LOCUS       sequence-241688-        5105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Nuclear Localized tagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5105)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS1-his-24::tagBFP2
FEATURES             Location/Qualifiers
     source          1..5105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..75
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     67..84
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     68..84
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     94..113
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        94..112
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2385..2405)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2385..2403)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2424..2440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2424..2440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2437..2459)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2448..2464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2472..2478,2479..2496,2497..2502))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2517..2538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2655..2672)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2826..3414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2906..2925)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3585..4376,4377..4445))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4208..4227
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4446..4550)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4576..5031)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4713..4734)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4925..4944
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
       61 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
      121 gagctccacc gcggactagt ggatcccccg ggctgcagcc taggatgtct gattccgctg
      181 ttgttgccgc cgctgtcgag ccaaaggtcc caaaggctaa ggccgccaag gccgccaagc
      241 cagccgccaa ggccgccaag ccaaccaaag ttgccaaggc caaggctcca gtcgctcacc
      301 caccatacat caacatgatc aaggaggcca tcaagcagct caaggaccgc aaaggagctt
      361 ccaagcaggc aatcctcaag ttcatctccc aaaactacaa gctcggagac aatgtcatcc
      421 agatcaatgc tcatctccgt caggctctca agcgtggtgt caccagcaag gcccttgttc
      481 aagccgccgg atccggagcc aacggacgtt tccgtgtgcc agagaaggcc gccgccgcca
      541 agaagccagc agcagccaag aagccagcag cagccaagaa accagcagcc gccaagaaag
      601 ccactggaga gaagaaggca aagaagccag ccgctgccaa gccaaagaag gccgccaccg
      661 gagataagaa ggtcaagaag gccaagagcc caaagaaggt tgccaagcca gctgccaaga
      721 aggtcgccaa gtctccagcc aagaaggccg ctccaaagaa gatcgccaag ccagctgcca
      781 agaaggccgc caagccagcc gccaaggccg catgcatgtc cgaactcatc aaggagaaca
      841 tgcacatgaa gctctacatg gagggaaccg tagacaacca ccacttcaag tgcacctccg
      901 agggagaggg aaagccatac gagggaaccc aaaccatgcg tatcaaggtc gtcgagggag
      961 gaccactccc attcgccttc gacatcctcg ccacctcctt cctctacggg tccaaggtaa
     1021 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagacc ttcatcaacc
     1081 acacccaagg aatcccagac ttcttcaagc aatccttccc agagggattc acctgggagc
     1141 gtgtcaccac ctacgaggac ggaggagtcc tcaccgccac ccaagacacc tccctccaag
     1201 acggatgcct catctacaac gtcaagatcc gtggagtcaa cttcacctcc aacggaccag
     1261 tcatgcaaaa gaagaccctc ggatgggaga ccttcaccga gaccctctac ccagccgacg
     1321 gaggactaga gggacgtaac gacatggccc tcaagctcgt cggaggctcc cacctcatcg
     1381 ccaacgccaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
     1441 agaccaccta ccgttccaag aagccagcca agaacctcaa gatgccagga gtctactacg
     1501 ttgactaccg tctagagcgt atcaaggagg ccaacaacga gacctacgtc gagcaacacg
     1561 aggtcgccgt cgcccgttac tgcgacctcc catccaagct cggacacaag ctcaactaaa
     1621 agctttggaa ttcatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca
     1681 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
     1741 tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta
     1801 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
     1861 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
     1921 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     1981 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     2041 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     2101 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg
     2161 tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     2221 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     2281 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     2341 tataccaaac ataactgttt gggcccggta cccagctttt gttcccttta gtgagggtta
     2401 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2461 acaattccac acaacatacg agccgggagc ataaagtgta aagcctgggg tgcctaatga
     2521 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2581 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2641 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2701 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2761 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3241 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3301 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3601 agtgaggcac ctatctcagc gatctgccta tttcgttcat ccatagttgc ctgactcccc
     3661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3901 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4201 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4561 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     4621 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     4681 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4741 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     4801 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     4861 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     4921 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     4981 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     5041 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     5101 agctg
//
