LOCUS       sequence-241716-       11251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PRKD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PRKD1
FEATURES             Location/Qualifiers
     source          1..11251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6188..6212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6265..6306
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6327..6894
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6344..6896
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6488..6505)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6715..6734
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6908..6910,6911..6913,6914..7627)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6908..6910,6911..6913,6914..7624)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6953..6974)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7214..7233)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7561..7582
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7641..8229
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7694..7714)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8112..8123)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8304..8537
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8656..8674)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8742..8846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8847..8915,8916..9707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9065..9084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9878..10466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10367..10386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10620..10637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10877..10898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10913..10918,10919..10936,10937..10943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10951..10967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10956..10978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10975..10991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10975..10991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11041..11060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg agcgcccctc cggtcctgcg gccgcccagt
     3481 ccgctgctgc ccgtggcggc ggcagctgcc gcagcggccg ccgcactggt cccagggtcc
     3541 gggcccgggc ccgcgccgtt cttggctcct gtcgcggccc cggtcggggg catctcgttc
     3601 catctgcaga tcggcctgag ccgtgagccg gtgctgctgc tgcaggactc gtccggggac
     3661 tacagcctgg cgcacgtccg cgagatggct tgctccattg tcgaccagaa gttccctgaa
     3721 tgtggtttct acggaatgta tgataagatc ctgctttttc gccatgaccc tacctctgaa
     3781 aacatccttc agctggtgaa agcggccagt gatatccagg aaggcgatct tattgaagtg
     3841 gtcttgtcag cttccgccac ctttgaagac tttcagattc gtccccacgc tctctttgtt
     3901 cattcataca gagctccagc tttctgtgat cactgtggag aaatgctgtg ggggctggta
     3961 cgtcaaggtc ttaaatgtga agggtgtggt ctgaattacc ataagagatg tgcatttaaa
     4021 atacccaaca attgcagcgg tgtgaggcgg agaaggctct caaacgtttc cctcactggg
     4081 gtcagcacca tccgcacatc atctgctgaa ctctctacaa gtgcccctga tgagcccctt
     4141 ctgcaaaaat caccatcaga gtcgtttatt ggtcgagaga agaggtcaaa ttctcaatca
     4201 tacattggac gaccaattca ccttgacaag attttgatgt ctaaagttaa agtgccgcac
     4261 acatttgtca tccactccta cacccggccc acagtgtgcc agtactgcaa gaagcttctg
     4321 aaggggcttt tcaggcaggg cttgcagtgc aaagattgca gattcaactg ccataaacgt
     4381 tgtgcaccga aagtaccaaa caactgcctt ggcgaagtga ccattaatgg agatttgctt
     4441 agccctgggg cagagtctga tgtggtcatg gaagaaggga gtgatgacaa tgatagtgaa
     4501 aggaacagtg ggctcatgga tgatatggaa gaagcaatgg tccaagatgc agagatggca
     4561 atggcagagt gccagaacga cagtggcgag atgcaagatc cagacccaga ccacgaggac
     4621 gccaacagaa ccatcagtcc atcaacaagc aacaatatcc cactcatgag ggtagtgcag
     4681 tctgtcaaac acacgaagag gaaaagcagc acagtcatga aagaaggatg gatggtccac
     4741 tacaccagca aggacacgct gcggaaacgg cactattgga gattggatag caaatgtatt
     4801 accctctttc agaatgacac aggaagcagg tactacaagg aaattccttt atctgaaatt
     4861 ttgtctctgg aaccagtaaa aacttcagct ttaattccta atggggccaa tcctcattgt
     4921 ttcgaaatca ctacggcaaa tgtagtgtat tatgtgggag aaaatgtggt caatccttcc
     4981 agcccatcac caaataacag tgttctcacc agtggcgttg gtgcagatgt ggccaggatg
     5041 tgggagatag ccatccagca tgcccttatg cccgtcattc ccaagggctc ctccgtgggt
     5101 acaggaacca acttgcacag agatatctct gtgagtattt cagtatcaaa ttgccagatt
     5161 caagaaaatg tggacatcag cacagtatat cagatttttc ctgatgaagt actgggttct
     5221 ggacagtttg gaattgttta tggaggaaaa catcgtaaaa caggaagaga tgtagctatt
     5281 aaaatcattg acaaattacg atttccaaca aaacaagaaa gccagcttcg taatgaggtt
     5341 gcaattctac agaaccttca tcaccctggt gttgtaaatt tggagtgtat gtttgagacg
     5401 cctgaaagag tgtttgttgt tatggaaaaa ctccatggag acatgctgga aatgatcttg
     5461 tcaagtgaaa agggcaggtt gccagagcac ataacgaagt ttttaattac tcagatactc
     5521 gtggctttgc ggcaccttca ttttaaaaat atcgttcact gtgacctcaa accagaaaat
     5581 gtgttgctag cctcagctga tccttttcct caggtgaaac tttgtgattt tggttttgcc
     5641 cggatcattg gagagaagtc tttccggagg tcagtggtgg gtacccccgc ttacctggct
     5701 cctgaggtcc taaggaacaa gggctacaat cgctctctag acatgtggtc tgttggggtc
     5761 atcatctatg taagcctaag cggcacattc ccatttaatg aagatgaaga catacacgac
     5821 caaattcaga atgcagcttt catgtatcca ccaaatccct ggaaggaaat atctcatgaa
     5881 gccattgatc ttatcaacaa tttgctgcaa gtaaaaatga gaaagcgcta cagtgtggat
     5941 aagaccttga gccacccttg gctacaggac tatcagacct ggttagattt gcgagagctg
     6001 gaatgcaaaa tcggggagcg ctacatcacc catgaaagtg atgacctgag gtgggagaag
     6061 tatgcaggcg agcaggggct gcagtacccc acacacctga tcaatccaag tgctagccac
     6121 agtgacactc ctgagactga agaaacagaa atgaaagccc tcggtgagcg tgtcagcatc
     6181 ctctagaacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     6241 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     6301 tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc
     6361 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     6421 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     6481 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     6541 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     6601 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     6661 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     6721 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     6781 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     6841 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     6901 cacacatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     6961 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     7021 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     7081 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     7141 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     7201 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     7261 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     7321 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     7381 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     7441 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     7501 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     7561 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     7621 caagtaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     7681 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7741 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7801 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7861 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7921 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7981 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     8041 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8101 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8161 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8221 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8281 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     8341 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     8401 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     8461 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     8521 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8581 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     8641 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     8701 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8761 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8821 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8881 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8941 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9001 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9061 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     9121 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9181 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9241 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9301 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9361 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9421 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9481 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9541 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9601 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9661 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9721 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9781 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9841 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9901 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9961 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10021 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10081 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10141 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10201 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10261 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10321 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10381 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10441 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10501 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10561 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10621 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10681 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
    10741 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10801 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
    10861 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10921 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10981 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
    11041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    11101 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    11161 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    11221 tttgttaaat atgtactaca aacttagtag t
//
