LOCUS       sequence-241685-       11665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of WNK1__PRMT8 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-WNK1__PRMT8
FEATURES             Location/Qualifiers
     source          1..11665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5584..5608)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6725..6745)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6799..6840
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6861..7428
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6878..7430
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7022..7039)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7249..7268
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7442..8041
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7442..7461)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7938..7958
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8055..8643
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8108..8128)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8526..8537)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8718..8951
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9070..9088)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9156..9260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9261..9329,9330..10121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9479..9498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10292..10880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10781..10800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11034..11051
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11291..11312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11327..11332,11333..11350,11351..11357)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11365..11381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11370..11392
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11389..11405
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11389..11405
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11455..11474
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgtct ggcggcgccg cagagaagca gagcagcact
     3481 cccggttccc tgttcctctc gccgccggct cctgccccca agaatggctc cagctccgat
     3541 tcctccgtgg gggagaaact gggagccgcg gccgccgacg ctgtgaccgg caggaccgag
     3601 gagtacaggc gccgccgcca cactatggac aaggacagcc gtggggcggc cgcgaccact
     3661 accaccactg agcaccgctt cttccgccgg agcgtcatct gtgactccaa tgccactgca
     3721 ctggagcttc ccggccttcc tctttccctg ccccagccca gcatccccgc ggctgtcccg
     3781 cagagtgctc caccggagcc ccaccgggaa gagaccgtga ccgccaccgc cacttcccag
     3841 gtagcccagc agcctccagc cgctgccgcc cctggggaac aggccgtcgc gggccctgcc
     3901 ccctcgactg tccccagcag taccagcaaa gaccgcccag tgtcccagcc tagccttgtg
     3961 gggagcaaag aggagccgcc gccggcgaga agtggcagcg gcggcggcag cgccaaggag
     4021 ccacaggagg aacggagcca gcagcaggat gatatcgaag agctggagac caaggccgtg
     4081 ggaatgtcta acgatggccg ctttctcaag tttgacatcg aaatcggcag aggctccttt
     4141 aagacggtct acaaaggtct ggacactgaa accaccgtgg aagtcgcctg gtgtgaactg
     4201 caggatcgaa aattaacaaa gtctgagagg cagagattta aagaagaagc tgaaatgtta
     4261 aaaggtcttc agcatcccaa tattgttaga ttttatgatt cctgggaatc cacagtaaaa
     4321 ggaaagaagt gcattgtttt ggtgactgaa cttatgacgt ctggaacact taaaacgtat
     4381 ctgaaaaggt ttaaagtgat gaagatcaaa gttctaagaa gctggtgccg tcagatcctt
     4441 aaaggtcttc agtttcttca tactcgaact ccacctatca ttcaccgcga tcttaaatgt
     4501 gacaacatct ttatcaccgg ccctactggc tcagtcaaga ttggagacct cggtctggca
     4561 accctgaagc gggcttcttt tgccaagagt gtgataggta ccccagagtt catggcccct
     4621 gagatgtatg aggagaaata tgatgaatcc gttgacgttt atgcttttgg gatgtgcatg
     4681 cttgagatgg ctacatctga atatccttac tcggagtgcc aaaatgctgc acagatctac
     4741 cgtcgcgtga ccagtggggt gaagccagcc agttttgaca aagtagcaat tcctgaagtg
     4801 aaggaaatta ttgaaggatg catacgacaa aacaaagatg aaagatattc catcaaagac
     4861 cttttgaacc atgccttctt ccaagaggaa acaggagtac gggtagaatt agcagaagaa
     4921 gatgatggag aaaaaatagc cataaaatta tggctacgta ttgaagatat taagaaatta
     4981 aagggaaaat acaaagataa tgaagctatt gagttttctt ttgatttaga gagagatgtc
     5041 ccagaagatg ttgcacaaga aatggtagag tctgggtatg tctgtgaagg tgatcacaag
     5101 accatggcta aagctatcaa agacagagta tcattaatta agaggaaacg agagcagcgg
     5161 cagttggtac gggaggagca agaaaaaaaa aagcaggaag agagcagtct caaacagcag
     5221 gtagaacaat ccagtgcttc ccagacagga atcaagcagc tcccttctgc tagcaccggc
     5281 atacctactg cttctaccac ttcagcttca gtttctacac aagtagaacc tgaagaacct
     5341 gaggcagatc aacatcaaca actacagtac cagcaaccca gtatatctgt gttatctgat
     5401 gggacggttg acagtggtca gggatcctct gtcttcacag aatctcgagt gagcagccaa
     5461 cagacagttt catatggttc ccaacatgaa caggcacatt ctacaggcac agtcccaggg
     5521 catatacctt ctactgtcca agcacagtct cagccccatg gggtatatcc accctcaagt
     5581 gtgtcccagc tttcttgtac aaagtggttc gtgaacagcc ccccctccca gcccccccag
     5641 cccgtcgtcc ctgctaagcc cgtgcaatgc gtccatcatg tgtccactca acccagctgc
     5701 ccaggacggg gcaagatgtc caagctgctg aacccagagg agatgacctc gagagattat
     5761 tacttcgact cctatgccca ctttgggatc cacgaggaaa tgctgaagga tgaggtgcgg
     5821 actctcactt accggaactc catgtaccac aacaagcacg tgttcaagga caaagtggta
     5881 ctggatgtgg ggagtggtac tgggatcctt tccatgttcg ctgccaaggc aggggccaag
     5941 aaggtgtttg ggatcgaatg ctccagtatt tctgactact cacagaagat cattaaggcc
     6001 aaccacttgg acaacatcat caccatattt aagggtaaag tggaagaggt ggagctgcct
     6061 gtggagaagg tggacatcat catcagcgag tggatgggct actgtctgtt ctatgagtcc
     6121 atgctcaaca cggtgatctt tgccagggac aagtggctga aacctggagg gcttatgttt
     6181 ccagaccggg cagctttgta cgtggtagcg attgaagaca gacagtacaa ggacttcaaa
