LOCUS       sequence-24155-e        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme E64-[A] WT*
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    727..743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(751..757,758..773,774..779))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    822..838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(846..852,853..868,869..874))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     gap             1004..1019
                     /estimated_length=16
     gap             1038..1049
                     /estimated_length=12
     gap             1068..1079
                     /estimated_length=12
     gap             1082..1094
                     /estimated_length=13
ORIGIN
        1 gctangaaaa gcccgctcat taggcgggct cagatctgct catgttttga cagcttatca
       61 tcgataagct tcggcaataa attcttgata tgttttcata tgtttccttt aaatatttta
      121 attagtaatt gtctattcaa gtaattgtga atatactatc acaattccaa gagaaagtaa
      181 acagctttat agatttttat acgcgtccca agtgccagtt ctaaacgttg taatgactcg
      241 ttttgcgcga ttaggtgttt gattatacca tctactttta gctaagttaa ctgctgcttc
      301 atcccagcgt ttttgttgaa gcatacgtaa agagttagta aatcctgcca caccggtttc
      361 tcccatttgg aaaaccatat taatcaatgc agcgcgacga accgcatcaa gagaatcata
      421 aaccggtttt aatttagcat ttctcagaat tccgcgaaca gcagcatcaa catcctgatt
      481 aaagagtttt gcttcagcct catcttttgt aattacacca ttagtattac gcccaatagc
      541 tttatctaat tcagatttag cagcattaag tgatggactt tttgtaagca aatgaccgat
      601 gccaatagtg taatagcctt ctgtgtcttt atagatttta agtctaagac cttcatctat
      661 acgtaacatt tcaaatatat tcataatacc tcctaagcat cgatggatcc tgtttcctgt
      721 gtgaaattgt tatccgctca caattccaca cattatacga gccgatgatt aattgtcaac
      781 agggggatgg ggagtaagct gatcctgttt cctgtgtgaa attgttatcc gctcacaatt
      841 ccacacatta tacgagccga tgattaattg tcaacagggg gatggggagt aagctcatcg
      901 atggatcgat ccggnttccn gggggnaant gnnnnccgcn cnnantccnc nnnnnnnncn
      961 anccgnaann nnnannngnn anncnngggg ggccnnnngn nngnnnnnnn nnnnnnnnng
     1021 gnnnngnnnn nnngnccnnn nnnnnnnnng nnannnnnnn nggnnngnnn nnnnnnnnng
     1081 gnnnnnnnnn nnnng
//
