LOCUS       sequence-241703-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant transformation vector for stable expression of plastid 
            targeted GFP1-10 in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Sharma M, Kretschmer C, Lampe C, Stuttmann J, Klosgen RB
  TITLE     Targeting specificity of nuclear-encoded organelle proteins with a 
            self-assembling split-fluorescent protein toolkit.
  JOURNAL   J Cell Sci. 2019 Jun 3;132(11). pii: jcs.230839. doi: 
            10.1242/jcs.230839.
  PUBMED    31085714
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2019-06-3"; volume: "132(11). pii: jcs.230839. doi"; 
            pages: " 10.1242/jcs.230839"
COMMENT     Sequence Label: pLSU4cg10
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             169..810
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
ORIGIN
        1 atgaccaccg ctgtcaccgc cgctgtttct ttcccctcta ccaaaaccac ctctctctcc
       61 gcccgaagct cctccgtcat ttcccctgac aaaatcagct acaaaaaggt tcctttgtac
      121 tacaggaatg tatctgcaac tgggaaaatg ggacccatca gggccgcaat gtccaaagga
      181 gaagaactgt ttaccggtgt tgtgccaatt ttggttgaac tcgatggtga tgtcaacgga
      241 cataagttct cagtgagagg cgaaggagaa ggtgacgcca ccattggaaa attgactctt
      301 aaattcatct gtactactgg taaacttcct gtaccatggc cgactctcgt aacaacgctt
      361 acgtacggag ttcagtgctt ttcgagatac ccagaccata tgaaaagaca tgactttttt
      421 aagtcggcta tgcctgaagg ttacgtgcaa gaaagaacaa tttcgttcaa agatgatgga
      481 aaatataaaa ctagagcagt tgttaaattt gaaggagata ctttggttaa ccgcattgaa
      541 ctgaaaggaa cagattttaa agaagatggt aatattcttg gacacaaact cgaatacaat
      601 tttaatagtc ataacgtata catcactgct gataagcaaa agaacggaat taaagcgaat
      661 ttcacagtac gccataatgt agaagatggc agtgttcaac ttgccgacca ttaccaacaa
      721 aacaccccta ttggagacgg tccggtactt cttcctgata atcactacct ctcaacacaa
      781 acagtcctga gcaaagatcc aaatgaaaaa ggaacaggtg gcggcggaag ttag
//
