LOCUS       sequence-241690-        4541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Nuclear Localized tagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4541)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS2-his-24::tagBFP2
FEATURES             Location/Qualifiers
     source          1..4541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     69..91
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     83..100
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     84..100
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     110..129
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        110..128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1837..1857)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1837..1855)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1876..1892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1876..1892)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1889..1911)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1900..1916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1924..1930,1931..1948,1949..1954))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1969..1990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2107..2124)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2278..2866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2358..2377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3037..3828,3829..3897))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3660..3679
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3898..4002)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4028..4483)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4165..4186)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4377..4396
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
       61 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
      121 actatagggc gaattggagc tccaccgcgg actagtggat cccccgggct gcagcctagg
      181 atgtctgatt ccgctgttgt tgccgccgct gtcgagccaa aggtcccaaa ggctaaggcc
      241 gccaaggccg ccaagccagc cgccaaggcc gccaagccaa ccaaagttgc caaggccaag
      301 gctccagtcg ctcacccacc atacatcaac atgatcaagg aggccatcaa gcagctcaag
      361 gaccgcaaag gagcttccaa gcaggcaatc ctcaagttca tctcccaaaa ctacaagctc
      421 ggagacaatg tcatccagat caatgctcat ctccgtcagg ctctcaagcg tggtgtcacc
      481 agcaaggccc ttgttcaagc cgccggatcc ggagccaacg gacgtttccg tgtgccagag
      541 aaggccgccg ccgccaagaa gccagcagca gccaagaagc cagcagcagc caagaaacca
      601 gcagccgcca agaaagccac tggagagaag aaggcaaaga agccagccgc tgccaagcca
      661 aagaaggccg ccaccggaga taagaaggtc aagaaggcca agagcccaaa gaaggttgcc
      721 aagccagctg ccaagaaggt cgccaagtct ccagccaaga aggccgctcc aaagaagatc
      781 gccaagccag ctgccaagaa ggccgccaag ccagccgcca aggccgcatg catgtccgaa
      841 ctcatcaagg agaacatgca catgaagctc tacatggagg gaaccgtaga caaccaccac
      901 ttcaagtgca cctccgaggg agagggaaag ccatacgagg gaacccaaac catgcgtatc
      961 aaggtcgtcg agggaggacc actcccattc gccttcgaca tcctcgccac ctccttcctc
     1021 tacgggtcca aggtaagttt aaacatatat atactaacta accctgatta tttaaatttt
     1081 cagaccttca tcaaccacac ccaaggaatc ccagacttct tcaagcaatc cttcccagag
     1141 ggattcacct gggagcgtgt caccacctac gaggacggag gagtcctcac cgccacccaa
     1201 gacacctccc tccaagacgg atgcctcatc tacaacgtca agatccgtgg agtcaacttc
     1261 acctccaacg gaccagtcat gcaaaagaag accctcggat gggagacctt caccgagacc
     1321 ctctacccag ccgacggagg actagaggga cgtaacgaca tggccctcaa gctcgtcgga
     1381 ggctcccacc tcatcgccaa cgccaaggta agtttaaaca gttcggtact aactaaccat
     1441 acatatttaa attttcagac cacctaccgt tccaagaagc cagccaagaa cctcaagatg
     1501 ccaggagtct actacgttga ctaccgtcta gagcgtatca aggaggccaa caacgagacc
     1561 tacgtcgagc aacacgaggt cgccgtcgcc cgttactgcg acctcccatc caagctcgga
     1621 cacaagctca actaaaagct ttggaattcg ctagccggcc atacaagtaa tccggatgat
     1681 cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat
     1741 tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa
     1801 tctaaacatg aggggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
     1861 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1921 acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1981 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2041 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2101 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2161 tcacccaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2221 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2281 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2341 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2401 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2461 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2521 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2581 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2641 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2701 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2761 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2821 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2881 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2941 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3001 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3061 acctatctca gcgatctgcc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3901 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4021 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4081 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4141 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4201 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4261 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4321 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     4381 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4441 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     4501 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt c
//
