LOCUS       sequence-241733-       10855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Global labeling of excitatory synapses (green fluorescence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff 
            SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man HY, Han X
  TITLE     A Viral Toolbox of Genetically Encoded Fluorescent Synaptic Tags.
  JOURNAL   iScience. 2020 Jun 30;23(7):101330. doi: 10.1016/j.isci.2020.101330.
  PUBMED    32674057
REFERENCE   2  (bases 1 to 10855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-06-30"; volume: "23(7):101330. doi"; pages: " 
            10.1016/j.isci.2020.101330"
COMMENT     Sequence Label: Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC
FEATURES             Location/Qualifiers
     source          1..10855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2602..2613
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     promoter        2622..3911
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             4197..4220
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(4245..4247,4248..4955)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(4245..4247,4248..4955)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(4287..4308)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4548..4567)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4895..4916
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5001..5342
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             5391..5516
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     misc_feature    5547..6135
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5600..5620)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6018..6029)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6138..6154)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6139..6155)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6660..6840
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6866..6883)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6872..7096
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7142..7570
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7229..7248)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7439..7460
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7579..7599)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7584..7913
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7764..7899
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7826..7845
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7961..8008
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8027..8401
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8531..8652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8568..8587)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8622..8641
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8701..8717)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8701..8717)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8714..8736)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8725..8741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8749..8755,8756..8773,8774..8779))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8794..8815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8932..8949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9103..9691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9183..9202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9862..10653,10654..10722))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10485..10504
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10723..10827)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtcatcctc atcttaatta acattatggc cttaggtcac
     2641 ttcatctcca tggggttctt cttctgattt tctagaaaat gagatggggg tgcagagagc
     2701 ttcctcagtg acctgcccag ggtcacatca gaaatgtcag agctagaact tgaactcaga
     2761 ttactaatct taaattccat gccttggggg catgcaagta cgatatacag aaggagtgaa
     2821 ctcattaggg cagatgacca atgagtttag gaaagaagag tccagggcag ggtacatcta
     2881 caccacccgc ccagccctgg gtgagtccag ccacgttcac ctcattatag ttgcctctct
     2941 ccagtcctac cttgacggga agcacaagca gaaactggga caggagcccc aggagaccaa
     3001 atcttcatgg tccctctggg aggatgggtg gggagagctg tggcagaggc ctcaggaggg
     3061 gccctgctgc tcagtggtga cagatagggg tgagaaagca gacagagtca ttccgtcagc
     3121 attctgggtc tgtttggtac ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg
     3181 ctaaaaagca gggagagctg gaaagaaaca aggacagaga cagaggccaa gtcaaccaga
     3241 ccaattccca gaggaagcaa agaaaccatt acagagacta caagggggaa gggaaggaga
     3301 gatgaattag cttcccctgt aaaccttaga acccagctgt tgccagggca acggggcaat
     3361 acctgtctct tcagaggaga tgaagttgcc agggtaacta catcctgtct ttctcaagga
     3421 ccatcccaga atgtggcacc cactagccgt taccatagca actgcctctt tgccccactt
     3481 aatcccatcc cgtctgttaa aagggcccta tagttggagg tgggggaggt aggaagagcg
     3541 atgatcactt gtggactaag tttgttcaca tccccttctc caaccccctc agtacatcac
     3601 cctgggagaa caaggtccac ttgcttctgg gcccacacag tcctgcagta ttgtgtatat
     3661 aaggccaggg caacggagga gcaggttttg aagtgaaagg caggcaggtg ttggggaggc
     3721 agttaccggg gcaacgggaa cagggcgttt cggaggtggt tgccatgggg acctggatgc
     3781 tgacgaaggc tcgcgaggct gtgagcagcc acagtgccct gctcagaagc cccaagctcg
     3841 tcaatcaagc tggttctcca tttgcactca ggagcacggg caggcgagtg gcccctagtt
     3901 ctgggggcag cgggggatcc gccaccatgc tcgaagtcaa ggaagcatca ccaaccagca
     3961 tccagatcag ctgggtgctc cacttgcgcc acgttcgcta ctaccgcatc acctacggtg
     4021 aaactggtgg caatagccct gtccaggaat tcaccgtgcc tggcagcaag tccactgcta
     4081 ccatcagcgg cctgaaacct ggtgtcgact ataccatcac ggtgtacgcc gtcacgatct
     4141 tcagcgccta ccgctccgcc tggccgccga tctccatcaa ctaccgcacc ggaaccgatt
     4201 acaaggatga cgacgataag ggtagcggct ccagtagatc tggggtgagc aagggcgagg
     4261 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     4321 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     4381 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     4441 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     4501 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     4561 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4621 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4681 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     4741 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4801 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     4861 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     4921 ccgccgggat cactctcggc atggacgagc tgtactctag gggagctggc gctggagcgg
     4981 gtgcaggggc tggctctaga ttccagtgcc ggatctgcat gcggaacttc agcgaccggt
     5041 ccaacctgag caggcacatc agaacccaca ccggagaaaa gcccttcgcc tgcgacattt
     5101 gcggccggaa gttcgccatc agcagcaacc tgaacagcca caccaagatc cacactggca
     5161 gccagaaacc tttccagtgc agaatttgta tgagaaactt tagcagaagc gacaacctgg
     5221 ccagacacat ccggacacat actggtgaaa aaccttttgc ctgtgatatc tgtggcagaa
     5281 agtttgccac ctccggcaat ctgacccggc acacaaagat tcacctgcgg ggcagccagc
     5341 tatcgattgt cgacgctcct gaacaacgtg aaggtgcttc tcaagtttct gtttctgtta
     5401 cttttgaaga tgttgctgtt ctttttactc gtgatgaatg gaaaaaactt gatctttctc
     5461 aacgttctct ttatcgtgaa gttatgcttg aaaattattc taatcttgct tctatggctt
     5521 aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     5581 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5641 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5701 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5761 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5821 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5881 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5941 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6001 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6061 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6121 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     6181 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     6241 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     6301 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6361 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     6421 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     6481 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     6541 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     6601 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     6661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6841 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6901 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6961 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7021 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7081 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     7141 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7201 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7261 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7321 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7381 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7441 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7501 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     7561 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     7621 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     7681 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7741 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7801 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     7861 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     7921 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     7981 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     8041 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     8101 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     8161 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     8221 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     8281 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     8341 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     8401 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     8461 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     8521 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8581 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8641 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     8701 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8761 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9541 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10141 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10801 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
