LOCUS       sequence-241744-       11014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IDE__CAMK1D fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-IDE__CAMK1D
FEATURES             Location/Qualifiers
     source          1..11014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5184..5208)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6074..6094)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6148..6189
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6210..6777
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6227..6779
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6371..6388)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6598..6617
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6791..7390
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6791..6810)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7287..7307
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7404..7992
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7457..7477)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7875..7886)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8067..8300
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8419..8437)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8505..8609
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8610..8678,8679..9470)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8828..8847)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9641..10229
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10130..10149
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10383..10400
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10640..10661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10676..10681,10682..10699,10700..10706)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10714..10730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10719..10741
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10738..10754
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10738..10754
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10804..10823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgcgg taccggctag cgtggcttct gcaccccgca
     3481 ctgcccagca ccttccgctc agtcctcggc gcccgcctgc cgcctccgga gcgcctgtgt
     3541 ggtttccaaa aaaagactta cagcaaaatg aataatccag ccatcaagag aataggaaat
     3601 cacattacca agtctcctga agacaagcga gaatatcgag ggctagagct ggccaatggt
     3661 atcaaagtac ttcttatcag tgatcccacc acggataagt catcagcagc acttgatgtg
     3721 cacataggtt cattgtcgga tcctccaaat attgctggct taagtcattt ttgtgaacat
     3781 atgctttttt tgggaacaaa gaaataccct aaagaaaatg aatacagcca gtttctcagt
     3841 gagcatgcag gaagttcaaa tgcctttact agtggagagc ataccaatta ctattttgat
     3901 gtttctcatg aacacctaga aggtgcccta gacaggtttg cacagttttt tctgtgcccc
     3961 ttgttcgatg aaagttgcaa agacagagag gtgaatgcag ttgattcaga acatgagaag
     4021 aatgtgatga atgatgcctg gagactcttt caattggaaa aagctacagg gaatcctaaa
     4081 caccccttca gtaaatttgg gacaggtaac aaatatactc tggagactag accaaaccaa
     4141 gaaggcattg atgtaagaca agagctactg aaattccatt ctgcttacta ttcatccaac
     4201 ttaatggctg tttgtgtttt aggtcgagaa tctttagatg acttgactaa tctggtggta
     4261 aagttatttt ctgaagtaga gaacaaaaat gttccattgc cagaatttcc tgaacaccct
     4321 ttccaagaag aacatcttaa acaactttac aaaatagtac ccattaaaga tattaggaat
     4381 ctctatgtga catttcccat acctgacctt cagaaatact acaaatcaaa tcctggtcat
     4441 tatcttggtc atctcattgg gcatgaaggt cctggaagtc tgttatcaga acttaagtca
     4501 aagggctggg ttaatactct tgttggtggg cagaaggaag gagcccgagg ttttatgttt
     4561 tttatcatta atgtggactt gaccgaggaa ggattattac atgttgaaga tataattttg
     4621 cacatgtttc aatacattca gaagttacgt gcagaaggac ctcaagaatg ggttttccaa
     4681 gagtgcaagg acttgaatgc tgttgctttt aggtttaaag acaaagagag gccacggggc
     4741 tatacatcta agattgcagg aatattgcat tattatcccc tagaagaggt gctcacagcg
     4801 gaatatttac tggaagaatt tagacctgac ttaatagaga tggttctcga taaactcaga
     4861 ccagaaaatg tccgggttgc catagtttct aaatcttttg aaggaaaaac tgatcgcaca
     4921 gaagagtggt atggaaccca gtacaaacaa gaagctatac cggatgaagt catcaagaaa
     4981 tggcaaaatg ctgacctgaa tgggaaattt aaacttccta caaagaatga atttattcct
     5041 acgaattttg agattttacc gttagaaaaa gaggcgacac cataccctgc tcttattaag
     5101 gatacagcta tgagcaaact ttggttcaaa caagatgata agtttttttt gccgaaggct
     5161 tgtctcaact ttgaattttt cagtcccagc tttcttgtac aaagtggttc ggtgtccggt
     5221 ggagagctgt ttgaccggat agtggagaag gggttttata cagagaagga tgccagcact
     5281 ctgatccgcc aagtcttgga cgccgtgtac tatctccaca gaatgggcat cgtccacaga
     5341 gacctcaagc ccgaaaatct cttgtactac agtcaagatg aggagtccaa aataatgatc
     5401 agtgactttg gattgtcaaa aatggagggc aaaggagatg tgatgtccac tgcctgtgga
     5461 actccaggct atgtcgctcc tgaagtcctc gcccagaaac cttacagcaa agccgttgac
     5521 tgctggtcca tcggagtgat tgcctacatc ttgctctgcg gctaccctcc tttttatgat
     5581 gaaaatgact ccaagctctt tgagcagatc ctcaaggcgg aatatgagtt tgactctccc
     5641 tactgggatg acatctccga ctctgcaaaa gacttcattc ggaacctgat ggagaaggac
     5701 ccgaataaaa gatacacgtg tgagcaggca gctcggcacc catggatcgc tggtgacaca
     5761 gccctcaaca aaaacatcca cgagtccgtc agcgcccaga tccggaaaaa ctttgccaag
     5821 agcaaatgga gacaagcatt taatgccacg gccgtcgtga gacatatgag aaaactacac
     5881 ctcggcagca gcctggacag ttcaaatgca agtgtttcga gcagcctcag tttggccagc
     5941 caaaaagact gtctggcacc ttccacgctc tgtagtttca tttcttcttc gtcgggggtc
     6001 tcaggagttg gagccgagcg gagacccagg cccaccactg tgacggcagt gcactctgga
     6061 agcaagtgac acccaacttt tctatacaaa gtggttgata tccagcacag tggcggccgc
     6121 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     6181 gattctacgc gtaccggtta gtaatgagat ccctcccccc cccctaacgt tactggccga
     6241 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     6301 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     6361 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     6421 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     6481 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     6541 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     6601 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     6661 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     6721 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     6781 ggccacacat atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6841 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6901 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6961 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7021 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7081 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7141 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7201 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7261 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7321 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     7381 cggtgcctaa atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga
     7441 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7501 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7561 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7621 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7681 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7741 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7801 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7861 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7921 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7981 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag
     8041 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     8101 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8161 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8221 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8281 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8341 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc
     8401 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     8461 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     8521 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     8581 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8641 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8701 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8761 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8821 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     8881 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8941 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     9001 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     9061 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     9121 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     9181 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     9241 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     9301 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     9361 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     9421 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     9481 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     9541 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9601 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9661 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9721 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9781 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9841 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9901 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9961 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    10021 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    10081 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    10141 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    10201 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    10261 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    10321 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    10381 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    10441 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc
    10501 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    10561 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
    10621 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    10681 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    10741 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta
    10801 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
    10861 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
    10921 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
    10981 gtatttgtta aatatgtact acaaacttag tagt
//
