LOCUS       sequence-24165-e        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme D72P WT*
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    717..733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(741..747,748..763,764..769))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    812..828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(836..842,843..858,859..864))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gaaaagccgc tcattaggcg ggctcagatc tgctcatgtt tgacagctta tcatcgataa
       61 gcttcggcaa taaattcttg atatgttttc atatgtttcc tttaaatatt ttaattagta
      121 attgtctatt caagtaattg tgaatatact atcacaattc caagagaaag taaacagctt
      181 tatagatttt tatacgcgtc ccaagtgcca gttctaaacg ttgtaatgac tcgttttgcg
      241 cgattaggtg tttgattata ccatctactt ttagctaagt taactgctgc ttcatcccag
      301 cgtttttgtt gaagcatacg taaagagtta gtaaatcctg ccacaccggt ttctcccatt
      361 tggaaaacca tattaatcaa tgcagcgcga cgaaccgcat caagagaatc ataaaccggt
      421 tttaatttag catttctcag aattccgcga acagcagcag gaacatcctg attaaagagt
      481 ttttcagcct catcttttgt aattacacca ttagtattac gcccaatagc tttatctaat
      541 tcagatttag cagcattaag tgatggactt tttgtaagca aatgaccgat gccaatagtg
      601 taatagcctt ctgtgtcttt atagatttta agtctaagac cttcatctat acgtaacatt
      661 tcaaatatat tcataatacc tcctaagcat cgatggatcc tgtttcctgt gtgaaattgt
      721 tatccgctca caattccaca cattatacga gccgatgatt aattgtcaac agggggatgg
      781 ggagtaagct gatcctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacatta
      841 tacgagccga tgattaattg tcaacagggg gatggggagt aagctcatcg atggatcgac
      901 cgggnttncc cgggggggaa aanggtnanc cgcncccaat tccncncatt tatacgagcc
      961 ggaagcataa agtgnaaagc ccgggggngc ctaangngng gagctaactt acattaattg
     1021 cgttgngctc ncngnccnct ttccgnncgg gaaaccngnc nggccnggan ncc
//
