatg agtgagctan cttacattaa ttgcgttgcg ctcactgncc
      961 gctttccagt cgggnaancc ggtcgngcca ggacnccntc naanggggca aaccnttcnn
     1021 gggntggcnt ganancnccc nggaaaaaag tcnattncgg gggggggaag ggga
//
