LOCUS       sequence-241687-        4541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Nuclear Localized tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4541)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS2-his-24::tagBFP
FEATURES             Location/Qualifiers
     source          1..4541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..146
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     138..155
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     139..155
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     165..184
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        165..183
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1892..1912)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1892..1910)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1931..1947)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1931..1947)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1944..1966)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1955..1971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1979..1985,1986..2003,2004..2009))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2024..2045
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2162..2179)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2333..2921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2413..2432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3092..3883,3884..3952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3715..3734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3953..4057)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4083..4538)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4220..4241)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4432..4451
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
       61 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      121 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
      181 agggcgaatt ggagctccac cgcggactag tggatccccc gggctgcagc ctaggatgtc
      241 tgattccgct gttgttgccg ccgctgtcga gccaaaggtc ccaaaggcta aggccgccaa
      301 ggccgccaag ccagccgcca aggccgccaa gccaaccaaa gttgccaagg ccaaggctcc
      361 agtcgctcac ccaccataca tcaacatgat caaggaggcc atcaagcagc tcaaggaccg
      421 caaaggagct tccaagcagg caatcctcaa gttcatctcc caaaactaca agctcggaga
      481 caatgtcatc cagatcaatg ctcatctccg tcaggctctc aagcgtggtg tcaccagcaa
      541 ggcccttgtt caagccgccg gatccggagc caacggacgt ttccgtgtgc cagagaaggc
      601 cgccgccgcc aagaagccag cagcagccaa gaagccagca gcagccaaga aaccagcagc
      661 cgccaagaaa gccactggag agaagaaggc aaagaagcca gccgctgcca agccaaagaa
      721 ggccgccacc ggagataaga aggtcaagaa ggccaagagc ccaaagaagg ttgccaagcc
      781 agctgccaag aaggtcgcca agtctccagc caagaaggcc gctccaaaga agatcgccaa
      841 gccagctgcc aagaaggccg ccaagccagc cgccaaggcc gcatgcatgt ccgaactcat
      901 caaggagaac atgcacatga agctctacat ggagggaacc gtagacaacc accacttcaa
      961 gtgcacctcc gagggagagg gaaagccata cgagggaacc caaaccatgc gtatcaaggt
     1021 cgtcgaggga ggaccactcc cattcgcctt cgacatcctc gccacctcct tcctctacgg
     1081 gtccaaggta agtttaaaca tatatatact aactaaccct gattatttaa attttcagac
     1141 cttcatcaac cacacccaag gaatcccaga cttcttcaag caatccttcc cagagggatt
     1201 cacctgggag cgtgtcacca cctacgagga cggaggagtc ctcaccgcca cccaagacac
     1261 ctccctccaa gacggatgcc tcatctacaa cgtcaagatc cgtggagtca acttcacctc
     1321 caacggacca gtcatgcaaa agaagaccct cggatgggag accttcaccg agaccctcta
     1381 cccagccgac ggaggactag agggacgtaa cgacatggcc ctcaagctcg tcggaggctc
     1441 ccacctcatc gccaacatca aggtaagttt aaacagttcg gtactaacta accatacata
     1501 tttaaatttt cagaccacct accgttccaa gaagccagcc aagaacctca agatgccagg
     1561 agtctactac gttgactacc gtctagagcg tatcaaggag gccaacaacg agacctacgt
     1621 cgagcaacac gaggtcgccg tcgcccgtta ctgcgacctc ccatccaagc tcggacacaa
     1681 gctcaactaa aagctttgga attcgctagc cggccataca agtaatccgg atgatcgacg
     1741 ccaacgtcgt tgaattttca aattttaaat actgaatatt tgtttttttt cctattattt
     1801 atttattctc tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa
     1861 acatgagggg cccggtaccc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     1921 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1981 acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2041 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2101 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2161 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcacc
     2221 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3121 tctcagcgat ctgcctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4081 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4141 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     4201 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4261 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4321 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4381 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4441 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     4501 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc c
//
