LOCUS       sequence-24161-e        1105 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1105)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme N68C A93C WT*
FEATURES             Location/Qualifiers
     source          1..1105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    719..735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(743..749,750..765,766..771))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    814..830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(838..844,845..860,861..866))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 angaaagccc gctcattagg cgggctcaga tctgctcatg tttgacagct tatcatcgat
       61 aagcttcggc aataaattct tgatatgttt tcatatgttt cctttaaata ttttaattag
      121 taattgtcta ttcaagtaat tgtgaatata ctatcacaat tccaagagaa agtaaacagc
      181 tttatagatt tttatacgcg tcccaagtgc cagttctaaa cgttgtaatg actcgttttg
      241 cgcgattagg tgtttgatta taccatctac ttttagctaa gttaactgct gcttcatccc
      301 agcgtttttg ttgaagcata cgtaaagagt tagtaaatcc tgccacaccg gtttctccca
      361 tttggaaaac catattaatc aatgcagcgc gacgaacaca atcaagagaa tcataaaccg
      421 gttttaattt agcatttctc agaattccgc gaacagcagc atcaacatcc tgacaaaaga
      481 gtttttcagc ctcatctttt gtaattacac cattagtatt acgcccaata gctttatcta
      541 attcagattt agcagcatta agtgatggac tttttgtaag caaatgaccg atgccaatag
      601 tgtaatagcc ttctgtgtct ttatagattt taagtctaag accttcatct atacgtaaca
      661 tttcaaatat attcataata cctcctaagc atcgatggat cctgtttcct gtgtgaaatt
      721 gttatccgct cacaattcca cacattatac gagccgatga ttaattgtca acagggggat
      781 ggggagtaag ctgatcctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat
      841 tatacgagcc gatgattaat tgtcaacagg ggggatgggg gagtaagctc atcgatggat
      901 cgatccggnt tncctggggn gaaaatggtt atccgctccc caattccccc acnattatac
      961 gagccgggaa gccataaagg ggnnaagccn ggggggngcc taangngnga gcnnacntta
     1021 cnttaattgg ggttgcggcc nccnggcccn ctttccantc gggaaanccg gncgggnccn
     1081 ggnncccntn nnangggggc naaac
//
