LOCUS       sequence-241698-       11560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of TRIM33__RET fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-TRIM33__RET
FEATURES             Location/Qualifiers
     source          1..11560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5261..5272)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5506..5530)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6620..6640)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6694..6735
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6756..7323
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6773..7325
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6917..6934)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7144..7163
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7337..7936
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7337..7356)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7833..7853
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7950..8538
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8003..8023)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8421..8432)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8613..8846
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8965..8983)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9051..9155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9156..9224,9225..10016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9374..9393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10187..10775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10676..10695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10929..10946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11186..11207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11222..11227,11228..11245,11246..11252)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11260..11276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11265..11287
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11284..11300
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11284..11300
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11350..11369
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatggcg gaaaacaaag gcggcggcga ggctgagagc
     3481 ggcggcgggg gcagcggcag cgcgccggta actgccgggg ccgccgggcc cgccgcgcag
     3541 gaggcggagc cgcctctcac cgcggtgctg gtggaggagg aggaggagga aggcggcagg
     3601 gccggcgctg agggcggcgc ggccgggccc gacgacgggg gggtggccgc ggcctcctcg
     3661 ggctcggccc aggctgcttc atctcctgcg gcctcagtgg gcactggagt tgccgggggc
     3721 gcagtatcga cgccggctcc agctccagcc tcggctcccg ctccgggtcc ctcggcaggg
     3781 ccgcctcctg gaccgccagc ctcgctcctg gacacctgcg ccgtgtgtca gcagagcttg
     3841 cagagccggc gtgaggcgga gcccaagctg ctgccctgtc ttcactcctt ctgcctgcgc
     3901 tgcctgcccg agccggagcg ccagctcagc gtgcccatcc cggggggcag caacggcgac
     3961 atccagcaag ttggtgtaat acggtgccca gtatgccgcc aagaatgcag acagatagac
     4021 cttgtggata attattttgt gaaagacaca tctgaagctc ctagcagttc tgatgaaaaa
     4081 tcagaacagg tatgtactag ttgtgaagac aatgcaagtg cagttggctt ttgtgtagaa
     4141 tgtggagagt ggctatgtaa gacatgtatc gaagcacatc aaagagtaaa atttactaaa
     4201 gatcacttga tcaggaagaa agaagatgtc tcagagtctg ttggagcatc tggtcaacgc
     4261 cctgttttct gccctgtaca caaacaagaa cagttgaaac ttttctgtga aacatgtgat
     4321 agattgacat gtagagactg tcagctattg gaacacaaag aacataggta tcagtttttg
     4381 gaagaagctt ttcaaaatca gaagggtgca attgagaatc tactggcgaa acttcttgag
     4441 aagaagaatt atgttcattt tgcagctact caggtgcaga ataggataaa agaagtaaat
     4501 gagactaaca aacgagtaga acaggaaatt aaagtggcca ttttcaccct tatcaatgaa
     4561 attaataaga aaggaaaatc tctcttacaa cagctagaga atgttacaaa ggaaagacag
     4621 atgaagttac tacagcagca gaatgacatc acaggccttt cccggcaggt gaagcatgtt
     4681 atgaacttca caaattgggc aattgcaagt ggcagcagca cagcactact atacagcaag
     4741 cgactgatta ctttccagtt gcgtcatatt ttgaaagcac ggtgtgatcc tgtccctgct
     4801 gctaatggag caatacgttt ccattgtgat cccaccttct gggcaaagaa tgtagtcaat
     4861 ttaggtaatc tagtaataga gagtaaacca gctcctggtt atactcctaa tgttgtagtt
     4921 gggcaagttc ctccagggac aaaccacatt agtaaaaccc ctggacagat taacttagca
     4981 cagcttcgac tccagcacat gcaacaacaa gtatatgcac agaaacatca gcagttgcaa
     5041 cagatgagga tgcagcaacc accagcacct gtaccaacta caacaacaac aacacaacag
     5101 catcctagac aagcagcccc tcagatgtta caacaacagc ctcctcgatt gatcagtgtg
     5161 caaacaatgc aaagaggcaa catgaactgt ggagcttttc aagcccatca gatgagactg
     5221 gctcagaatg ctgccagaat accagggata cccaggcaca gcggccctca atattccatg
     5281 atgcagccac acctccaaag acaacactca aacccagggc atgctggacc ctttcccgta
     5341 gtatcggtac acaacaccac aatcaaccca acgagcccta ctacagcaac tatggcaaat
     5401 gcaaaccgag gtcccaccag cccatctgtt acagcaatag agctaatccc ctcagttacc
     5461 aatccagaaa accttccatc gctgccagat attccaccca tacagtccca gctttcttgt
     5521 acaaagtggt tcgaggatcc aaagtgggaa ttccctcgga agaacttggt tcttggaaaa
     5581 actctaggag aaggcgaatt tggaaaagtg gtcaaggcaa cggccttcca tctgaaaggc
     5641 agagcagggt acaccacggt ggccgtgaag atgctgaaag agaacgcctc cccgagtgag
     5701 cttcgagacc tgctgtcaga gttcaacgtc ctgaagcagg tcaaccaccc acatgtcatc
     5761 aaattgtatg gggcctgcag ccaggatggc ccgctcctcc tcatcgtgga gtacgccaaa
     5821 tacggctccc tgcggggctt cctccgcgag agccgcaaag tggggcctgg ctacctgggc
     5881 agtggaggca gccgcaactc cagctccctg gaccacccgg atgagcgggc cctcaccatg