     6241 atccactggt gggagaatgt ctatggcttt gacatgacct gcatcaggga cgtggccatg
     6301 aaggagcctc tagtggacat cgtggatcca aagcaagtgg tgaccaatgc ctgtttgata
     6361 aaggaggtgg acatttacac agtgaagacg gaagagctat cgttcacatc tgcattctgc
     6421 ctgcagatac agcgcaacga ctacgtccac gccctggtca cctattttaa tattgaattt
     6481 accaagtgcc acaagaaaat ggggttttcc acagcccctg atgctcccta cacccactgg
     6541 aagcagaccg tcttctactt ggaagattac ctcactgtcc ggagggggga ggaaatctac
     6601 gggaccatat ccatgaagcc aaatgccaaa aatgtgcgag acctcgattt cacagtagac
     6661 ttggatttta agggacagct gtgtgaaaca tctgtatcta atgactacaa aatgcgttag
     6721 cacccaactt ttctatacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta
     6781 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     6841 cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg aagccgcttg
     6901 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     6961 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     7021 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     7081 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     7141 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     7201 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     7261 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     7321 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     7381 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaca
     7441 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7501 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     7561 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     7621 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     7681 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7741 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7801 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     7861 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7921 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7981 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta
     8041 aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     8101 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     8161 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     8221 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     8281 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     8341 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     8401 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     8461 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     8521 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     8581 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     8641 cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8701 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     8761 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8821 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8881 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8941 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     9001 atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa
     9061 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     9121 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9181 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9241 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9301 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9361 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9421 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     9481 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     9541 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9601 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9661 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9721 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9781 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9841 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9901 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9961 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10021 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10081 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10141 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10201 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10261 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10321 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10381 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10441 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10501 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10561 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10621 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10681 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10741 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10801 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10861 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10921 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10981 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    11041 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    11101 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
    11161 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    11221 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    11281 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    11341 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    11401 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11461 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    11521 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    11581 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    11641 aaatatgtac tacaaactta gtagt
//
