LOCUS       sequence-24169-e        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  TITLE     T4 Lysozyme Plasmids - Tray 2
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme A73-delta WT*
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    718..734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(742..748,749..764,765..770))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    813..829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(837..843,844..859,860..864))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctnggaaaag ccgctcatta ggcgggctca gatctgctca tgtttgacag cttatcatcg
       61 ataagcttcg gcaataaatt cttgatatgt tttcatatgt ttcctttaaa tattttaatt
      121 agtaattgtc tattcaagta attgtgaata tactatcaca attccaagag aaagtaaaca
      181 gctttataga tttttatacg cgtcccaagt gccagttcta aacgttgtaa tgactcgttt
      241 tgcgcgatta ggtgtttgat tataccatct acttttagct aagttaactg ctgcttcatc
      301 ccagcgtttt tgttgaagca tacgtaaaga gttagtaaat cctgccacac cggtttctcc
      361 catttggaaa accatattaa tcaatgcagc gcgacgaacc gcatcaagag aatcataaac
      421 cggttttaat ttagcatttc tcagaattcc gcgaacagca tcaacatcct gattaaagag
      481 tttttcagcc tcatcttttg taattacacc attagtatta cgcccaatag ctttatctaa
      541 ttcagattta gcagcattaa gtgatggact ttttgtaagc aaatgaccga tgccaatagt
      601 gtaatagcct tctgtgtctt tatagatttt aagtctaaga ccttcatcta tacgtaacat
      661 ttcaaatata ttcataatac ctcctaagca tcgatggatc ctgtttcctg tgtgaaattg
      721 ttatccgctc acaattccac acattatacg agccgatgat taattgtcaa cagggggatg
      781 gggagtaagc tgatcctgtt tcctgtgtga aattgttatc cgctcacaat tccacacatt
      841 atacgagccg atgattaatt gtcancaggg ggatggggga gtangctcat cgatggatcg
      901 atccggnttn ccgggggnaa atggtanccn cncncaattc cncncattat acgagccgga
      961 agctaaaggg naagccnggg ggggccnang ngggagcnaa cttannttaa tngngntgng
     1021 cncncngccc nctttccngn cgggaanccg gncgggccgg ganccnncnn aagggggcaa
     1081 ancnttncng ggna
//