     5941 ggcgacctca tctcatttgc ctggcagatc tcacagggga tgcagtatct ggccgagatg
     6001 aagctcgttc atcgggactt ggcagccaga aacatcctgg tagctgaggg gcggaagatg
     6061 aagatttcgg atttcggctt gtcccgagat gtttatgaag aggattcgta cgtgaagagg
     6121 agccagggtc ggattccagt taaatggatg gcaattgaat ccctttttga tcatatctac
     6181 accacgcaaa gtgatgtatg gtcttttggt gtcctgctgt gggagatcgt gaccctaggg
     6241 ggaaacccct atcctgggat tcctcctgag cggctcttca accttctgaa gaccggccac
     6301 cggatggaga ggccagacaa ctgcagcgag gagatgtact gcctgatgct gcaatgctgg
     6361 aagcaggagc cggacaaaag gccggtgttt gcggacatca gcaaagacct ggagaagatg
     6421 atggttaaga ggagagacta cttggacctt gcggcgtcca ctccatctga ctccctgatt
     6481 tatgacgacg gcctctcaga ggaggagaca ccgctggtgg actgtaataa tgcccccctc
     6541 cctcgagccc tcccttccac atggattgaa aacaaactct atggtagaat ttcccatgca
     6601 tttactagat tctagcaccc aacttttcta tacaaagtgg ttgatatcca gcacagtggc
     6661 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     6721 ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc taacgttact
     6781 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     6841 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6901 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6961 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     7021 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     7081 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     7141 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     7201 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     7261 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     7321 taatatggcc acacatatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     7381 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     7441 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     7501 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     7561 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     7621 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     7681 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     7741 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     7801 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     7861 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     7921 caagcccggt gcctaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa
     7981 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     8041 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     8101 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     8161 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8221 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     8281 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     8341 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     8401 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     8461 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     8521 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga
     8581 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8641 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     8701 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8761 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8821 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     8881 ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc
     8941 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     9001 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9061 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9121 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9181 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9241 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9301 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9361 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     9421 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9481 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9541 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9601 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9661 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9721 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9781 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9841 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9901 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9961 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10021 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10081 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10321 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10801 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10861 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10921 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10981 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
    11041 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    11101 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
    11161 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    11221 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    11281 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
    11341 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    11401 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    11461 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11521 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
