LOCUS       sequence-241737-        3002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing inactive N- and C-terminal split fragments of 
            gp41-1 intein inserted into CcdA antitoxin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Beyer HM, Iwai H
  TITLE     Off-pathway-sensitive protein splicing screening based on a 
            toxin/antitoxin system.
  JOURNAL   Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139.
  PUBMED    30963690
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2019 Apr 8. doi: 10.1002/cbic.201900139."
COMMENT     Sequence Label: pHBSTC146
FEATURES             Location/Qualifiers
     source          1..3002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      128..716
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     617..636
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     870..887
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     903..922
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1908..1926)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1994..2098
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2099..2167,2168..2959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2317..2336)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
       61 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      121 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      181 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      241 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      301 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      361 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      421 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      481 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      541 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      601 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      661 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      721 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      781 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      841 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaacta
      901 ggcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
      961 atgcgtaagg agaaaatacc gcatcaggcc gcttaattaa cccgggccct ctagaattct
     1021 gcagcatgct tgccgcagcc gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga
     1081 gtatactcaa gcattgagtg gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc
     1141 ggttggtcac caaaagggag gggcaggcat gaagcagcgt attacagtga cagttgacag
     1201 cgacagctat cagttgctca aggcatacga tgtcaatatc tccggtctgg taagcacaac
     1261 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1321 gatggctgag gtcgcccggt ttattgaaat gaacggaggc ctggatctga aaacgcaggt
     1381 gcagacccca cagggcatga aagagatatc caacattcag gtgggcgatc ttgttctgag
     1441 caacaccggc tataacgagg tgctgaacgt gtttccgaaa agcaaaaaga aaagctataa
     1501 aattaccctg gaagatggca aagaaattat ttgtagcgaa gaacatctgt tcccgaccca
     1561 gaccggcgaa atgaacatca gcggcggtct gaaagagggc atgtgcctgt atgtgaaaga
     1621 ataagctttt aagaaggaga tatacatatg atgctgaaaa agattctgaa aattgaagag
     1681 ctggatgaac gcgaactgat cgatattgaa gtgagcggta accatctgtt ttatgcgaac
     1741 gacattctga cccacaatag cttcgccgac tgaatcgttt aaacgaattc actagtggat
     1801 ccgagctcgg taccaagctt gatgcatagc ttgagtattc taacgcgctg cattaatgaa
     1861 tcggccaacg cgcggggaga ggcggtttgc gtattgagca cgaaagggcc tcgtgatacg
     1921 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1981 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2041 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2101 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2161 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2221 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2281 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2341 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2401 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2461 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2521 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2581 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2641 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2701 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2761 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2821 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2881 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2941 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3001 aa
//
