LOCUS       sequence-241694-        7535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Exogenous expression of PSD95 (red fluorescence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff 
            SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man HY, Han X
  TITLE     A Viral Toolbox of Genetically Encoded Fluorescent Synaptic Tags.
  JOURNAL   iScience. 2020 Jun 30;23(7):101330. doi: 10.1016/j.isci.2020.101330.
  PUBMED    32674057
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-06-30"; volume: "23(7):101330. doi"; pages: " 
            10.1016/j.isci.2020.101330"
COMMENT     Sequence Label: AAV-Syn-PSD95-mCherry
FEATURES             Location/Qualifiers
     source          1..7535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   380..509
                     /label=AAV2 ITR
     protein_bind    575..586
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        614..1061
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1131..1160
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3321..4031
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3466..3484)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3734..3753
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4044..4632
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4097..4117)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4515..4526)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4635..4651)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4636..4652)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4681..4802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4692..4711)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4746..4765
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4964..5093
                     /label=AAV2 ITR
     primer_bind     complement(5112..5129)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5112..5128)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5121..5143)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5270..5725
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5357..5376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5567..5588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5751..5855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5856..5924,5925..6716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6074..6093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6887..7475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7376..7395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaagatcac gcgtgtcatc ctcatcagat ctctgcagag
      601 ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat gaggcggggt gggggtgcct
      661 acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc
      721 atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag
      781 cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca
      841 gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt cccggccacc
      901 ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag
      961 gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg
     1021 cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgaattca agctgctagc
     1081 aaggatccgc caccatggac tgtctctgta tagtgacaac ccggcgcgcc gagcaaaagc
     1141 tgatatctga agaggacttg gggcgcgcca agaaataccg ctaccaagat gaagacacgc
     1201 cccctctgga acacagcccg gcccacctcc ccaaccaggc caattctccc cctgtgattg
     1261 tcaacacgga caccctagaa gccccaggat atgagttgca ggtgaatgga acagaggggg
     1321 agatggagta tgaggagatc acattggaaa ggggtaactc aggtctgggc ttcagcatcg
     1381 caggtggcac tgacaacccg cacatcggtg acgacccgtc catttttatc accaagatca
     1441 ttcctggtgg ggctgcagcc caggatggcc gcctcagggt caatgacagc atcctgtttg
     1501 taaatgaagt ggatgttcgg gaggtgaccc attcagctgc ggtggaggcc ctcaaagagg
     1561 caggttccat cgttcgccac tatgtcatgc gccggaaacc cccagccgaa aaggtcatgg
     1621 agatcaaact catcaaaggg cctaaaggac ttggcttcag cattgcgggg ggcgttggga
     1681 accagcacat ccctggagat aacagcatct atgtaacgaa gatcatcgaa ggaggtgctg
     1741 ctcacaagga tggcaggttg cagattggag acaagatcct ggcggtcaac agtgtggggc
     1801 tggaggacgt tatgcacgag gatgccgtgg cagccctgaa gaacacatat gacgttgtgt
     1861 acctaaaggt ggccaagccc agcaatgcct acctgagtga cagctatgct cccccagaca
     1921 tcacaacctc gtattctcag cacctggaca atgagatcag tcatagcagc tacttgggca
     1981 ctgactaccc cacagccatg acccccactt cccctcggcg ctactcccct gtggccaagg
     2041 acctgctggg ggaggaagac attccccggg aaccaaggcg gatcgtgatc catcggggct
     2101 ccaccggcct gggcttcaac atcgtgggcg gcgaggatgg tgaaggcatc ttcatctcct
     2161 tcatccttgc tgggggtcca gccgacctca gtggggagct acggaagggg gaccagatcc
     2221 tgtcggtcaa tggtgttgac ctccgcaatg ccagtcacga acaggctgcc attgccctga
     2281 agaatgcggg tcagacggtc acgatcatcg ctcagtataa accagaagag tatagtcgat
     2341 tcgaggccaa gatccatgat cttcgggaac agctcatgaa tagtagccta ggctcaggga
     2401 ctgcatcctt gcgaagcaac cccaagaggg gcttctacat tagggccctg tttgattacg
     2461 acaagaccaa ggactgcggt ttcttgagcc aggccctgag cttccgcttc ggggatgtgc
     2521 ttcatgtcat tgacgttggt gacgaagagt ggtggcaagc acggcgggtc cactccgaca
     2581 gtgagaccga cgacattggc ttcattccca gcaaacggcg ggtcgagcga cgagagtggt
     2641 caaggttaaa ggccaaggac tggggctcca gctctggatc acagggtcga gaagactcgg
     2701 ttctgagcta tgagacggtg acccagatgg aagtgcacta tgctcgtccc atcatcatcc
     2761 ttggacccac caaagaccgt gccaacgatg atcttctctc cgagttcccc gacaagtttg
     2821 gatcctgtgt ccctcatacg acacgtccta agcgggaata tgagatagac ggccaggatt
     2881 accactttgt ctcctcccgg gagaaaatgg agaaggacat ccaggcacac aagttcattg
     2941 aggctggcca gtacaacagc cacctctatg ggaccagcgt ccagtctgtg cgagaggtag
     3001 cagagcaggg gaagcactgc atcctcgatg tctcggccaa tgccgtgcgg cggctgcagg
     3061 cggcccacct gcaccccatc gccatcttca tccgtccccg ctccctggag aatgtgctag
     3121 agatcaataa gcggatcaca gaggagcaag cccggaaagc cttcgacaga gccacgaagc
     3181 tggagcagga gttcacagag tgcttctcag ccatcgtaga gggcgacagc tttgaagaga
     3241 tctatcacaa agtgaaacgt gtcattgaag acctctcagg cccctacatc tgggtcccag
     3301 cccgagagag actcgaattc atggtgagca agggcgagga ggataacatg gccatcatca
     3361 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     3421 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     3481 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     3541 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     3601 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     3661 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     3721 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     3781 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     3841 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     3901 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     3961 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     4021 tgtacaagta aaagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     4081 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4141 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4201 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4261 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4321 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4381 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4441 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4501 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4561 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4621 ccgcctcccc gcatcgatac cgtcgacctc gacccgggcg gccgcttcga gcagacatga
     4681 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     4741 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     4801 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
     4861 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatctt cctagagcat
     4921 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg
     4981 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     5041 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat
     5101 taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5161 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5221 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     5281 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5341 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5401 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5461 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5521 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5581 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5641 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5701 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5761 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5821 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5881 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5941 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6001 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6061 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6121 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6181 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6241 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6301 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6361 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6421 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6481 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6541 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6601 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6661 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6721 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6781 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6841 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6901 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6961 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7021 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7081 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7141 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7201 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7261 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7321 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7381 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7441 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7501 ggttcctggc cttttgctgg ccttttgctc acatg
//
